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بهینه سازی تولید بیوپلیمر پولولان توسط Aureobasidium pullulans از ضایعات انار | ||
| مهندسی بیوسیستم ایران | ||
| مقاله 9، دوره 48، شماره 3، مهر 1396، صفحه 319-327 اصل مقاله (671.25 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22059/ijbse.2017.232470.664935 | ||
| نویسندگان | ||
| حمیرا میرزایی1؛ فرامرز خدائیان* 2؛ محمد جوان نیکخواه3 | ||
| 1دانشجو کارشناسی ارشد علوم و مهندسی صنایع غذایی | ||
| 2دانشیار، گروه علوم و مهندسی صنایع غذیی ، پردیس کشاورزی و منابع طبی,ی ، دانشگاه تهران | ||
| 3استاد گروه گیاه پزشکی، پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج | ||
| چکیده | ||
| در این مطالعه بهینه سازی شرایط تخمیر جهت تولید پولولان از ضایعات انار توسط Aureobasidium pullulans (با شماره دستیابی KY767023 ) مورد بررسی قرار گرفت. روش بهینه‌یابی سطح پاسخ [a1] به منظور بررسی اثرات سه عامل pH اولیه، درصد تلقیح و نسبت رطوبت اولیه به سوبسترا بر میزان تولید پولولان در محیط سوبسترای جامد (SSF) مورد استفاده قرار گرفت. نتایج این آزمایشات نشان داد که حداکثر تولید پولولان در شرایط بهینه (pH اولیه: 75/7، درصد تلقیح: %5 حجمی/حجمی و نسبت رطوبت اولیه به سوبسترا: 5/2) g/kg 35/0 ± 5/50 است. بررسی ویژگی‌های فیزیکوشیمیایی پولولان به دست آمده در شرایط بهینه نیز مورد مطالعه قرار گرفت. فعالیت امولسیفایری پولولان استخراجی 63 درصد و پایداری امولسیون محلول پولولان به ترتیب در دمای °C 4 و °C 25 پس از 30 روز نگهداری، 5/60 و 5/58 درصد گزارش شد. نتایج حاصل از اندازه گیری ویسکوزیته در غلظت های مختلف نشان داد که محلول پولولان در غلظت‌های پایین (5/0-1/0 درصد حجمی/ وزنی) دارای رفتار جریان نزدیک به نیوتنی بوده و با افزایش غلظت محلول، از نیوتنی به رقیق شونده با برش تغییر رفتار می‌دهد. بررسی طیف سنجی FT-IR پولولان بدست آمده از ضایعات انار حضور پیوندهای (6→1) α را در واحدهای مالتوتریوز تایید می‌کند. | ||
| کلیدواژه‌ها | ||
| پولولان؛ ضایعات انار؛ روش سطح پاسخ؛ درصد تلقیح؛ FT-IR | ||
| عنوان مقاله [English] | ||
| Optimization of pullulan production from pomegranate waste by A.pullulans | ||
| نویسندگان [English] | ||
| homaira mirzaee1؛ Faramarz Khodaiyan2؛ Mohammad Javan Nikkhah3 | ||
| 1Former Graduate, Food Science & Engineering, College of Agriculture and Natural Resources, University of Tehran, Karaj | ||
| 2Associate Professor, Food Science & Engineering, College of Agriculture and Natural Resources, University of Tehran, Karaj | ||
| 3Professor,Department of Plant protection, College of Agriculture and Natural Resources, University of Tehran, Karaj | ||
| چکیده [English] | ||
| In this study, optimization of fermentation conditions for pullulan production by Aureobasidium pullulans (KY767023) from pomegranate waste was investigated. Response surface methodology was used to evaluate the effects of three factors (initial pH, inoculum size and ratio of liquid to solid) on pullulan yield in solid-state fermentation (SSF). Results of these experiments indicated that maximum pullulan concentration (50.5 ± 0.35 g/kg) was obtained at the optimum levels of process variables (initial pH 7.75, inoculum size 5% v/v, ratio of liquid to solid 1:2.5). Furthermore, physicochemical characteristics of the obtained pullulan at the optimum conditions were studied. The emulsifying activity of extracted pullulan was 63% .The emulsion stability was 60.5% at 4 °C and 58.5% at 25 °C after 30 days of storage. The results of viscosity curves at different concentration demonstrated that the solutions of pullulan at low concentrations (0.1-0.5 % w/v) showed nearly Newtonian flow behavior, and as the concentration of pullulan solution increased, the behavior changes from Newtonian to pseudoplastic flow. FT-IR of the pullulan produced from pomegranate by-products revealed the presence of α (1→6) linked maltotriose units. | ||
| کلیدواژه‌ها [English] | ||
| pullulan, pomegranate waste, Response Surface Methodology, inoculum size, FT-IR | ||
| مراجع | ||
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