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ارتباط مجتمع عمده پذیرش بافتی طیور با پاسخ پادتن به واکسن | ||
| مجله تحقیقات دامپزشکی (Journal of Veterinary Research) | ||
| مقاله 7، دوره 70، شماره 2، تیر 1394، صفحه 163-170 اصل مقاله (809.92 K) | ||
| شناسه دیجیتال (DOI): 10.22059/jvr.2015.53733 | ||
| نویسندگان | ||
| غلامرضا نیکبخت بروجنی* 1؛ عاطفه اسماعیل نژاد2؛ ندا خازنی اسکوئی1 | ||
| 1گروه میکروبیولوژی و ایمونولوژی، دانشکده دامپزشکی دانشگاه تهران، تهران-ایران | ||
| 2گروه پاتوبیولوژی، دانشکده دامپزشکی دانشگاه شیراز، شیراز-ایران | ||
| چکیده | ||
| زمینه مطالعه: مجتمع عمده پذیرش بافتی (MHC) نقشی مرکزی در چگونگی پاسخ به بیماریهای عفونی دارد و پاسخ‌های ایمنی تحت کنترل ژن‌های این مجتمع هستند. بدلیل تنوع MHC تفاوت‌های فردی در پاسخ به واکسن‌های مختلف طیور مشاهده می‌شود. بررسی ارتباط آلل‌های MHC طیور با توان پاسخ به واکسن‌های معمول، به توفیق واکسیناسیون و کنترل بیماریها کمک خواهد نمود. هدف: هدف از مطالعه حاضر بررسی تنوع MHC در جمعیت طیور بومی خراسان و ارتباط آن با پاسخ پادتن به واکسن‌های گامبورو، نیوکاسل و آنفلوانزا است. روش‌کار: به منظور تعیین ژنوتیپ‌های MHC از نشانگر ریزماهواره LEI0258 و روش تحلیل قطعه‌ای استفاده شد. عیار پادتن تولید شده بر ضد واکسن‌های نیوکاسل و آنفلوانزا با آزمون ممانعت هماگلوتیناسیون و واکسن گامبورو با آزمون الایزا اندازه‌گیری شدند. تحلیل آماری با کمک نرم‌افزار SPSS نسخه 21 صورت پذیرفت. جهت تعیین ضرایب تأثیر آلل‌ها از تحلیل رگرسیون تک متغیره با روش حداقل مربعات استفاده شد. نتایج: در جمعیت طیور بومی خراسان 13 آلل ریزماهواره LEI0258 شناسایی شد که نشان‌دهنده تنوع بالای MHC در این جمعیت است. آلل 361 بیشترین (48/28%) و آلل 350 کمترین (69/0%) فراوانی را در جمعیت داشتند. در بررسی ارتباط MHC با پاسخ ایمنی، آلل 266 ریزماهواره LEI0258 با کاهش عیار پادتن ضد واکسن گامبورو و آلل‌های 311 و 313 با افزایش پاسخ پادتن به واکسن نیوکاسل همراه بودند (05/0<p). نتیجه‌گیری‌نهایی: با توجه به نقش قابل ملاحظه MHC در مقاومت یا حساسیت در برابر بیماریهای عفونی ویروسی و کیفیت پاسخ‌های ایمنی، از روش‌ها و نتایج بدست آمده می‌توان برای انتخاب و بهبود جمعیت‌های در حال اصلاح نژاد بهره برد. | ||
| کلیدواژه‌ها | ||
| گامبورو؛ آنفلوانزا؛ مجتمع عمده پذیرش بافتی؛ ریزماهواره؛ نیوکاسل | ||
| عنوان مقاله [English] | ||
| Study of the association of major histocompatibility complex with antibody response to vaccines in Khorasan native chickens | ||
| نویسندگان [English] | ||
| Gholamraza Nikbakhat Brujeni1؛ Atefeh Esmailnejad2؛ Neda Khazeni Oskoui1 | ||
| 1Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran-Iran | ||
| 2Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz-Iran | ||
| چکیده [English] | ||
| BACKGROUND: Major histocompatibility complex (MHC) plays a central role in regulation and control of the immune responses to infectious diseases. Due to its polymorphism, individual differences in response to vaccines have been observed in different chicken populations. Studying the association of chicken MHC with immune response to vaccines will help the control of infectious disease and vaccination success. Objectives: The present study aimed to evaluate the MHC polymorphism and its association with antibody response against infectious bursal disease (Gumboro), Newcastle (ND) and Influenza (AI) vaccines in Khorasan native chickens. Methods: Diversity of LEI0258 microsatellite marker (MHC genotyping) was investigated by fragment analysis method. Antibody titer against IBD was measured by ELISA and antibody titers against ND and AI vaccines were measured by Haemaglutination Inhibition (HI) assay. Statistical analysis was performed using SPSS software (version 21). Univariate regression analysis was performed using weighted least squares with weight number of progeny mean data. Results: Total of 13 LEI0258 microsatellite alleles were identified in Khorasan native chickens which indicated a high genetic diversity in the population. The allele 361 bp had the highest (28.48%) and the allele 350 bp had the lowest (0.69%) frequency, respectively. In evaluating the association of MHC with immune responses, 311 and 313 bp alleles were significantly associated with elevated immune responses to Newcastle vaccine, while allele 266 bp was associated with lower IBDV antibody titers (p<0.05). ConclusionS: According to the important role of MHC in controlling infectious disease resistance or susceptibility and quality of immune responses, these results could be used for selection and improving the populations under selective breeding. | ||
| کلیدواژه‌ها [English] | ||
| Gumboro, influenza, major histocompatibility complex, Microsatellite, newcastle | ||
| مراجع | ||
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