پیوندهای مفید
اخبار و اعلانات
آمار
| تعداد نشریات | 161 |
| تعداد شماره‌ها | 6,532 |
| تعداد مقالات | 70,502 |
| تعداد مشاهده مقاله | 124,118,440 |
| تعداد دریافت فایل اصل مقاله | 97,224,486 |
بررسی ویژگی‌های زیستی باکتریPseudomonas fluorescens UTPF68 و ارزیابی توان بیوکنترلی آن علیه Phytophthora drechsleri عامل بوته‌میری خیار | ||
| کنترل بیولوژیک آفات و بیماری های گیاهی | ||
| مقاله 3، دوره 3، شماره 2، مهر 1393، صفحه 105-116 اصل مقاله (497.41 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22059/jbioc.2014.53466 | ||
| نویسندگان | ||
| سمیه السادات قافله باشی1؛ فاطمه جمالی2؛ مسعود احمدزاده* 3 | ||
| 1دانش‌آموختة کارشناسی ارشد، گروه گیاه‌پزشکی، دانشکدة علوم و مهندسی کشاورزی، پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج | ||
| 2استادیار، گروه زراعت و اصلاح نباتات، دانشکدة کشاورزی و منابع طبیعی، دانشگاه خلیج فارس بوشهر | ||
| 3استاد، گروه گیاه‌پزشکی، دانشکدة علوم و مهندسی کشاورزی، پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج | ||
| چکیده | ||
| در این تحقیق از باکتری Pseudomonas fluorescens UTPF68، عامل کنترل بیولوژیک، علیه Phytophthora drechsleri استفاده شد. ترکیبات فرّار سویة UTPF68 در سه محیط کشت NA، NAG و PDA به‌ترتیب 44/84 درصد، 55/83 درصد و 33/67 درصد بازدارندگی از رشد میسیلیومی P. drechsleri را در برداشت. نتایج نشان داد که متابولیت‌های خارج‌سلولی تولیدشده توسط سویة UTPF68 در محیط کشت PDA به‌طور کامل از رشد P. drechsleri ممانعت و در محیط کشت CMA به میزان 3/93 درصد از رشد بیمارگر جلوگیری کرد. سلول و عصارة سویة UTPF68 در سه غلظت 10 درصد، 25 درصد و 50 درصد، درون محیط مایع، به‌طور کامل از رشد P. drechsleriجلوگیری کرد. همچنین، این باکتری توانست هر سه آنتی‌بیوتیک DAPG، MAPG و Plt را در محیط کشت KBG تولید کند. نتایج نشان داد که این باکتری می‌تواند هر دو شرایط in vitro و in vivo را به کنترل P. drechsleri درآورد؛ به‌طوری که، کاربرد غلظت 108 سلول در هر میلی‌لیتر از این باکتری در خاک، خسارت ناشی از این بیمارگر را تا 60 درصد کاهش داد. مایه‌زنی این باکتری با خاک در حضور و نبود بیمارگر باعث بهبود فاکتورهای رشدی گیاه تحت شرایط گلخانه شد. به‌طوری که، از این لحاظ با شاهد سالم تفاوت معنی‌داری نشان داد. بررسی کلونیزاسیون ریشة خیار نشان داد که این باکتری بعد از گذشت دو هفته، توانست منطقة ریزوسفر با جمعیت 109×3/2 در گرم ریشه را کلونیزه کند. | ||
| کلیدواژه‌ها | ||
| آنتی‌بیوتیک؛ عصارة سلولی؛ کلونیزاسیون؛ کنترل بیولوژیک؛ متابولیت‌های خارج‌سلولی | ||
| عنوان مقاله [English] | ||
| Study biological properties of Pseudomonas fluorescens UTPF68, biocontrol agent against Phytophthora drechsleri | ||
| نویسندگان [English] | ||
| Somayeh Sadat Ghafelebashi1؛ Fatemeh Jamali2؛ Masoud Ahmadzadeh3 | ||
| 2Department of Agronomy and Plant Breeding, Faculty of Agriculture and Natural Resources, Perisan Gulf University, Bushehr | ||
| 3Professor, Department of Plant Protection, Faculty of Science and Agricultural engineering, University College of Agriculture and Natural resources, University of Tehran, Karaj | ||
| چکیده [English] | ||
