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Pahlevan Kakhki, M.. (1392). TRIzol-based RNA Extraction: A Reliable Method for Gene Expression Studies. , 25(1), 13-17.
M. Pahlevan Kakhki. "TRIzol-based RNA Extraction: A Reliable Method for Gene Expression Studies". , 25, 1, 1392, 13-17.
Pahlevan Kakhki, M.. (1392). 'TRIzol-based RNA Extraction: A Reliable Method for Gene Expression Studies', , 25(1), pp. 13-17.
Pahlevan Kakhki, M.. TRIzol-based RNA Extraction: A Reliable Method for Gene Expression Studies. , 1392; 25(1): 13-17.

TRIzol-based RNA Extraction: A Reliable Method for Gene Expression Studies

Journal of Sciences, Islamic Republic of Iran
مقاله 2، دوره 25، شماره 1، خرداد 2014، صفحه 13-17    اصل مقاله (444.44 K)
نوع مقاله: Original Paper
نویسنده
M. Pahlevan Kakhki
Department of Basic Sciences, Faculty of Medicine, Gonabad University of Medical Sciences, Gonabad, Islamic Republic of Iran
چکیده
RNA extraction is a prerequisite technique for gene expression studies, analyzing the etiology and disease progression, treatment effects, as well as designing the diagnostic methods. Although many RNA extraction kits have been commercialized, but these kits are expensive and are not accessible in some countries. Many studies have shown that TRIzol is an applicable material for the RNA extraction from various biological samples. In this study, evaluation of the initial TRIzol volume and -20˚C temperature on the purity and solubility of RNA, which followed by measurement of IL-1B expression shows that TRIzol based RNA extraction is a “reliable” method for gene expression studies.
کلیدواژه‌ها
RNA؛ TRIzol volume؛ Purity؛ Gene expression, IL-1B
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