طباطبایی, سید علی. (1393). اثر پیشتیمار بذر جو با سالیسیلیک اسید بر رشد گیاهچه، مقدار پرولین و فعالیت آنزیمهای آنتیاکسیدانت تحت شرایط تنش خشکی. , 16(2), 475-486. doi: 10.22059/jci.2014.53056
سید علی طباطبایی. "اثر پیشتیمار بذر جو با سالیسیلیک اسید بر رشد گیاهچه، مقدار پرولین و فعالیت آنزیمهای آنتیاکسیدانت تحت شرایط تنش خشکی". , 16, 2, 1393, 475-486. doi: 10.22059/jci.2014.53056
طباطبایی, سید علی. (1393). 'اثر پیشتیمار بذر جو با سالیسیلیک اسید بر رشد گیاهچه، مقدار پرولین و فعالیت آنزیمهای آنتیاکسیدانت تحت شرایط تنش خشکی', , 16(2), pp. 475-486. doi: 10.22059/jci.2014.53056
طباطبایی, سید علی. اثر پیشتیمار بذر جو با سالیسیلیک اسید بر رشد گیاهچه، مقدار پرولین و فعالیت آنزیمهای آنتیاکسیدانت تحت شرایط تنش خشکی. , 1393; 16(2): 475-486. doi: 10.22059/jci.2014.53056
اثر پیشتیمار بذر جو با سالیسیلیک اسید بر رشد گیاهچه، مقدار پرولین و فعالیت آنزیمهای آنتیاکسیدانت تحت شرایط تنش خشکی
استادیار، بخش زراعت، مرکز تحقیقات کشاورزی و منابع طبیعی استان یزد، یزد – ایران
چکیده
یکی از تأثیرات کاهش آب در خاک، کاهش رشد و نمو گیاهچههای سبزشده و تغییر در نمو مزرعهای آنها در چنین شرایطی است. مشخص شده که پیشتیمار بذرها موجب بهبود برخی خصوصیات گیاهچه و گیاه حاصل میشود. بهمنظور بررسی اثر پیشتیمار بذر جو با سالیسیلیک اسید بر رشد گیاهچۀ جو، مقدار پرولین و تغییر فعالیت آنزیمهای آنتیاکسیدانتی تحت شرایط تنش خشکی آزمایشی در سال 1391 در مرکز تحقیقات کشاورزی و منابع طبیعی استان یزد به اجرا درآمد. فاکتور اول شامل غلظتهای مختلف سالیسیلیک اسید (صفر، 50، 100 و 150 قسمت در میلیون) و فاکتور دوم شامل سه سطح خشکی (صفر، 6- و 12- بار) بود. نتایج نشان داد که اثر سالیسیلیک اسید و تنش خشکی بر درصد جوانهزنی، سرعت جوانهزنی، وزن خشک گیاهچه، پروتئین، کاتالاز، آسکوربات پراکسیداز و پرولین معنادار بود. اثر متقابل تنش و تیمار بذر بر تعداد برگ معنادار نبود، ولی تأثیرات اصلی آنها معنادار بود. بیشترین درصد جوانهزنی (7/98 درصد)، سرعت جوانهزنی و وزن خشک گیاهچه مربوط به غلظت 100 قسمت در میلیون سالیسیلیک اسید بود. تحت شرایط تنش خشکی، پروتئین کاهش یافت که استفاده از سالیسیلیک اسید مقدار آن را افزایش داد. همچنین تحت شرایط تنش فعالیت کاتالاز، آسکوربات پراکسیداز و پرولین افزایش یافت که استفاده از سالیسیلیک اسید سبب کاهش در آنها شد.
