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تأثیر متیل جاسمونات بر فعالیت آنزیم‌های آنتی‌اکسیدان و محتوای عناصر غذایی لوبیا چشم‌بلبلی تحت تنش شوری | ||
| به زراعی کشاورزی | ||
| مقاله 8، دوره 19، شماره 3، آذر 1396، صفحه 653-669 اصل مقاله (650.34 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22059/jci.2017.60470 | ||
| نویسنده | ||
| امید صادقی پور* | ||
| دانشیار، گروه زراعت، واحد یادگار امام خمینی (ره) شهر ری، دانشگاه آزاد اسلامی، تهران، ایران | ||
| چکیده | ||
| به‌منظور بررسی نقش متیل جاسمونات در تحمل به تنش شوری در لوبیاچشم‌بلبلی آزمایشی گلدانی به‌صورت فاکتوریل در قالب طرحی کاملاً تصادفی با سه تکرار در سال 1394 در شهرستان ری انجام شد. تیمارها شامل سه سطح خیساندن بذرها در محلول متیل جاسمونات (صفر، 25 و 50 میکرو مولار) به‌مدت 20 ساعت و سه سطح شوری آب آبیاری (صفر، 50 و 100 میلی‌مولار کلرید سدیم) بود. نتایج نشان داد با افزایش سطح شوری، پراکسایش چربی‌ها، فعالیت آنزیم‌های آنتی‌اکسیدان (سوپراکسید دیسموتاز، کاتالاز، اسکوربات پراکسیداز و گلوتاتیون ردکتاز) و غلظت کلر در برگ افزایش می‌یابد، در حالی که غلظت نیتروژن، کلسیم و منیزیم، همچنین نسبت پتاسیم به سدیم کاهش یافت. این تغییرات در نهایت، به کاهش عملکرد دانه انجامید. از سوی دیگر، تیمار بذر با متیل جاسمونات در شرایط عدم تنش تأثیر معناداری بر صفات اندازه‌گیری‌شده نداشت، ولی کاربرد آن (به‌ویژه غلظت 50 میکرومولار) تحت تنش شوری، موجب فعالیت بیشتر آنزیم‌های آنتی‌اکسیدان، کاهش پراکسایش چربی‌ها، کاهش غلظت کلر در برگ، افزایش غلظت نیتروژن، کلسیم و منیزیم، همچنین نسبت پتاسیم به سدیم شد. در نتیجه موجب بهبود عملکرد شد. براساس نتایج این پژوهش، متیل جاسمونات در القای تحمل به شوری از طریق افزایش فعالیت آنزیم‌های آنتی‌اکسیدان و تغییر در ترکیب عناصر غذایی در لوبیاچشم‌بلبلی نقش مهمی ایفا می‌کند. | ||
| کلیدواژه‌ها | ||
| پراکسایش چربی‌ها؛ پیش‌تیمار بذر؛ تنظیم‌کننده‌های رشد گیاهی؛ عملکرد؛ کلرید سدیم | ||
| عنوان مقاله [English] | ||
| Effect of methyl jasmonate on antioxidant enzymes activity and nutrients content of cowpea under salinity stress | ||
| نویسندگان [English] | ||
| Omid Sadeghipour | ||
| چکیده [English] | ||
| In order to investigate the role of methyl jasmonate on salinity tolerance of cowpea, a factorial pot experiment was conducted in the basis of completely randomized design with three replications in the Rey region in 2015. Treatments included three levels of seed soaking in methyl jasmonate solution (0, 25 and 50 µM) for 20 hours and three levels of salinity (0, 50 and 100 mM NaCl). The results showed that by increasing salinity levels, lipids peroxidation, antioxidant enzymes activity (superoxide dismutase, catalase, ascorbate peroxidase and glutathione reductase) and Cl- concentration in the leaves increased, while the concentration of N, Ca2+ and Mg2+ decreased as well as K+/Na+ ratio. These changes eventually led to decreasing seed yield. Seed treatment with methyl jasmonate had not significantly effect on any measured traits under normal conditions, however this treatment (especially 50 µM) under salinity stress conditions caused further activity of antioxidant enzymes, declining lipids peroxidation, reducing the concentration of Cl- in the leaves, increasing the concentration of N, Ca2+ and Mg2+ as well as K+/Na+ ratio, and finally improved seed yield. These findings suggest that methyl jasmonate plays a pivotal role in inducing salinity tolerance of cowpea plants via enhancing antioxidant enzymes activity and altering nutrients composition. | ||
| کلیدواژه‌ها [English] | ||
| lipids peroxidation, plant growth regulators, seed pre-treatment, Sodium Chloride, yield | ||
| مراجع | ||
