تعداد نشریات | 161 |
تعداد شمارهها | 6,532 |
تعداد مقالات | 70,502 |
تعداد مشاهده مقاله | 124,118,886 |
تعداد دریافت فایل اصل مقاله | 97,225,012 |
بررسی امکان کنترل بیماری بلاست مرکبات با استفاده از عصاره بذر گلپر و مخمرهای رورست | ||
کنترل بیولوژیک آفات و بیماری های گیاهی | ||
مقاله 5، دوره 4، شماره 2، مهر 1394، صفحه 109-119 اصل مقاله (568.2 K) | ||
نوع مقاله: مقاله پژوهشی | ||
شناسه دیجیتال (DOI): 10.22059/jbioc.2015.57172 | ||
نویسندگان | ||
محمد ادبی فیروزجایی1؛ پژمان خدایگان* 2؛ فرید بیگی3؛ روح الله صابری ریسه2 | ||
1کارشناس ارشد، گروه گیاهپزشکی، دانشکدة کشاورزی، دانشگاه ولیعصر (عج) رفسنجان | ||
2دانشیار، گروه گیاهپزشکی، دانشکدة کشاورزی، دانشگاه ولیعصر (عج) رفسنجان | ||
3استادیار مؤسسة تحقیقات گیاهپزشکی کشور، سازمان تحقیقات، آموزش و ترویج کشاورزی، تهران، ایران | ||
چکیده | ||
بلاست مرکبات یکی از بیماریهای مهم مرکبات در شمال کشور است که توسط گونههایی از جنس سودوموناس (Pseudomonas spp.) ایجاد میشود. این بیماری در شرایط اقلیمی مساعد، خسارتهای چشمگیری به باغهای مرکبات وارد میکند. با توجه به محدودیتهای استفاده از آنتیبیوتیکها و ترکیبات شیمیایی سنتزی، کنترل بیولوژیک یا استفاده از ترکیبات گیاهی ضد میکروبی میتواند روشی مؤثر برای کنترل بیماری بلاست باشد. در این تحقیق اثر چند جدایة مخمر رورست و عصارة بذر گلپر ایرانی (Heracleum persicum) بر عوامل بلاست مرکبات در شرایط آزمایشگاه (آزمون هالة بازدارندگی) و گلخانه، بررسی شد. جدایههای آنتاگونیست از نمونههای برگ مرکبات مناطق مختلف استانهای شمالی کشور جداسازی شدند. همچنین، بذر گلپر از مناطق مرتفع شهرستان بابل جمعآوری شد. در بررسی آزمایشگاهی، عصارة گلپر اثر معنیداری در مهار رشدی دو عامل بیمارگر از خود نشان داد، اما هیچیک از جدایههای مخمری نتوانستند بازدارندگی علیه دو بیمارگر ایجاد کنند. نتایج بررسی گلخانهای نشان داد که یکی از جدایههای مخمری (Pichia sp.) با میانگین کاهش 8/78 درصدی بهترین جدایه در کاهش شدت بیماری بلاست مرکبات بود. همچنین، عصارة گلپر توانست جمعیت بیمارگرها را در سطح میوة مرکبات، نسبت به شاهد تا 60 درصد کاهش دهد. یافتههای این تحقیق نشان داد که استرینPichia sp. و عصارة گلپر در کنترل بیماری مؤثر هستند. | ||
کلیدواژهها | ||
بلاست مرکبات؛ عصارة گلپر؛ کنترل بیولوژیک؛ مخمر؛ Pichia sp | ||
عنوان مقاله [English] | ||
Investigation on control of citrus blast disease by seed extract of Heracleum persicum (Golpar) and epiphytic yeasts | ||
نویسندگان [English] | ||
Mohammad Adabi Firoozjaee1؛ Pejman Khodaygan2؛ Farid Beiki3؛ Roohallah Saberi-Riseh2 | ||
1Former M. Sc. Student, Department of Plant Protection, College of Agriculture, Vali-e-Asr Univrsity of Rafsanjan, Iran | ||
2Associate Professor, Former M. Sc. Student Department of Plant Protection, College of Agriculture, Vali-e-Asr Univrsity of Rafsanjan, Iran | ||
3Assistant Professor, Plant Pathology Research Institute, Tehran, Iran | ||
چکیده [English] | ||
Citrus blast caused by species of Pseudomonas spp. is one of the most important citrus diseases in north of Iran. This disease causes considerable losses to citrus orchards in conducive climatic conditions. Due to restrictions on the use of antibiotics and chemical compounds, biological control and use of plant antimicrobial compounds can be an effective way to control citrus blast disease. In this study, performance of several epiphytic yeasts isolates and Golpar seed extract on bacterial blast of citrus were studied under in vivo and in vitro conditions. Antagonistic yeasts collected from citrus leaf samples were isolated from different regions of the Northern provinces. Also, Golpar seeds were collected from the highlands of the city of Babol (Mazandaran, Iran). The seed extract of Golpar showed significantly inhibited the growth of two pathogens on KB medium but none of the yeasts show significant growth inhibition. The results of greenhouse studies indicated that one of yeast isolate (Pichia sp.) reduces the severity of disease with average 78.8 percent. Also Golpar extract was able to reduce the pathogen populations by 60 percent in citrus fruit. The results showed that Pichia sp. and Golpar extract are effective for controlling the disease. | ||
کلیدواژهها [English] | ||
Biocontrol, citrus blast, golpar extract, Pichia sp, Yeast | ||
مراجع | ||