| Pseudomonas fluorescens UTPF68, well-known biocontrol bacteria was used in this study. The volatile metabolites released from UTPF68 in NA, NAG, PDA media, could inhibit the growth of fungi P.drechsleri at the rate of 84.44, 83.55 and 67.33 percent, respectively. The results showed that antibiotics production in PDA and CMA media prevented the growth of P. drechsleri, 100% and 93%, respectively. The results showed that this strain is able to produce all three antibiotics DAPG, MAPG and the Plt. Also, the extracellular extracts of UTPF68 reduced the vegetative growth of P.drechsleri in extracts concentration. In addition, the bacterium effectiveness against the pathogen in greenhouse cucumber was studied. The bacterium under the in vitro and in vivo conditions was able to control the pathogen; So that, the application of bacterial concentration at 108 CFU/ml in to the soil, reduced the damage resulted from disease up to 60%. Also, the use of this bacterium, in the presence and absence of the pathogen as controls, increased the growth of cucumber. The bacterial colonization experiment showed that the population density of strain UTPF68 was increased on cucumber root system to 2.3×109 CFU. | ||
| کلیدواژه‌ها [English] | ||
| Antibiotic, Biocontrol, Colonization, extracellular extracts, the volatile metabolites | ||
| مراجع | ||
|
Ahmadzadeh M , Ghasemi S (2012) Introduction of Pseudomonas fluorescens as a new biocontrol agent in Iran. Biological Control of Pests and Plant Diseases 1: 49-60. (In Persian).
Alavi A (1973) Root rot disease of cucumbers. Iranian Journal of Plant Pathology 9: 37-49 (In Persian).
Azad Disfani F (2001) Effect of some antagonistic bacteria against Verticillium dahlia, Cotton wilt disease. M.Sc., Tehran University, University College of Agricultural and Natural Resources, Iran. (In Persian).
Bangera M G & Thomashow LS (1999) Identification and characterization of a gene cluster for synthesis of the polyketide antibiotic 2, 4-diacetylphloroglucinol from Pseudomonas fluorescens Q2-87. Journal of Bacteriology 181(10): 3155-3163.
Baligh M, Delgado MA, Conway KE (1999) Evaluation of Burkholderia cepacia strains: Root colonization of Catharanthus roseus and in vitro inhibitation of selected soil-born fungal pathogens. Proceedings of the Oklahoma Academy of Science 79: 19-27.
Castric KF , Castric P (1983) Method for rapid detection of cyanogenic bacteria. Applied and Environmental Microbiology 45: 701-702.
Chin-A-Woeng TFC, Priester AJV , Lugtenberg BJJ (1997) Description of colonization of a gnotobiotic tomato rhizosphere by Pseudomonas fluorescens biocontrol strain WCS365, using scanning electron microscopy. Molecular Plant-Microbe Interactions Journal 10:79-86.
De Lafuente L, Quagliotto L, Bajsa N, Fabiano E, Altier N, Arias A (2001) Inoculation with Pseudomonas fluorescens biocontrol strains does not affect the symbiosis between rhizobia and forage legumes. Soil Biology and Biochemistry 34: 545–548.
Duffy BK & Défago G (1999) Environmental factors modulating antibiotic and siderophore biosynthesis by Pseudomonas fluorescens biocontrol strains. Applied & Environmental Microbiology 65(6): 2429-2438.
Ershad J, Mostofipour P (1969) Root rot of cucurbits in Iran. Iranian Journal of Plant Pathology 5: 38-45 (In Persian).
Ghafelebashi S (2012) Optimization of culture conditions of Pseudomonas fluorescens UTPF68 using the response surface methodology. M.Sc., University of Tehran, University College of Agricultural and Natural Resources, Iran. (In Persian).
Hagedron C, Gould WD, Bradinelli RT (1989) Rhizobacteria of cotton and their repression of seedling disease pathogens. Applied and Environmental Microbiology 55: 279-2797.
Howell CR & Stipanovic RD (1980) Suppression of Pythium ultimum induced damping-off of cotton seedlings by Pseudomonas fluorescens and its antibiotic, pyoluteorin. Phytopathology 70(8): 712-715.
