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محمدی, حسین, رافت, سید عباس, مرادی شهربابک, حسین, شجاع, جلیل, مرادی, محمد حسین. (1396). پویش ژنگان کل برای تعیین جایگاه‌های تحت انتخاب مثبت در گوسفندان نژاد زندی. , 48(4), 533-548. doi: 10.22059/ijas.2017.229950.653520
حسین محمدی; سید عباس رافت; حسین مرادی شهربابک; جلیل شجاع; محمد حسین مرادی. "پویش ژنگان کل برای تعیین جایگاه‌های تحت انتخاب مثبت در گوسفندان نژاد زندی". , 48, 4, 1396, 533-548. doi: 10.22059/ijas.2017.229950.653520
محمدی, حسین, رافت, سید عباس, مرادی شهربابک, حسین, شجاع, جلیل, مرادی, محمد حسین. (1396). 'پویش ژنگان کل برای تعیین جایگاه‌های تحت انتخاب مثبت در گوسفندان نژاد زندی', , 48(4), pp. 533-548. doi: 10.22059/ijas.2017.229950.653520
محمدی, حسین, رافت, سید عباس, مرادی شهربابک, حسین, شجاع, جلیل, مرادی, محمد حسین. پویش ژنگان کل برای تعیین جایگاه‌های تحت انتخاب مثبت در گوسفندان نژاد زندی. , 1396; 48(4): 533-548. doi: 10.22059/ijas.2017.229950.653520

پویش ژنگان کل برای تعیین جایگاه‌های تحت انتخاب مثبت در گوسفندان نژاد زندی

علوم دامی ایران
مقاله 6، دوره 48، شماره 4، اسفند 1396، صفحه 533-548    اصل مقاله (1008.77 K)
نوع مقاله: مقاله پژوهشی
شناسه دیجیتال (DOI): 10.22059/ijas.2017.229950.653520
نویسندگان
حسین محمدی1؛ سید عباس رافت2؛ حسین مرادی شهربابک* 3؛ جلیل شجاع4؛ محمد حسین مرادی5
1دانشجوی دکتری گروه علوم دامی، دانشکدۀ کشاورزی، دانشگاه تبریز
2استاد گروه علوم دامی، دانشکدۀ کشاورزی، دانشگاه تبریز
3استادیار گروه علوم دامی، پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج
4گروه علوم دامی، دانشکده کشاورزی، دانشگاه تبریز.
5استادیار گروه علوم دامی، دانشکدۀ کشاورزی و منابع طبیعی، دانشگاه اراک
چکیده
شناسایی مناطق ژنگانی (ژنوم) که هدف انتخاب بوده­اند، یکی از راهکار‌های اصلی تحقیقات زیستی است. هدف این پژوهش، پویش کل ژنگان برای شناسایی مناطقی از ژنگان گوسفندان نژاد زندی که در طی سال­های مختلف هدف انتخاب­های طبیعی یا مصنوعی قرار گرفته‌اند، بود. بدین منظور 96 رأس گوسفند نژاد زندی با استفاده از آرایه­های ژنگانی Illumina ovine SNP50K BeadChip تعیین ژنوتیپ شدند. برای شناسایی نشانه­های انتخاب بر پایۀ روش­های نداشتن تعادل پیوستگی (لینکاژی) از آزمون (iHS)integrated haplotype score استفاده شد. نتایج یازده منطقۀ ژنگانی روی کروموزوم­های 1 (2 منطقه)، 3، 6، 7، 8، 9، 10، 17، 22 و 26 را شناسایی کرد. تجزیه‌وتحلیل داده‌های زیستی (بیوانفورماتیکی) نشان داد، برخی از این مناطق ژنگانی به‌طور مستقیم و غیرمستقیم با ژن­های مؤثر بر سازگاری (آداپتاسیون) به آب‌وهوای گرم و خشک (DNAJB4، ­HSPA4L، MSRB3­)، پاسخ ایمنی (IL23A، STAT6، LY96)، توسعه و اندازۀ بدن (STAC3، LAP3)، توسعۀ نظام ساختار بدنی یا اسکلتی (SPP1، MEPE، IBSP) و سوخت‌وساز (متابولیسم) انرژی (ATP5B، GLS2، CS) همپوشانی دارند. درنهایت بررسیQTLهای گزارش‌شده در ژنگان گوسفند نشان داد، این مناطق با QTLهای صفات مهم اقتصادی از جمله صفات مرتبط با رشد، لاشه و پشم در ارتباط است. به‌هرحال، برای شناسایی دقیق این ژن­ها و QTLها لازم است بررسی‌های پیوستگی و عملکردی بیشتری انجام شود.
کلیدواژه‌ها
آزمونiHS؛ پویش ژنگانی؛ سازگاری؛ شناسایی نشانه‌های انتخاب
عنوان مقاله [English]
Genome-wide analysis for detection of loci under positive selection in Zandi sheep breed
نویسندگان [English]
Hossein Mohammadi1؛ Seyed Abbas Rafat2؛ Hossein Moradi Shahrbabak3؛ Jalil Shoja4؛ mohammad hosein moradi5
1Ph.D. Candidate, Department of Animal Science, Faculty of Agricultural Sciences, University of Tabriz, Iran
2Professor, Department of Animal Science, Faculty of Agricultural Sciences, University of Tabriz, Iran
3Assistant Professor, Department of Animal Sciences, University College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
4Department of Animal Science, Faculty of Agricultural Sciences, University of Tabriz.
5Assistant Professor, Department of Animal Sciences, Faculty of Agriculture and Natural Resources, University of Arak, Iran
چکیده [English]
Identification of selection targeted genomic regions is one of the main aims of biological research.The objective of this study was a genome-wide scan to identify the genomic regions that have been under artificial and natural selection in Zandi sheep breed. For this purpose, 96 animal of Zandi breed have been genotyped using the Illumina ovine SNP50 BeadChip. The intergrated hapl-type score (iHS) test was used to detect the selection sweep, due to linkage disequilibrium, associated with these signatures. The results revealed eleven genomic regions on 1 (two areas), 3, 6, 7,8, 9, 10, 17, 22 and 26 chromosomes. Bioinformatics analysis demonstrated that some of these genomic regions overlapped with reported genes that directly and indirectly influenced traits for adaptation to hot arid environments (DNAJB4, HSPA4L, MSRB3), immune response (IL23A, STAT6, LY96), body size and development (STAC3, LAP3), development of the skeletal system (SPP1, MEPE, IBSP) and energy and digestive metabolism (ATP5B, GLS2, CS). Finally, study of the reported QTL in these regions of the sheep genome showed that they overlapped with QTL of economically important traits such as carcass yield, growth and wool traits. However, it will be necessary to carry out more association and functional studies to demonstrate the implication of these genes.
کلیدواژه‌ها [English]
Adaptation, genome scan, iHS test, selection signature detection
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آمار
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