Ahmadian-Attari M, Esfahani HM, Amin G, Fazeli M, Jamalifar H, Kamalinia G, Khanlarbeik M, Ashtiani H, Farsam H (2009) The ethnopharmacological study on antibacterial activity of some selected plants used in Iranian traditional medicine. Journal of Medicinal Plants, 8: 50-57. Arras G, De Cicco V, Arru S, Lima G (1998) Biocontrol by yeasts of blue mould of citrus fruits and the mode of action of an isolate of Pichia guilliermondii. Journal of horticultural science & biotechnology, 73: 413-418. Arras G, Scherm B, Migheli Q (2002) Improving biocontrol activity of Pichia guillermondii against post-harvest decay of oranges in commercial packing-houses by reduced concentrations of fungicides. Biocontrol Science and Technology, 12: 547-553. Balestra G, Varvaro L (1995) Epiphytic survival and control of Pseudomonas viridiflava on Actinidia deliciosa. III International Symposium on Kiwifruit 4441995. 745-750. Beiki F, Gholtapeh EM, Rahimian H, Shamsbakhsh M, Barzegar A, Bisbal AB, Lalucat J (2012) Biological control of citrus blast disease using some yeast strains isolated from citrus orchards in the northern provinces of Iran. Biocontrol in Plant Protection, 1: 43-64. (In Persian). Brandl M (2008) Plant lesions promote the rapid multiplication of Escherichia coli O157: H7 on postharvest lettuce. Applied and Environmental Microbiology, 74: 5285-5289. Bryson V, Szybalski W (1952) Microbial selection. Science (New York, NY), 116: 45-51. Buck JW, Andrews JH (1999) Attachment of the yeast Rhodosporidium toruloides is mediated by adhesives localized at sites of bud cell development. Applied and Environmental microbiology, 65: 465-471. Campbell CL, Madden LV (1990) Introduction to plant disease epidemiology. John Wiley & Sons. Chalutz E, Wilson C (1990) Postharvest biocontrol of green and blue mold and sour rot of citrus fruit by Debaryomyces hansenii. Plant Disease, 74: 134-137. Coit J (1916) Citrus blast: a new disease in California. Calif. Univ. Jotlr. Agriculture 3: 234-235. Deak T (2007) Handbook of food spoilage yeasts, Second Edition. CRC Press. Droby S, Bar-Shimon M, Yehuda H, Cohen L, Daus A, Goldway M, Wisniewski M (2004) Involvement of lytic enzymes in the mode of action of the yeast Candida Oleophila used to control postharvest diseases. International Society for Horticultural Science Meeting 2004. Droby S, Chalutz E, Wilson C, Wisniewski M (1989) Characterization of the biocontrol activity of Debaryomyces hansenii in the control of Penicillium digitatum on grapefruit. Canadian Journal of Microbiology, 35: 794-800. Dutova T, Tolstorukov I (1997) Genetic control of purine biosynthesis in the yeast Pichia methanolica." Roman's effect" in red adenine-dependent pur6 and pur7 mutants. Genetika, 33: 1345-1353. El-Ghaouth A, Wilson C, Wisniewski M (2001) Induction of systemic resistance in apple by the yeast antagonist Candida saitoana. IOBC WPRS Bulletin 24: 309-312. El-Neshawy S, El-Sheikh-Aly M (1998) Control of green mould on oranges by Candida oleophila and calcium treatments. Annals of Agricultural Science (Egypt). Everett K, Vanneste J, Hallett I, Walter M (2005) Ecological alternatives for disease management of fruit rot pathogens. New Zealand Plant Protection, 58: 55-61. Ferguson L, Grafton-Cardwell EE (2014) Citrus production manual. UCANR Publications. Hemati A, Azarnia M, Angaji A (2010) Medicinal effects of Heracleum persicum (Golpar). Middle-East J. Sci. Res, 5: 174-176. Iscan G, Demirci F, Kurkcuoglu M, Kivanc M, Baser KH (2003) The bioactive essential oil of Heracleum sphondylium L. subsp. ternatum (Velen.) Brummitt. Z Naturforsch C, 58: 195-200. Kousha A, Bayat M (2012) Bactericidal and fungicidal activity of methanolic extracts of Heracleum persicum Desf. ex Fischer against some aquatic and terrestrial animal pathogens. International Journal of Pharmacology, 8. Kurtzman CP, Fell JW (1998) The yeast: A taxonomic study, Amsterdam. Kurtzman C, Fell JW, Boekhout T (2011) The Yeasts: A Taxonomic Study. Elsevier Science. Lahlali R, Serrhini MN, Jijakli MH (2004) Efficacy assessment of Candida oleophila (strain O) and Pichia anomala (strain K) against major postharvest diseases of citrus fruits in Morocco. Communications in Agricultural and Applied Biological Sciences. 