Gene ontology of Trichoderma harzianum differential ESTs during colonization of tomato spermosphere and rhizoplane | ||
| دانش گیاهپزشکی ایران | ||
| Article 16, Volume 45, Issue 1, May 2014, Pages 171-184 PDF (1.54 M) | ||
| Document Type: Research Paper | ||
| DOI: 10.22059/ijpps.2014.52258 | ||
| Authors | ||
| Mehdi Mehrabi-Koushki* ; Hamid Rouhani1; Esmat Mahdikhani moghaddam2 | ||
| 1Professor of Ferdowsi University of Mashhad | ||
| 2Ferdowsi University of mashhad | ||
| Abstract | ||
| Some Trichoderma strains are used as biocontrol agents against phytopathogens. Differential display reverse transcriptase PCR has been developed to detect differentially expressed genes of T. harzianum T7 during colonization stages of tomato spermosphere and rhizoplane. DDRT-PCR products were diplayed on gel and 62 differential bands excised, purified, cloned, and sequenced. Obtained ESTs were submit-queried to NCBI database by BLAST2GO search and evaluated their gene ontology. Most of the transcripts corresponded to known or hypothetical proteins. These proteins correspond to different functional groups, which play role in metabolism, signaling both within and between cells, host interaction, protein transport, translation, controlling of cell growth and survival, adaptation to environmental variables, transportation of biomolecules between nucleus and cytoplasm, regulating protein function, cell division, catalyzing analogous reactions, repairing damaged DNA molecules, and other vital functions in different organisms. Some of the detected ESTs in this study corresponded to genes encodes enzymes potentially involved in nutritional support of Trichoderma in its ecological niches. | ||
| Keywords | ||
| BLAST2GO; ESTs; DDRT-PCR and Gene Function | ||
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