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کافی م، برزویی ا، صالحی م، کمندی ع، معصومی ع و نباتی ج (1388) فیزیولوژی تنش‌های محیطی در گیاهان. انتشارات جهاد دانشگاهی مشهد. 502 ص. Abdelgawad ZA (2014) Improving growth and yield of salt-stressed cowpea plants by exogenous application of ascobin. Life Science Journal. 11: 43-51. Abdelgawad ZA, Khalafaallah AA and Abdallah MM (2014) Impact of methyl jasmonate on antioxidant activity and some biochemical aspects of maize plant grown under water stress condition. Agricultural Sciences.5: 1077-1088. Aebi H (1984) Catalase in vitro. Methods in Enzymology. 105: 121-126. Aftab T, Khan MMA, Teixeira da Silva JA, Idrees M and Moinuddin MN (2011) Role of salicylic acid in promoting salt stress tolerance and enhanced artemisinin production in Artemisia annua L. Journal of Plant Growth Regulation. 30(4): 425-435. Arshi A, Abdin MZ and Iqbal M (2005) Ameliorative effect of CaCl2 on growth, ionic relations and proline content of senna under salinity stress. Journal of Plant Nutrition. 28: 101-125. Asik BB, Turanm A, Celik H and Katkat AV (2009) Effects of humic substances on plant growth and mineral nutrients uptake of wheat (Triticum durum cv. Salihi) under conditions of salinity. Asian Journal of Crop Science. 1(2): 87-95. Azooz MM (2009). Salt stress mitigation by seed priming with salicylic acid in two faba bean genotypes differing in salt tolerance. International Journal of Agriculture andBiology. 11: 343-350. Azooz MM, Metwally A and Abou-Elhamd MF (2015) Jasmonate-induced tolerance of Hassawi okra seedlings to salinity in brackish water. Acta Physiologiae Plantarum. 37: 77. Beyer WF and Fridovich I (1987) Assaying for superoxide dismutase activity: some large consequences of minor changes in conditions. Analytical Biochemistry. 161(2): 559-566. Chapman HD and Pratt PF (1961) Methods of analysis for soils, plants and water. University of California, Berkelery, CA, USA. 309 p. Dar TA, Uddin M, Khan MMA, Hakeem KR and Jaleel H (2015) Jasmonates counter plant stress: A Review. Environmental and Experimental Botany. 115: 49-57. Dhaka BR, Pathan ARK and Dhayal RS (2013) Response of cowpea to organic manures and soil salinity. Crop Research. 45: 192-195. El-Beltagi HS, Mohamed HI, Mohammed AHMA, Zaki LM and Mogazy AM (2013) Physiological and biochemical effects of γ-irradiation on cowpea plants (Vigna sinensis) under salt stress. Notulae Botanicae Horti Agrobotanici Cluj-Napoca. 41(1): 104-114. El-Sayed OM, El-Gammal OHM and Salama ASM (2014) Effect of ascorbic acid, proline and jasmonic acid foliar spraying on fruit set and yield of manzanillo olive trees under salt stress. Scientia Horticulturae. 176: 32-37. Foyer CH and Halliwell B (1976) Presence of glutathione and glutathione reductase in chloroplasts: a proposed role on ascorbic acid metabolism. Planta. 133: 21-25. Gupta B and Huang B (2014) Mechanism of salinity tolerance in plants: physiological, biochemical and molecular characterization. International Journal of Genomics, Volume 2014, Article ID 701596, 18 pp. Hadi F, Hussain F and Arif M (2012) Growth performance and comparison of cowpea varieties under different NaCl salinity stresses. Greener Journal of Physical Sciences. 2(1): 44-49. Hassan MY, Gregorio W and John B (1983) Activity and conformational changes in chloroplast coupling factor induced by ion binding: formation of magnesium–enzyme–phosphate-complex. Biochemistry. 22: 2502-2512. Hasanuzzaman M, Nahar K and Fujita M (2013) Plant response to salt stress and role of exogenous protectants to mitigate salt-induced damages. In: Ahmad P, Azooz MM and Prasad MNV (Eds.). Ecophysiology and responses of plants under salt stress. Springer, New York, USA, pp 25-87. Heath RL and Packer L (1968) Photoperoxidation in isolated chloroplasts. I. Kinetics and stoichiometry of fatty acid peroxidation. Archives of Biochemistry and Biophysics. 125(1): 189-198. Hozayn M, Abd El-Monem AA, Ebtihal MAEM and Amira MSA (2013) Amelioration of salinity stress in mungbean (Vigna radiate L) plant by soaking in arginine. Journal of Applied Sciences Research. 9(1): 393-401. Jain V, Vart S, Verma E and Malhotra SP (2015) Spermine reduces salinity-induced oxidative damage by enhancing antioxidative system and decreasing lipid peroxidation in rice seedlings. Journal of Plant Biochemistry and Biotechnology. 24(3): 316-323. Kamiab F, Talaie A, Khezri M and Javanshah A (2014) Exogenous application of free polyamines enhance salt tolerance of pistachio (Pistacia vera L.) seedlings. Journal of Plant Growth Regulation. 72: 257-268. Kang DJ, Seo YJ, Lee JD, Ishii R, Kim KU, Shin DH, Park SK, Jang SW and Lee IJ (2005). Jasmonic acid differentially affects growth, ion uptake and abscisic acid concentration in salt-tolerant and salt-sensitive rice cultivars. Journal of Agronomy and Crop Science. 