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تأثیر بیوفیلم تولید شده توسط ریزوباکتریهای پروبیوتیک گیاهی بر کلنیزاسیون ریشه و رشد گیاه گندم | ||
کنترل بیولوژیک آفات و بیماری های گیاهی | ||
مقاله 9، دوره 6، شماره 1، خرداد 1396، صفحه 93-102 اصل مقاله (514.22 K) | ||
نوع مقاله: مقاله پژوهشی | ||
شناسه دیجیتال (DOI): 10.22059/jbioc.2017.220985.178 | ||
نویسنده | ||
مریم خضری* | ||
استادیار گروه گیاه پزشکی، دانشکده کشاورزی، دانشگاه ارومیه | ||
چکیده | ||
به منظور بررسی پتانسیل باکتری پروبیوتیک Bacillus subtilis در تولید بیوفیلم و کلنیزاسیون ریشه گندم، از 11 سویه این باکتری استفاده شد. آزمایشها در قالب طرح کاملا تصادفی و در چهار تکرار انجام شد. بنابر نتایج حاصل از این پژوهش، میزان تولید بیوفیلم و بیوسورفکتانتها در ریزوباکتریهای مورد مطالعه تنوع بالایی نشان داد و بیشترین مقدار تولید این متابولیتها در سویههای B3، B1 و B4 دیده شد. بررسی میزان کلنیزاسیون با استفاده از باکتریهای موتانت مقاوم به آنتیبیوتیکهای ریفامپیسین و نالیدیکسیکاسید انجام شد. سویههای B4 و B3 به ترتیب با جمعیتهای FU/g 107×41/4 و cfu/g 107×35/4، بیشترین میزان کلنیزاسیون ریشه را نسبت به سایر سویهها داشتند. میزان تشکیل بیوفیلم و تولید بیوسورفکتانتها با کلنیزاسیون ریشه گندم به ترتیب 74/0 و 80/0 همبستگی داشتند. در بخش دیگر این تحقیق، اثر این سویهها روی فاکتورهای رشدی گیاه گندم مورد ارزیابی قرار گرفت. کلیه سویهها موجب افزایش طول ریشه و اندام هوایی شدند و بیشتر سویهها اثر افزایشی در وزن خشک ریشه و اندام هوایی نشان دادند. براساس یافتههای این پژوهش، سویههای باکتری که پتانسیل بالایی در تولید بیوفیلم و بیوسورفکتانتها داشتند، ریشه گندم را به خوبی کلنیزه نمودند و غالب سویهها اثر افزاینده روی فاکتورهای رشدی گندم نشان دادند. | ||
کلیدواژهها | ||
باسیلوس سابتیلیس؛ بیوفیلم؛ بیوسورفکتانتها؛ پروبیوتیک؛ محرک رشد | ||
عنوان مقاله [English] | ||
Effect of biofilm by plant probiotic rhizobacteria on root colonization and growth of wheat | ||
نویسندگان [English] | ||
Maryam Khezri | ||
Assistant Professor, Department of Plant Protection, Faculty of Agriculture, Urmia University, Urmia, Iran | ||
چکیده [English] | ||
A total of 11 probiotic strains of Bacillus subtilis were used to evaluate the potential of biofilm formation and wheat root colonization. Experiments carried out in a completely randomized design with four replications. According to results of this study, biofilm formation and biosurfactants production showed a high diversity in studied rhizobacteria, B3, B1 and B4 strains produced the maximum amount of mentioned metabolites. Evaluation of root colonization was performed using rifampicin and nalidixic acid resistant mutants. B4 and B3 with 4.41×107 cfu/gand 4.35×107 cfu/g had the highest root colonization compared to other strains. Correlation of 0.74 and 0.80 was obtained between biofilm formation and biosurfactants production with wheat root colonization, respectively. In another part of this study, the effects of studied strains were evaluated on wheat plant growth factors. All probiotic rhizobacteria strains increased the length of roots and aerial part of wheat and most of them increased dry weight of roots and aerial part. Based on the findings of this research, strains with high potential in biofilm formation and biosurfactants production were colonized wheat root very well and most of studied strains showed the effect of increasing on wheat growth factors. | ||
کلیدواژهها [English] | ||
Bacillus subtilis, biofilm, biosurfactants, growth promoting, Probiotic | ||
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