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Identification and Expression of Genes Involved in the Biosynthesis of Penicillin and Its Detection by HPLC in Penicillium chrysogenum | ||
| Journal of Sciences, Islamic Republic of Iran | ||
| مقاله 1، دوره 28، شماره 2، تیر 2017، صفحه 105-111 اصل مقاله (1.11 M) | ||
| نوع مقاله: Final File | ||
| نویسندگان | ||
| SF. Miri* 1؛ M.A. Tajick2؛ H. Rahimian1؛ G. Nematzadeh2 | ||
| 11Department of Plant protection, Faculty of Sciences, University of Sari Agricultural Sciences and Natural Resources, Sari, Islamic Republic of Iran | ||
| 22Genetic and Agricultural Biotechnology Institute of Tabarestan (GABIT), University of Sari Agricultural Sciences and Natural Resources, Sari, Islamic Republic of Iran | ||
| چکیده | ||
| In this study, after identification of genes involved in biosynthesis of penicillin, we evaluated the expression of pcbAB and pcbC genes in P. Chrysogenum. A quantitative PCR (qPCR) approach was used to determine how these genes were expressed in different time courses. In addition, the produced penicillin content was measured using HPLC. qPCR analysis of mRNAs extracted from P. chrysogenum indicated that the expression levels of pcbAB, pcbC increased in seven days after inoculation compared to the expressed levels with others time courses, but this difference was not significant statistically. The analysis of chromatograms from injected showed that the highest content of penicillin in media observed in 7 days after cultivation, except in P. chrysogenum PTCC 5033. In addition, the results of comparative analysis among the tested strains showed that the penicillin content in 5, 7 and 11 days after cultivation was highest in P. chrysogenum PTCC 5037, P. chrysogenum PTCC 5031 and P. chrysogenum PTCC 5033, respectively. However, the results showed an evident relationship between the expression levels of penicillin biosynthesis genes and yielded penicillin. | ||
| کلیدواژهها | ||
| secondary metabolites؛ Penicillin؛ gene expression؛ HPLC | ||
| مراجع | ||
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