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معرفی پروتکل تجاری به منظور ازدیاد درون شیشه‌ای کلم زینتی Brassica oleracea var. acephala | ||
| علوم باغبانی ایران | ||
| مقاله 15، دوره 45، شماره 4، بهمن 1393، صفحه 475-484 اصل مقاله (428.01 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22059/ijhs.2015.53501 | ||
| نویسندگان | ||
| مینا تقی زاده* ؛ موسی سلگی | ||
| استادیار دانشکده کشاورزی ومنابع طبیعی دانشگاه اراک | ||
| چکیده | ||
| طی سالیان اخیر کلم زینتی به‌دلیل داشتن اندامهای هوایی رنگی بادوام و مقاومت خوب به شرایط محیطی در فضای سبز بسیار استفاده می‌شود. بنابراین، ازدیاد درون‌شیشه‌ای کلم زینتی در چند آزمایش مطالعه شد. غلظتهای مختلف بنزیل آدنین (mg/L1/0، 5/0 و 1)، 2,4-D (mg/L1/0، 5/0)، نیترات نقره (mg/L 2و4) و نوع رقم (بنفش و سفید) در مرحلۀ استقرار بهکار گرفته شد. همچنین تیمارهای مرحلۀ پرآوری شامل بنزیل آدنین (mg/L1/0 و 5/0) و ایندول بوتیریک اسید (mg/L1/0 و 5/0) بود. سازگاری گیاهچههای درون‌شیشه‌ای در مرحلۀ سازگاری با استفاده از غلظتهای مختلف MS و عصارۀ ورمی کمپوست (کامل و یک دوم) اجرا شد. نتایج نشان داد کاربرد mg/L1/0 بنزیل آدنین به همراه mg/ L 1 2,4-D باززایی قابل قبولی را در این گیاه زینتی فراهم می‌آورد. محیط کشت پرآوری تکمیل‌شده با mg/L 5/0 از بنزیل آدنین و ایندول بوتیریک اسید سبب توسعۀ شاخسارهها شد. سرانجام گیاهچههای درون‌شیشه‌ای با انتقال به محیط کشت MS تقلیل‌یافته به‌طور موفقیت‌آمیزی سازگار شدند. به‌طور‌کلی، در این پژوهش با استفاده از محیط کشت نسبتاً کم‌هزینه، در دسترس و حداقل غلظت تنظیم‌کننده‌های باززایی موفقیت‌آمیز کلم زینتی به دست آمد. | ||
| کلیدواژه‌ها | ||
| : باززایی درون‌شیشه‌ای؛ تنظیم‌کننده‌های رشد؛ کلم زینتی | ||
| عنوان مقاله [English] | ||
| Introduction of commercial protocol for in vitro proliferation of Brassica oleracea var. acephala | ||
| نویسندگان [English] | ||
| Mina Taghizadeh؛ Mousa Solgi | ||
| چکیده [English] | ||
| In recent years, ornamental kale is commonly used in landscapes due to its colorful shoots and resistance to the diverce environmental conditions. Thus, its in vitro proliferation was studied in series of experiments. Different BA (0/10.1, 0.5 and 1 mg/L), 2,4-D (0.1, 0.5 mg/L) and silver nitrate (2, 4 mg/L) treatments were applied for the establishment stage. Treatments of the proliferation stage also included BA (0.1 and 0.5 mg/L) and IBA (0.1 and 0.5 mg/L). Acclimatization of in vitro plantlets was conducted using different concentrations of MS (complete and half strength) and vermicompost extract (direct and diluted). Results revealed that, using of 0.1 mg/L BA with one mg/L 2,4-D was able to provide regeneration in this ornamental plant, acceptably. Proliferation medium supplemented with 0.5 and 0.5 mg/L BA caused shoot development. Finally, in vitro plantlets were successfully adapted by transferring to media containing reduced MS medium without plant growth regulators. In conclusion, by using the relatively low cost, accessible media and the least concentrations of plant growth regulators, successful regeneration of kale ornamental were obtained. | ||
| کلیدواژه‌ها [English] | ||
| Kale ornamental, in vitro regeneration, plant growth regulators | ||
| مراجع | ||
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