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Ashrfai, Mohsen, Farsi, Mohammad, Mirshamsi, Amin, Parvandi, Mozhgan. (1394). Isolation and Sequence Analysis of GpdII Promoter of the White Button Mushroom (Agaricus bisporus) from Strains Holland737 and IM008. , 2(1), 33-41. doi: 10.22059/ijhst.2015.54262
Mohsen Ashrfai; Mohammad Farsi; Amin Mirshamsi; Mozhgan Parvandi. "Isolation and Sequence Analysis of GpdII Promoter of the White Button Mushroom (Agaricus bisporus) from Strains Holland737 and IM008". , 2, 1, 1394, 33-41. doi: 10.22059/ijhst.2015.54262
Ashrfai, Mohsen, Farsi, Mohammad, Mirshamsi, Amin, Parvandi, Mozhgan. (1394). 'Isolation and Sequence Analysis of GpdII Promoter of the White Button Mushroom (Agaricus bisporus) from Strains Holland737 and IM008', , 2(1), pp. 33-41. doi: 10.22059/ijhst.2015.54262
Ashrfai, Mohsen, Farsi, Mohammad, Mirshamsi, Amin, Parvandi, Mozhgan. Isolation and Sequence Analysis of GpdII Promoter of the White Button Mushroom (Agaricus bisporus) from Strains Holland737 and IM008. , 1394; 2(1): 33-41. doi: 10.22059/ijhst.2015.54262

Isolation and Sequence Analysis of GpdII Promoter of the White Button Mushroom (Agaricus bisporus) from Strains Holland737 and IM008

International Journal of Horticultural Science and Technology
مقاله 4، دوره 2، شماره 1، شهریور 2015، صفحه 33-41    اصل مقاله (456.1 K)
نوع مقاله: Research paper
شناسه دیجیتال (DOI): 10.22059/ijhst.2015.54262
نویسندگان
Mohsen Ashrfai* 1؛ Mohammad Farsi2؛ Amin Mirshamsi2؛ Mozhgan Parvandi2
1Department of Agronomy and Plant Breeding, College of Agriculture, University of Zanjan, Zanjan, Iran
2Department of Plant Breeding and Plant Biotechnology, College of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran
چکیده
Many recent studies have shown that glycosylation patterns of Agaricus bisporus are similar to those of mammalians, so that this organism is a good candidate for the expression of glycosylated pharmaceutical protein. To achieve constant interested gene expression in all cells of the organism, proper promoter isolation is necessary. To isolate this promoter, PCR with specific primers was performed on extracted DNA of the white button mushroom strains Holland737 and IM008. The PCR amplified 290 bp fragments of gpdII promoters. IM008 gpdII promoter was used to construct pCAMBIAH8 plasmid. Comparison of isolated promoters among sequence records at NCBI demonstrated high similarity between IM008 gpdII promoter and previously reported gpdII promoter. Sequence analysis of isolated promoters revealed several point mutations on this promoter. TACAAA promoter sequence in −65 site acts as TATA box. Among the three CAAT candidate sequences, one is functional, which is located at position −108. Transformation of the white button mushroom with constructed pCAMBIAH8 plasmid was successfully performed.
کلیدواژه‌ها
CAAT box؛ Pharmaceutical protein؛ TATA box؛ Transgenic mushroom
مراجع
  1. Bitter, G.A., and K.M. Egan. 1984. Expression of Heterologous Genes In Saccharomyces Cerevisiae from Vectors Utilizing the Glyceraldehyde-3-Phosphate Dehydrogenase Gene Promoter. Gene 32:263–74.
  2. Burns, C., K. E. Gregory, M. Kirby, M.K. Cheung, M. Riquelme, T.J. Elliott, M. P. Challen, A. Bailey, and G.D. Foster. 2005. Efficient GFP Expression in the Mushrooms Agaricus Bisporus and Coprinus Cinereus Requires Introns. Mol. Biotechnol. 42:191–99.
  3. Butler, J.E.F, and J.T. Kadonaga. 2002. The RNA Polymerase II Core Promoter: A Key Component in the Regulation of Gene Expression. Gene. Dev. 16:2583–2592.
  4. Chen, X., M. Stone, C. Schlagnhaufer, and C.P. Romaine. 2000. A Fruiting Body Tissue Method for Efficient Agrobacterium-Mediated Transformation of Agaricus Bisporus. Appl. Environ. Microb. 66:4510–13.
  5. Deng, W., and S.G.E. Roberts. 2005. A Core Promoter Element Downstream of the TATA Box That Is Recognized by TFIIB. Gene. Dev. 19:2418–2423.
  6. Döring, F., M. Klapper, S. Theis, and H. Daniel. 1998. Use of the Glyceraldehyde-3-Phosphate Dehydrogenase Promoter for Production of Functional Mammalian Membrane Transport Proteins in the Yeast Pichia Pastoris. Biochem. Biophys. Res. Commun. 250:531–35.
  7. Harmsen, M.C., F.H.J Schuren, S.M. Moukha, C.M. van Zuilen, P.J. Punt, and J.G.H. Wessels. 1992. Sequence Analysis of the Glyceraldehyde-3-Phosphate Dehydrogenase Genes from the Basidiomycetes Schizophyllum Commune, Phanerochaete Chrysosporium and Agaricus Bisporus. Curr. Genet. 22:447–454.
  8. Hirano, T., T. Sato, K. Yaegashi, and H. Enei. 2000. Efficient Transformation of the Edible Basidiomycete Lentinus Edodes with a Vector Using a Glyceraldehyde-3-Phosphate Dehydrogenase Promoter to Hygromycin B Resistance. M. G. G. 263:1047–52.
  9. Kato, M. 2005. An Overview of the CCAAT-Box Binding Factor in Filamentous Fungi: Assembly, Nuclear Translocation, and Transcriptional Enhancement. Biosci. Biotechnol. Biochem. 69:663–72.

