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ریزازدیادی ختمی چینی در شرایط درون شیشه ای | ||
به نژادی گیاهان زراعی و باغی | ||
مقاله 5، دوره 3، شماره 2، آذر 1394، صفحه 191-200 اصل مقاله (3.47 M) | ||
نوع مقاله: مقاله پژوهشی | ||
نویسندگان | ||
فاطمه فیضی1؛ موسی موسوی* 2؛ مهر انگیز چهرازی2 | ||
1کارشناس ارشد باغبانی (گل و گیاهان زینتی) گروه علوم باغبانی، دانشکده کشاورزی، دانشگاه شهید چمران اهواز | ||
2استادیار گروه علوم باغبانی، دانشکده کشاورزی، دانشگاه شهید چمران اهواز | ||
چکیده | ||
به منظور بررسی ریزازدیادی ختمی چینی در شرایط درونشیشهای، سه آزمایش به صورت طرح کاملاً تصادفی با 10 تکرار، در آزمایشگاه کشت بافت دانشکده کشاورزی دانشگاه شهید چمران اهواز در سال 1393 انجام گرفت. در آزمایش اول محیط پایه مناسب مشخص شد. آزمایش دوم بهترین غلظت هورمون BAP جهت القای شاخه و آزمایش سوم بهترین نوع و غلظت هورمون ریشهزایی مشخص شد. مقایسه میانگین آزمایش اول نشان داد که بیشترین میانگین طول شاخساره، تعداد برگ، وزن تر و وزن خشک شاخساره در محیط VS نسبت به سایر محیطها مشاهده شد. مقایسه میانگین آزمایش دوم نشان داد که غلظت 5/0 میلیگرم بر لیتر هورمون BAP بیشترین میانگین طول شاخساره، تعداد برگ، وزن تر و وزن خشک شاخساره نسبت به سایر غلظتها را داشت. مقایسه میانگین آزمایش سوم نشان داد که غلظت 2/0 میلیگرم در لیتر هورمون IBAبیشترین میانگین درصد ریشهزایی، تعداد ریشه و طول ریشه نسبت به سایر غلظتها را داشت. | ||
کلیدواژهها | ||
بنزیل آمینوپورین؛ پزآوری؛ ختمی چینی؛ ریشه زایی؛ محیط VS | ||
عنوان مقاله [English] | ||
Micropropagation of Hibiscus rosa-sinensis through tissue culture | ||
نویسندگان [English] | ||
Fatemeh Feizi1؛ Mousa Mousavi2؛ Mehrangiz Chehrazi2 | ||
1Graduate M.Sc. Student, Department of Horticulture, Faculty of Agriculture, Shahid Chamran University of Ahvaz. Ahvaz | ||
2Assistant Professor, Department of Horticulture, Faculty of Agriculture, Shahid Chamran University of Ahvaz, Ahvaz | ||
چکیده [English] | ||
In order to investigate the in vitro micropropagation Hibiscus Rosa-sinensis three tests in a completely randomized design with 10 replications were performed in tissue culture laboratory Shahid Chamran University in 2014.The first experiment showed the perfect media.Second tests best consentration BAP for multiplication and third tests showed the best type and concentration rooting hormone. Compare average of first experiments showedthe highest average shoot length, number of leaves, fresh weight and dry weight was observed in VS than other media. Compare average of second experiments showedthe concentration of 5.0 mg per liter hormone BAP highest average shoot length, number of leaves, fresh weight and dry weight than other concentrations was observed.Compare third experiment showed that the average concentration of 2.0 mg per liter of IBA hormone highest percentage of rooting, number of roots and root length was compared to other concentrations. | ||
کلیدواژهها [English] | ||
BAP, Hibiscus rosa-sinensis, Media VS, proliferation, Rotting | ||
مراجع | ||
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