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Ashrfai, Mohsen, Farsi, Mohammad, Mirshamsi, Amin, Parvandi, Mozhgan. (1394). Isolation and Sequence Analysis of GpdII Promoter of the White Button Mushroom (Agaricus bisporus) from Strains Holland737 and IM008. , 2(1), 33-41. doi: 10.22059/ijhst.2015.54262
Mohsen Ashrfai; Mohammad Farsi; Amin Mirshamsi; Mozhgan Parvandi. "Isolation and Sequence Analysis of GpdII Promoter of the White Button Mushroom (Agaricus bisporus) from Strains Holland737 and IM008". , 2, 1, 1394, 33-41. doi: 10.22059/ijhst.2015.54262
Ashrfai, Mohsen, Farsi, Mohammad, Mirshamsi, Amin, Parvandi, Mozhgan. (1394). 'Isolation and Sequence Analysis of GpdII Promoter of the White Button Mushroom (Agaricus bisporus) from Strains Holland737 and IM008', , 2(1), pp. 33-41. doi: 10.22059/ijhst.2015.54262
Ashrfai, Mohsen, Farsi, Mohammad, Mirshamsi, Amin, Parvandi, Mozhgan. Isolation and Sequence Analysis of GpdII Promoter of the White Button Mushroom (Agaricus bisporus) from Strains Holland737 and IM008. , 1394; 2(1): 33-41. doi: 10.22059/ijhst.2015.54262

Isolation and Sequence Analysis of GpdII Promoter of the White Button Mushroom (Agaricus bisporus) from Strains Holland737 and IM008

International Journal of Horticultural Science and Technology
مقاله 4، دوره 2، شماره 1، شهریور 2015، صفحه 33-41    اصل مقاله (456.1 K)
نوع مقاله: Research paper
شناسه دیجیتال (DOI): 10.22059/ijhst.2015.54262
نویسندگان
Mohsen Ashrfai* 1؛ Mohammad Farsi2؛ Amin Mirshamsi2؛ Mozhgan Parvandi2
1Department of Agronomy and Plant Breeding, College of Agriculture, University of Zanjan, Zanjan, Iran
2Department of Plant Breeding and Plant Biotechnology, College of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran
چکیده
Many recent studies have shown that glycosylation patterns of Agaricus bisporus are similar to those of mammalians, so that this organism is a good candidate for the expression of glycosylated pharmaceutical protein. To achieve constant interested gene expression in all cells of the organism, proper promoter isolation is necessary. To isolate this promoter, PCR with specific primers was performed on extracted DNA of the white button mushroom strains Holland737 and IM008. The PCR amplified 290 bp fragments of gpdII promoters. IM008 gpdII promoter was used to construct pCAMBIAH8 plasmid. Comparison of isolated promoters among sequence records at NCBI demonstrated high similarity between IM008 gpdII promoter and previously reported gpdII promoter. Sequence analysis of isolated promoters revealed several point mutations on this promoter. TACAAA promoter sequence in −65 site acts as TATA box. Among the three CAAT candidate sequences, one is functional, which is located at position −108. Transformation of the white button mushroom with constructed pCAMBIAH8 plasmid was successfully performed.
کلیدواژه‌ها
CAAT box؛ Pharmaceutical protein؛ TATA box؛ Transgenic mushroom
مراجع
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