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مطالعة تأثیر برخی شرایط محیطی و تغذیه‌ای بر تولید بیوفیلم استرین‌های باکتری پروبیوتیک Bacillus subtilis | ||
| کنترل بیولوژیک آفات و بیماری های گیاهی | ||
| مقاله 9، دوره 4، شماره 2، مهر 1394، صفحه 157-165 اصل مقاله (586.97 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22059/jbioc.2015.57176 | ||
| نویسنده | ||
| مریم خضری* | ||
| استادیار گروه گیاه‌پزشکی، دانشکدة کشاورزی، دانشگاه ارومیه | ||
| چکیده | ||
| تشکیل بیوفیلم در باکتری‌ها یک استراتژی مهم برای بقای آن‌هاست. بیوفیلم در باکتری پروبیوتیک Bacillus subtilisبه‌صورت زنجیرة طویلی از سلول‌هاست که با یک ماتریکس پلیمری از جنس پلی‌ساکاریدهای خارج سلولی احاطه شده است. این باکتری که یکی از عوامل مؤثر مهار زیستی بیمارگرهای گیاهی شناخته شده است، قابلیت زیادی در تولید بیوفیلم دارد. این مطالعه با هدف بررسی اثر شرایط محیطی شامل دما، اسیدیته و فشار اسمزی و همچنین، عناصر غذایی مانند قندها و اسیدآمینه‌های مترشحه از ریشة گندم و عناصر مهم خاک از قبیل کاتیون‌های کلسیم، منگنز، منیزیم، آهن، روی، مولیبدن، کبالت، بر و مس در تشکیل بیوفیلم این باکتری با استفاده از پلیت پلی‌استرن انجام شده است.براساسنتایج این تحقیق،بیشترین میزان بیوفیلم در سه استرین مورد مطالعه، در دمای 30 درجة سلسیوس، اسیدیتة 7 و فشار اسمزی حاصل از غلظت 75 درصد سوکروز تولید شد. عناصر منیزیم، کلسیم، روی، آهن، منگنز و مس موجب افزایش و عناصر کبالت، بر و مولیبدن موجب کاهش تولید بیوفیلم شدند. همة قندها و اسیدآمینه‌های مترشحه از ریشة گندم بر تولید بیوفیلم استرین‌های مورد مطالعه اثر افزایشی نشان دادند. بیشترین اثر افزایشی مربوط به قندهای آرابینوز و گلوکز و اسیدآمینه‌های لیزین و آسپارژین بود. براساس نتایج این پژوهش، شرایط محیطی و محتوای مواد غذایی می‌تواند بر تولید بیوفیلم و به تبع آن بقا و استقرار باکتری‌های پروبیوتیک در محیط و همچنین، کلنیزاسیون میزبان و توان مهار زیستی عوامل بیماریزای گیاهی تأثیرگذار باشد. | ||
| کلیدواژه‌ها | ||
| بیوفیلم؛ ترشح‌های ریشه؛ عناصر خاک؛ Bacillus subtilis | ||
| عنوان مقاله [English] | ||
| Influence of some environmental and nutritional conditions on biofilm formation of probiotic Bacillus subtilis strains | ||
| نویسندگان [English] | ||
| Maryam Khezri | ||
| Assistant Professor, Department of Plant Protection, Faculty of Agriculture, Urmia University, Urmia, Iran | ||
| چکیده [English] | ||
| Biofilm formation in bacteria is an important survival strategy. Biofilm of probiotic Bacillus subtilis consist of long chains of cells that are covered by a polymeric matrix of exopolysaccharides. This bacterium is known as one of the most effective biocontrol agents against plant pathogens and has a high ability in biofilm formation. This study was aimed to investigate the effect of some environmental conditions including temperature, pH and osmotic pressure; nutritional elements such as sugars and amino acids secreted from wheat root and important soil elements like Ca2+, Mn2+, Mg2+, Fe 2+, Zn2, Mo6+, Co2+, B3+ and Cu2+ on biofilm formation of mentioned bacterium using polystyrene plate. Results of this study indicated that the most amount of biofilm in three studied strains produced at 30°C, pH 7 and the osmotic pressure using concentration of 75% sucrose. Cations of magnesium, calcium, zinc, iron, manganese and copper increased but cations of molybdenum, cobalt and boron decreased biofilm formation. All sugars and amino acids secreted from wheat root increased biofilm formation of studied strains. Among sugars, arabinose and glucose; and among amino acids, lysine and asparagine showed the greatest increasing effect on biofilm formation. According to our results, environmental conditions and the content of nutrient can influence on biofilm formation and consequently, the survival and establishment of probiotic bacteria in their environments, host colonization and biological control against plant pathogens. | ||
| کلیدواژه‌ها [English] | ||
| Bacillus subtilis, biofim, root exudates, soil elements | ||
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