Effect of salicylic acid pretreatment of barley seed on seedling growth, proline content and antioxidant enzymes activity under drought stress
نویسندگان [English]
sayed ali tabatabaei
Assistant Profossor, Department of Agronomy, Agriculturale and Natural Resources Research Center of Yazd, Yazd, Iran
چکیده [English]
One of the effects of reducing water content on soil is reduction of growth and development of seedlings and variation of field development of them. It was found that seed priming improves some characteristics of seedling and plant. An experiment was conducted for evaluating the effect of seed priming by salicylic acid on some characteristics of barley seed at early growth stages, antioxidant enzyes activity and proline content under drought stress. The first factor was priming with salicylic acid (zero, 50, 100 and 150 ppm) and the second factor was three levels of drought (zero, -6 and -12 bar). The results showed that SA pretreatment effect on germination percentage, germination rate, dry weight, protein, catalase, ascorbat peroxidase activity and proline content were significant. The interaction effect of stress seed treatment on leaf number was not significant but the main factors were significant. The highest germination percentage (98.7), germination rate and seedling dry weight were found at 100 ppm concentrations of salicylic acid. Under drought stress conditions, protein content decreased but salicylic acid pretreatment increased the valuet. Also, drought stress conditions increased enzyme activity and proline content, but salicylic acid pretreatment decreased them.
انصاری ا و شریفزاده ف (1391) پرایمینگ بذور چاودار با استفاده از تیمارهای هورمونی و اسمزی و تأثیر تیمارهای پس از پرایمینگ بر کیفیت و طول عمر بذور پرایمشده. پردیس کشاورزی و منابع طبیعی دانشگاه تهران. کرج. پایاننامۀ کارشناسی ارشد.
خدابنده ن (1389) غلات. مؤسسۀ انتشارات و چاپ دانشگاه تهران. چاپ 10. 574 ص.
Aberg B (1981) Plant growth regulators XLI. Monosubstituted benzoic acid. Swedian Journal of Agriculture Research. 11: 93-105.
Allen RD (1995) Dissection of oxidative stress tolerance using transgenic plants. Plant Physiology.57: 1049-1054.
Ashraf M and Rauf H (2001) Inducing salt tolerance in maize (Zea mays L.) through seed priming with chloride salts: growth and ion transport at early growth stages. Acta Physiologiae Plantarum. 23: 407-414.
Ansari O, Azadi MS, Sharif-Zadeh F and Younesi E (2013) Effect of hormone priming on germination characteristics and enzyme activity of mountain rye (Secale montanum) seeds under drought stress conditions. Stress Physiology and Biochemistry. 9(3): 61-71.
Ansari O, Choghazardi HR, Sharif Zadeh F and Nazarli H (2012) Seed reserve utilization and seedling growth of treated seeds of mountain rye (Secale montanum) as affected by drought stress. Cercetări Agronomice în Moldova. 2 (150): 43-48.
AnsariOand Sharif-Zadeh F (2012) Does Gibberelic acid (GA), Salicylic acid (SA) and Ascorbic acid (ASc) improve Mountain Rye (Secalemontanum) Seeds Germination and Seedlings Growth under Cold Stress?. International Research Journal of Applied and Basic Sciences. 3(8): 1651-1657.
Basra SMA, Farooq M, Wahid A and Khan M B (2006) Rice seed invigoration by hormonal and vitamin priming. Seed Science and Technology. 34: 755-780.
Bates LS Waldern RP and Teave ID (1973) Rapid determination of free proline for water stress studies. Plant and Soil. 39: 205-207.
Bian, YM, Chen SY, Liu SK and Xie MY (1988) Effects of HF on praline of some plants. Plant Physiology Communications. 6: 19-21.
Bradford M (1976) A rapid and sensitive method for the quantitation of protein utilizing the principle of protein-dye binding. Annual Review of Biochemistry. 72: 248-254.
Canakcl S and Munzuroglu O (2002) Effect of acetyl salicylic acid application to the roots of bean (Phaseolus vulgariz L.) and corn (Zeamays L.) seedlings on transpiration rate and weight changes. Journal of Science and Engineering. 14(2): 1-9.
Falleri E (1994) Effect of water stress on germination in six provenances of Pinus pinaster Ait. Seed. Science and Technology. 22: 591-599.
Gambel PE and Burke JJ (1984) Effect of water stress on the chloroplast antioxidant system. I. Alteration in glutathione reductase activity. Plant Physiology. 76: 615- 621.
Georgieva MD, Djilianov D, Konstantinova T and Parvanova D (2004) Screening of Bulgarian raspberry cultivars and elites for osmotic tolerance in vitro. Biotechnology Equip. 18(2): 95-98.