Hwang J, Beneson DM (2005) Identification, sensitivity and compatibility types of Phytophthora spp. attacking floricultur crops in North Carolina. Plant Disease 89: 185-190.
Jamali F (2009) Influence of some biotic factors on the expression of hydrogen cyanide- and 2, 4- diacetylphloroglucinol biosynthesis genes in Pseudomonas fluorescens on bean rhizosphere. Ph. D., dissertation, University of Tehran, University College of Agricultural and Natural Resources, Iran, (In Persian).
Keel C, Defago G (1997) Interaction between beneficial soil bacteria and root pathogens: mechanism and ecological impact In: Gange, A.C. and Brown, V. K. (eds.). multitrophic interaction in terrestrial system pp. 27-46. Blackwell Scientific publishers, London, U.K.
Kim E, Hwang BK (1992) Virulence to Korean pepper cultivars of isolates of Phytophthora capsici from different geographic areas. Plant Disease76: 486-489.
Kraus L & Loper JE (1992) Lack of evidence for a role of antifungal metabolite production by Pseudomonas fluorescens Pf-5 in biological control of Pythium damping-off of cucumber. Phytopathology 82(3): 264-271.
Lemanceau P, Corberand T, Gardan L, Latour L, Laguerre G, Boeufgras J M, Albouvette C (1995) Effect of two plant species flax (Linum usitatissinum L.) and tomato (Lycopersicon esculentum Mill.) on the diversity of soilborne populations of fluorescent pseudomonads. Applied & Environmental Microbiology 61(3): 1004-1012.
Liu L, Kloepper JW, Tuzun S (1995) Introduction of systemic resistance in cucumber against Fusarium wilt by plant growth-promoting rhizobacteria. Phytopathology. 85: 695-698.
Maurhofer M, Keel C, Schnider U, Voisard C, Défago G (1992) Influence of enhanced antibiotic production in Pseudomonas fluorescens strain CHA0 on its disease suppressive capacity. Phytopathology 82(2): 190-195.
Maurhofer M, Keel C, Haas D, Défago G (1994) Pyoluteorin production by Pseudomonas fluorescens strain CHA0 is involved in the suppression of Pythium damping-off of cress, but not cucumber. European Journal of Plant Pathology 100(3, 4): 221-232.
Notz R, Maurhofer M, Schnider-Keel U, Duffy B, Haas D, Défago G (2001) Biotic factors affecting expression of the 2,4-diacetylphloroglucinol biosynthesis gene phlA in Pseudomonas fluorescens biocontrol strain CHA0 in the rhizosphere. Phytopathology 91(9): 873-881.
Rabindran R, Vidhysaekaran P (1996) Development for formulation of Pseudomonasfluorescens PfALR2 for management of rice sheath blight. Crop Protection 15(8): 715- 721.
Savchuk S, Fernando WGD, Park PS (2001) Potential for biocontrol of Sclerotiniasclerotiorum on canola. Canadian Journal of Plant Pathology 23:205.
Sperber JI (1958) the incidence of apatite-solubilizing oranisms in the rhizorsrhere and soil. Australian Journal of Agriculture Reearch 9: 778.
Tucker C M (1931) Taxonomy of the genus Phytophthora de Bary. University of Missouri Agricultural Experimental Station Research Bulletin 153- 158 pp.
Vessey KJ (2003) Plant growth- promoting rhizobacteria as biofertilizers. Plant Soil 255: 571-580.
Wuthrich B, Defago G (1990) Suppression of wheat take-all and black root rot of tobacco by Pseudomonas fluorescens strain CHA0: results field and pot experiments pp. 17-22. In: Keel, C., Koller, B. and Defago, G. (eds.). Plant Growth Promoting rhizobacteria. The second international workshop on plant growth promoting rhizobacteria. Interlacen, Switzerland. Phytopatholgy 68:1377-1383.
Yan Z, Reddy MS, Kloepper JW (2003) Survival and colonization of rhizobacteria in a tomato transplant system. Canadian Journal of Microbiology 49: 383-389. | ||
|
آمار تعداد مشاهده مقاله: 6,286 تعداد دریافت فایل اصل مقاله: 3,880 |
||