69: 601-609. Long C-A, Deng B-X, Deng X-X (2007) Commercial testing of Kloeckera apiculata, isolate 34-9, for biological control of postharvest diseases of citrus fruit. Annals of microbiology, 57: 203-207. Marconi AM, Beltrametti F, Bestetti G, Solinas F, Ruzzi M, Galli E., Zennaro E (1996) Cloning and characterization of styrene catabolism genes from Pseudomonas fluorescens ST. Applied and Environmental Microbiology, 62: 121-127. Mercier J, Lindow S (2001) Field performance of antagonistic bacteria identified in a novel laboratory assay for biological control of fire blight of pear. Biological Control, 22: 66-71. Mercier J, Wilson C (1994) Colonization of apple wounds by naturally occurring microflora and introduced Candida oleophila and their effect on infection by Botrytis cinerea during storage. Biological Control, 4: 138-144. Marsit S, Mena A, Bigey F, Sauvage FX, Couloux A, Guy J, Legras JL, Barrio E, Dequin S, Galeote V (2015) Evolutionary Advantage Conferred by an Eukaryote-to-Eukaryote Gene Transfer Event in Wine Yeasts. Mol Biol Evol, 32: 1695-1707. Naatsaari L, Mistlberger B, Ruth C, Hajek T, Hartner FS, Glieder A (2012) Deletion of the Pichia pastoris KU70 homologue facilitates platform strain generation for gene expression and synthetic biology. PLoS One, 7: e39720. Nantawanit, N, Chanchaichaovivat, A, Panijpan, B. and Ruenwongsa, P. (2010) Induction of defense response against Colletotrichum capsici in chili fruit by the yeast Pichia guilliermondii strain R13. Biological Control, 52: 145-152. Noudeh GD, Sharififar F, Noodeh AD, Moshafi MH, Afzadi MA, Behravan E, Aref M, Sakhtianchi R (2010) Antitumor and antibacterial activity of four fractions from Heracleum persicum Desf. and Cinnamomum zeylanicum Blume. J Med Plants Res, 4: 2176-2180. Nunes CA (2012) Biological control of postharvest diseases of fruit. European Journal of Plant Pathology, 133: 181-196. Program UOCIPM (2012) Integrated Pest Management for Citrus. UCANR Publications. Qing F, Shiping T (2000) Postharvest biological control of Rhizopus rot of nectarine fruits by Pichia membranefaciens. Plant disease, 84: 1212-1216. Sakthivel N, Mew T (1991) Efficacy of bacteriocinogenic strains of Xanthomonas oryzae pv. oryzae on the incidence of bacterial blight disease of rice (Oryza sativa L.). Canadian Journal of Microbiology, 37: 764-768. Shams-Bakhsh M, Rahimian H (1997) Comparative study on agents of citrus blast and bacterial canker of stone fruits in Mazandaran. Iranian Journal of Plant Pathology, 33: 44-47. (in Persian) Sharififar F, Moshafi MH, Dehghan-Nudehe G, Ameri A, Alishahi F, Pourhemati A (2009) Bioassay screening of the essential oil and various extracts from 4 spices medicinal plants. Pak J Pharmcol Science 22: 317-322. Sharma R, Singh D, Singh R (2009) Biological control of postharvest diseases of fruits and vegetables by microbial antagonists: A review. Biological Control, 50: 205-221. Shulaev V, León J, Raskin I (1995) Is salicylic acid a translocated signal of systemic acquired resistance in tobacco? The Plant Cell Online, 7: 1691-1701. Suh S-O, Blackwell M, Kurtzman CP, Lachance M-A (2006) Phylogenetics of Saccharomycetales, the ascomycete yeasts. Mycologia, 98: 1006-1017. Wagih E (1991) Neither indole acetic acid nor bacteriocin is apparently involved in the in vitro antagonism between the virulent and the avirulent strains of Pseudomonas solanacearum. Journal of Phytopathology, 132: 153-160. Walsh PS, Metzger DA, Higuchi R (1991) Chelex 100 as a medium for simple extraction of DNA for PCR-based typing from forensic material. Biotechniques, 10: 506-513. Whiley AW, Schaffer BA, Wolstenholme BN (2002) The avocado: botany, production and uses. CABI. Whiteside JO, Garnsey SM, Timmer LW (1988) Compendium of citrus diseases. American Phytopathological Society. Wilson CL, Chalutz E (1989) Postharvest biological control of Penicillium rots of citrus with antagonistic yeasts and bacteria. Scientia Horticulturae, 40: 105-112. Yessad S, Manceau C, Luisetti J (1992) A detached leaf assay to evaluate virulence and pathogenicity of strains of Pseudomonas syringae pv. syringae on pear. Plant disease, 76: 370-373. | ||
آمار تعداد مشاهده مقاله: 6,461 تعداد دریافت فایل اصل مقاله: 1,538 |