Hong Z, Lakkineni K, Zhang Z and Verma DS (2000) Removal of feedback inhibition of 1- pyrrolin -5-carboxylate synthetas resalts in increased prolin accumulation and prodaction of plant from osmotic stress. Plant Physiology. 122: 1129-1136.
Hus JL and Sung JM (1997) Antioxidant role of glutathione associated with accelerated aging and hydration of triploid Waremelon seeds. Physiological Plantrum. 100: 967-974.
Hegedus A, Erdei S and Horvath G (2001) Comparative studies of H2O2 detoxifying enzymes in green and greening barley seedlings under cadmium stress. Plant Science. 160: 1085-1093.
Iqbal M and Ashraf M (2007) Seed treatment with auxins modulates growth and ion partitioning in salt-stressed wheat plants. Integrative Plant Biology. 49: 1003-1015.
Janda T, Szalai G, Tari I and Paldi E (1999) Hydroponic treatment with salicylic acid decreases the effects of chilling injury in maize (Zea mays L.) plants. Planta. 208: 175-180.
Johnson LB and Cunningham BA (1972) Peroxidase activity in healthy and leaf-rustinfected wheat leaves. Phytochemistry. 11: 547-551.
Kao CH (1981) Senescense of rice leaves. VI. Comparative study of the metabolic changes of senescing turgid and water–stressed excised leaves. Plant and Cell Physiology. 22: 683-685.
Kukreja S Nandval AS Kumar N, Sharma SK, Sharma SK, Unvi V and Sharma PK (2005) Plant water status, H2O2 scavenging enzymes, ethylene evolution and membrane integrity of Cicer arietinum roots as affected by salinity. Biology Plantarum. 49: 305-308.
Lascano HR, Antonicelli GE, Luna CM, Melchiorre MN, Gomez LD, Racca RW, Trippi VS and Casano LM (2005) Antioxidant system response of different wheat cultivars under drought : field and in vitro studies. Australian Journal of Plant Physiology. 28: 1095-1102.
Leport L, Turner NC, French RJ, Barr MD, Duda R, Davies SL, Tennant D and Siddique KHM (1999) Physiological responses of chickpea genotypes to terminal drought in a Mediterranean-type environment. European Journal of Agronomy. 11: 279-291.
Martin M, Micell F, Morgan JA, Scalet M and Zerbi G (1993) Synthesis of osmotically active substances in winter wheat leaves as related to drought resistance of different genotypes. Agronomy and Crop Science. 171: 176-184.
Patade VY, Maya K and Zakwan A (2011) Seed priming mediated germination improvement and tolerance to subsequent exposure to cold and salt stress in capsicum. Research Journal of Seed Science. 4 (3): 125 -136.
Pignocchi C and Foyer CH (2003) Apoplastic ascorbate metabolism and its role in the regulation of cell signaling. Current Opinion in Plant Biology. 6: 379-389.
Sairam RK and Saxena DC (2000) Oxidative stress and antioxidant in wheat genotypes: possible mechanism of water stress tolerance. Journal of Agronomy and Crop Science. 184: 55-61.
Sairam RK and Srivastava GC (2002) Changes in antioxidant activity in sub-cellular fractions of tolerant and susceptible wheat genotypes in response to long term salt stress. Plant Science. 162: 897-904.
Sairam RK and Srivastava GC (2001) Water stress tolerance of wheat Triticum aestivum L.: Variation in hydrogen peroxide accumulation and antioxidant activiy in tolerant and susceptible genotype. Journal of Agronomy and Crop Science. 186: 63-70.
Schneider EC and Gupta SC (1985) Corn emergence as influenced by soil temperature, matric potential and aggregate size distribution. Soil Science Society of America. 49: 415-422.
Sgherri CLM, Maffei M and Navari-Izzo F (2000) Antioxidative enzymes in wheat subjected to increasing water deficit and rewatering. Plant Physiology. 157:273-279.
Trautwein EA, Reickhoff D and Erbershobler HF (1997) The cholesterol- lowering effect of Psyllium a source dietary fiber. Ernhurung Umschau. 44: 214-216.