10. Kilaru, S., and U. Kues. 2005. Comparison of gpd Genes and Their Protein Products in Basidiomycetes. Fungal. Genet. Newsl. 52:1-23.

11. Kuo, C.Y., S.Y. Chou, and C.T. Huang. 2004. Cloning of Glyceraldehyde-3-Phosphate DehydrogenaseGene and Use of the Gpd Promoter for Transformation in Flammulina velutipes. Appl. Microbiol. Biot. 65:593–599.

12. Molloy, S. 2004. Sugar Transport and Water Relations of Agaricus bisporus. PhD Diss. Univ. of Bedford, Cranfield, Institute of Bioscience and Technology.

13. Nazrul, M.I., and B.Y. Bing. 2010. ISSR as New Markers for Identification of Homokaryotic Protoclones of Agaricus bisporus. Curr. Microbiol. 60:92–98.

14. Piechaczyk, M., J. M. Blanchard, L. Marty, C. Dani, F. Panabieres, S. E Sabouty, P. Fort, and P. Jeanteur. 1984. Post-Transcriptional Regulation of Glyceraldehyde-3-Phosphate-Dehydrogenase Gene Expression in Rat Tissues. Nucleic. Acids Res. 12:6951–6963.

15. Prize, B. and C. Lang. 2004. Genetics and Biotechnology, p. 130. U. Kück (eds.) Gene Regulation in Yeast. Springer Science & Business Media, Berlin.

16. Punt, P.J., M.A. Dingemanse, A. Kuyvenhoven, R.D.M. Soede, P.H. Pouwels, and C.A.M.J.J. van den Hondel. 1990. Functional Elements in
the Promoter Region of The Aspergillus nidulans gpdA Gene Encoding Glyceraldehyde-3-Phosphate Dehydrogenase. Gene 93:101–109.

17. Romaine, C. P., and C. Schlagnhaufer. 2007. Mushroom (Agaricus bisporus). Methods. Mol. Biol. In Agrobacterium Protocols. 2:453–63.

18. Sambrook, J., and D.W. Russell. (ed.) 2001. Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, 2344 p.

19. Smale, S.T., and J.T. Kadonaga. 2003. The RNA Polymerase II Core Promoter. Annu. Rev. Biochem. 72:449–79.

20. Sonnenberg, A. S. M., and L.J.L.D. van Griensven. 2000. Genetics and Breeding of Agaricus bisporus. In Science and Cultivation of Edible Fungi. Congr. on the Science and Cultivation of Edible Fungi, May15-19, 2000. pp. 25–39.

21. Upshall, A., A.A. Kumar, M.C. Bailey, M.D. Parker, M.A. Favreau, K.P. Lewison, M.L. Joseph, J.M. Maraganore, and G.L. McKnight. 1987. Secretion of Active Human Tissue Plasminogen Activator from the Filamentous Fungus Aspergillus nidulans. Nat. Biotechnol. 5:1301–1304.

22. Velcko, A.J., R.W. Kerrigan, L.A. MacDonald, M.P. Wach, C. Schlagnhaufer, and C.P. Romaine. 2004. Expression of Novel Genes in Agaricus bisporus Using an Agrobacterium-Mediated Transformation Technique. I.S.M.S. 16:591–598.

23. Wolff, A.M., and J. Arnau. 2002. Cloning of Glyceraldehyde-3-Phosphate Dehydrogenase-Encoding Genes in Mucor circinelloides (Syn. Racemosus) and Use of the gpd1 Promoter for Recombinant Protein Production. Fungal. Genet. Biol. 35:21–29.

24. Zhang, C., V. Odon, H.K. Kim, M.P. Challen, K.S. Burton, D. Hartley, and T.J. Elliott. 2004. Mushrooms for Molecular Pharming. I.S.M.S. 16:611–618.

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