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Rashidi Monfared, S., Mirvaghefi, A., Farahmand, H., Nematollahi, M., Poorbakhsh, S., Ashtari, A. (2013). Detection of Vertical Transmission of the Bacterium Streptococcus Agalactiae in Rainbow Trout (Oncorhynchus Mykiss) Brooders Using the PCR of the Gene Scpb. , 66(1), 49-58. doi: 10.22059/jfisheries.2013.35463
Samira Rashidi Monfared; Alireza Mirvaghefi; Hamid Farahmand; Mohamadali Nematollahi; Seyed Ali Poorbakhsh; Abbas Ashtari. "Detection of Vertical Transmission of the Bacterium Streptococcus Agalactiae in Rainbow Trout (Oncorhynchus Mykiss) Brooders Using the PCR of the Gene Scpb". , 66, 1, 2013, 49-58. doi: 10.22059/jfisheries.2013.35463
Rashidi Monfared, S., Mirvaghefi, A., Farahmand, H., Nematollahi, M., Poorbakhsh, S., Ashtari, A. (2013). 'Detection of Vertical Transmission of the Bacterium Streptococcus Agalactiae in Rainbow Trout (Oncorhynchus Mykiss) Brooders Using the PCR of the Gene Scpb', , 66(1), pp. 49-58. doi: 10.22059/jfisheries.2013.35463
Rashidi Monfared, S., Mirvaghefi, A., Farahmand, H., Nematollahi, M., Poorbakhsh, S., Ashtari, A. Detection of Vertical Transmission of the Bacterium Streptococcus Agalactiae in Rainbow Trout (Oncorhynchus Mykiss) Brooders Using the PCR of the Gene Scpb. , 2013; 66(1): 49-58. doi: 10.22059/jfisheries.2013.35463

Detection of Vertical Transmission of the Bacterium Streptococcus Agalactiae in Rainbow Trout (Oncorhynchus Mykiss) Brooders Using the PCR of the Gene Scpb

شیلات
Article 5, Volume 66, Issue 1, April 2013, Pages 49-58    PDF (615.68 K)
Document Type: Research Paper
DOI: 10.22059/jfisheries.2013.35463
Authors
Samira Rashidi Monfared1; Alireza Mirvaghefi* 1; Hamid Farahmand1; Mohamadali Nematollahi1; Seyed Ali Poorbakhsh2; Abbas Ashtari2
1Department of Fisheries, Faculty of Natural Resources, University of Tehran, Karaj, Iran
2Razi Vaccine and Serum Research Institute
Abstract
Streptococcus agalactiae (group B, GBS) is an important human and animal pathogen that
causes invasive infection in fish. The present study aims to investigate the detection of vertical
transmission S. agalactiae using the PCR of the gene scpB in the ovarian tissues. A group of 48
fishes were injected intramuscularly (i.m) with 0.5 ml bacterial suspension containing 3.5×107
CFU/0.5 ml in normal saline, This group was compared with a control group of 48 fishesthat
were injected with 0.5 ml sterile saline. In this study kidney tissue, ovarian tissue and ovarian
fluid were sampled from the fourth day after injection. DNA was extracted from both the direct
tissue and the bacteria isolated from the tissues, which were cultured in the BHI medium. The
extracted DNA was then used in the PCR. The PCR confirmed the existence of this bacterium
in 91.46% (44/48) and 68.75% (34/48) of the kidney and the ovarian tissues, respectively,
and 95.83% (46/48) and 87.5% (42/48) of bacteria isolated from the kidney and the ovarian
tissue cultures, respectively. The presence of S. agalactiaein rainbow trout ovarian tissue was
confirmed by PCR and sequencing that can be the evidence for vertical transmission of this
bacterium in rainbow trout brood stock.
Keywords
Oncorhynchus mykiss; PCR; scpB gene; Streptococcus agalactiae; vertical transmission
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