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Antifungal activity of recombinant rice LTP2 on some phytopathogenic fungi | ||
| Progress in Biological Sciences | ||
| مقاله 12، دوره 5، شماره 1، شهریور 2015، صفحه 133-142 اصل مقاله (914.42 K) | ||
| نوع مقاله: Original Research Papers | ||
| شناسه دیجیتال (DOI): 10.22059/pbs.2015.53976 | ||
| نویسندگان | ||
| Fatemeh Zebardast1؛ Mohammadreza Zamani* 2؛ Mostafa Motallebi2؛ Zahra Moghaddassi-Jahromi2 | ||
| 1National Institute of Genetic Engineering and Biotechnology, Tehran, Iran | ||
| 2National Institute of Genetic Engineering and Biotechnology | ||
| چکیده | ||
| Plant lipid transfer proteins (LTPs) are members of the pathogenesis-related proteins (PR-14) and some of them exhibit activity against phytopathogenic fungi. To investigate whether rice LTP2 plays a role in antifungal activity, the coding region of an Iranian rice Ltp2 gene was cloned into expression vector pET24-d(+) and then expressed in Escherichia coli Rosetta strain (DE3). The potential antifungal activity of the purified LTP2 was tested on mycelial growth of some important phytopathogenic fungi. The results showed that the rice LTP2 inhibit the growth of Rhizoctonia solani, Botrytis cinerea,Sclerotinia sclerotiorum, Fusarium sporotrichioides and F. oxysporum. Also, the purified LTP2 protein was shown to strongly inhibit spore germination and consequential mycelia of Alternaria brassicola. | ||
| کلیدواژهها | ||
| antifungal activity؛ lipid transfer protein 2؛ phytopathogenic fungi؛ prokaryotic expression | ||
| مراجع | ||
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