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جداسازی و شناسایی سیانوباکترها از خاکهای فوق شور پارک ملی کویر | ||
تحقیقات آب و خاک ایران | ||
مقاله 15، دوره 48، شماره 3، مهر 1396، صفحه 625-637 اصل مقاله (698.34 K) | ||
نوع مقاله: مقاله پژوهشی | ||
شناسه دیجیتال (DOI): 10.22059/ijswr.2017.212139.667506 | ||
نویسندگان | ||
عاطفه اعتمادی خواه* 1؛ احمد علی پوربابایی1؛ حسینعلی علیخانی1؛ مصطفی نوروزی2 | ||
1دانشگاه تهران | ||
2دانشگاه الزهرا | ||
چکیده | ||
سیانوباکترها یک گروه منحصر به فرد از باکتریهای فتواتوتروف هستند که برخی از آنها به دلیل ویژگیهای ساختاری، تحمل قابل توجهی به تنش شوری نشان میدهند. این موجودات نقش مهمی در محیطهای خاکی به ویژه در نواحی خشک و نیمهخشک ایفا میکنند. در مطالعه حاضر سیانوباکترهای خاک از مناطق بیابانی ایران جداسازی شده و سپس جدایههای مقاوم به شرایط فوق شور شناسایی شدند. 40 نمونه خاک از پارک ملی کویر تهیه شد. نمونهها پس از کشت در محیط BG11 و ASN III (5/3، 5، 6 و 7 درصد کلرید سدیم) و انکوباسیون در شرایط مناسب دمایی و نور، جداسازی شده و با استفاده از کلیدهای مورفولوژیکی به طور اولیه و سپس با روش مولکولی مورد شناسایی قرار گرفتند. از مجموع 40 مکان نمونهبرداری فقط از 4 مکان سویههای سیانوباکتر مقاوم به شرایط فوق شور جداسازی شد. در محیط کشت فاقد کلرید سدیم 27 مورفوتایپ شناسایی شد که پنج سویه متعلق به دو جنس قادر به رشد در حضور 5/3 درصد کلرید سدیم بودند. در میان این پنج سویه، سه سویه در غلظت 5 و دو سویه در غلظت 6 درصد کلرید سدیم رشد کردند. تنها سویه Phormidium autumnale 61et قادر به تحمل غلظت 7 درصد کلرید سدیم بود که از مکانی با شوری 80/87 دسی زیمنس بر متر جداسازی شده بود. نتایج نشان داد که برخی از سیانوباکترهای جدا شده از خاک پارک ملی کویر قادرند در شرایط سخت مانند تنش شوری رشد کنند و توده بیولوژیکی فعالی را ایجاد نمایند. | ||
کلیدواژهها | ||
بیابان؛ خاک؛ سیانوباکتر؛ 16S rRNA | ||
عنوان مقاله [English] | ||
Isolation and identification of cyanobacteria from Kavir National Park hypersaline soils | ||
نویسندگان [English] | ||
Atefeh Etemadi-Khah1؛ AhmadAli Poorbabaei1؛ Hoseinali Alikhani1؛ Mostafa Noroozi2 | ||
1University of Tehran | ||
2Ahzahra University | ||
چکیده [English] | ||
Cyanobacteria are a unique group of photoautotrophic bacteria that some of them due to their structural characteristics show a significant tolerance to salinity. These organisms play an important role in terrestrial environments, especially in arid and semi-arid region. In this study, soil cyanobacteria were isolated from the desert regions of Iran and then isolates resistant to hypersaline conditions identified. 40 soil samples were collected from the Kavir National Park. Samples were separated after culture in BG11 and ASN III (3.5, 5, 6 and 7% of NaCl) mediums and incubated under appropriate conditions of temperature and light and identified initially using morphological keys and then by molecular methods. Out of 40 sampling sites cyanobacteria were isolated only from four hypersaline sites. Twenty-seven morphotypes were identified in medium without sodium chloride that five strains belonging to two genera were able to grow in the presence of 3.5% sodium chloride. Among the five strains, three strains in 5% and two strains in 6% of sodium chloride grew. Only Phormidium autumnale 61et was able to tolerate 7% sodium chloride that was isolated from the site with 87.80 dS/m. The results showed that some isolated cyanobacteria from soils of Kavir National Park can grow in extreme conditions such as salinity stress and create a biologically active mass. | ||
کلیدواژهها [English] | ||
16S rRNA, Cyanobacteria, Desert, soil | ||
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