191: 273-282. Khan NA, Syeed S, Masood A, Nazar R and Iqbal N (2010) Application of salicylic acid increases contents of nutrients and antioxidative metabolism in mung bean and alleviates adverse effects of salinity stress. International Journal of Plant Biology. 1: 1-8. Kumari A, Sheokand S and Swaraj K (2010) Nitric oxide induced alleviation of toxic effects of short term and long term Cd stress on growth, oxidative metabolism and Cd accumulation in chickpea. Brazilian Journal of Plant Physiology. 22: 271-284. Li H, Zhu Y, Hu Y, Han W and Gong H (2015) Beneficial effects of silicon in alleviating salinity stress of tomato seedlings grown under sand culture. Acta Physiologiae Plantarum. 37: 71. Miller G, Suzuki N, Ciftci-Yilmazi N and Mittler R (2010) Reactive oxygen species homeostasis and signalling during drought and salinity stresses. Plant, Cell and Environment. 33: 453-467. Munns R and Tester M (2008) Mechanisms of salinity tolerance. Annual Review of Plant Biology. 59: 651-681. Murillo-Amador B, Troyo-Die´guez E, Garcı´a-Herna´ndez JL, Lopez-Aguilar R, Avila-Serrano NY, Zamora-Salgado S, Rueda-Puente EO and Kaya C (2006). Effect of NaCl salinity in the genotypic variation of cowpea (Vigna unguiculata) during early vegetative growth. Scientia Horticulturae. 108: 423-431. Nakano Y and Asada K (1981) Hydrogen peroxide is scavenged by ascorbate specific peroxidase in spinach chloroplasts. Plant and Cell Physiology. 22(5): 867-880. Parida AK and Das AB (2005) Salt tolerance and salinity effects on plants: a review. Ecotoxicology and Environmental Safety. 60: 324-349. Patel PR, Kajal SS, Patel VR, Patel VJ and Khristi SM (2010) Impact of salt stress on nutrient uptake and growth of cowpea. Brazilian Journal of Plant Physiology. 22(1): 43-48. Pedranzani H, Racagni G, Alemano S, Miersch O and Pena-cortes H (2003) Salt tolerant tomato plants show increased levels of jasmonic acid. Journal of Plant Growth Regulation. 14: 149-158. Sadak MS and Abdelhamid MT (2015). Influence of amino acids mixture application on some biochemical aspects, antioxidant enzymes and endogenous polyamines of Vicia faba plant grown under seawater salinity stress. Gesunde Pflanzen. 67: 119-129. Seif SN, Tafazzoli E, Talaii AR, Aboutalebi A and Abdosi V (2014) Evaluation of two grape cultivars (Vitis vinifera L.) against salinity stress and surveying the effect of methyl jasmonate and epibrassinolide on alleviation the salinity stress. International Journal ofBiosciences.5(7): 116-125. Sheteawi SA (2007) Improving growth and yield of salt-stressed soybean by exogenous application of jasmonic acid and ascobin. International Journal of Agriculture and Biology. 9(3): 473-478. Singh I and Shah K (2014) Exogenous application of methyl jasmonate lowers the effect of cadmium-induced oxidative injury in rice seedlings. Phytochemistry. 108: 57-66. Sultana N, Ikeda T and Kashem MA (2001) Effect of foliar spray of nutrient solutions on photosynthesis, dry matter accumulation and yield in seawater–stressed rice. Environmental and Experimental Botany. 46: 129-140. Syeed S, Anjum NA, Nazar R, Iqbal N, Masood A and Khan NA (2011) Salicylic acid-mediated changes in photosynthesis, nutrients content and antioxidant metabolism in two mustard (Brassica juncea L.) cultivars differing in salt tolerance. Acta Physiologiae Plantarum. 33: 877-886. Tartoura KAH, Youssef SA and Tartoura EAA (2014) Compost alleviates the negative effects of salinity via upregulation of antioxidants in Solanum lycopersicum L. plants. Journal of Plant Growth Regulation. 74: 299-310. Walia H, Wilson C, Condamine P, Liu X, Ismail AM and Close TJ (2007) Large-scale expression profiling and physiological characterization of jasmonic acid-mediated adaptation of barley to salinity stress. Plant, Cell and Environment. 30: 410-421. Yoon JY, Hamayun M, Lee SK and Lee IJ (2009) Methyl jasmonate alleviated salinity stress in soybean. Journal of Crop Science and Biotechnology. 12(2): 63-68.
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