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Ebrahimi, L., Fotouhifar, K., Javan Nik Khah, M., Naghavi, M. (2018). Diversity and population genetic structure of Venturia inaequalis on different apple cultivars in the north of Iran. , 49(1), 23-31. doi: 10.22059/ijpps.2017.215320.1006734
Leila Ebrahimi; Khalil-Berdi Fotouhifar; Mohammad Javan Nik Khah; Mohammad-Reza Naghavi. "Diversity and population genetic structure of Venturia inaequalis on different apple cultivars in the north of Iran". , 49, 1, 2018, 23-31. doi: 10.22059/ijpps.2017.215320.1006734
Ebrahimi, L., Fotouhifar, K., Javan Nik Khah, M., Naghavi, M. (2018). 'Diversity and population genetic structure of Venturia inaequalis on different apple cultivars in the north of Iran', , 49(1), pp. 23-31. doi: 10.22059/ijpps.2017.215320.1006734
Ebrahimi, L., Fotouhifar, K., Javan Nik Khah, M., Naghavi, M. Diversity and population genetic structure of Venturia inaequalis on different apple cultivars in the north of Iran. , 2018; 49(1): 23-31. doi: 10.22059/ijpps.2017.215320.1006734

Diversity and population genetic structure of Venturia inaequalis on different apple cultivars in the north of Iran

دانش گیاهپزشکی ایران
Article 3, Volume 49, Issue 1, September 2018, Pages 23-31    PDF (379.97 K)
Document Type: Research Paper
DOI: 10.22059/ijpps.2017.215320.1006734
Authors
Leila Ebrahimi1; Khalil-Berdi Fotouhifar* 2; Mohammad Javan Nik Khah3; Mohammad-Reza Naghavi4
1Assistant Professor, Department of Entomology and Plant Pathology, College of Aburaihan, University of Tehran, Tehran, 33916-53755, Iran and Ph. D. Candidate, Department of Plant Protection, Faculty of Agricultural Science and Engineering, College of Agriculture & Natural Resources, University of Tehran, Karaj, 31587-77871, Iran
2Associate Professor, Department of Plant Protection, Faculty of Agricultural Science and Engineering, College of Agriculture & Natural Resources, University of Tehran, Karaj, 31587-77871, Iran
3Professor, Department of Plant Protection, Faculty of Agricultural Science and Engineering, College of Agriculture & Natural Resources, University of Tehran, Karaj, 31587-77871, Iran
4Professor, Department of Biotechnology, Faculty of Agricultural Science and Engineering, College of Agriculture & Natural Resources, University of Tehran, Karaj, Iran
Abstract
Apple scab disease caused by Venturia inaequalis is the most important economic disease worldwide where the apple is grown and causes annual epidemics. It reduces the quality and quantity of the yields in different regions of Iran. In this study, diversity and population genetic structure of V. inaequalis were surveyed on different apple cultivars using 18 microsatellite markers. 51 isolates were obtained from infected leaf and fruit specimens from wild apple, Iranian endemic and commercial apples from Northern provinces of Iran (Mazandaran, Golestan and Guilan). 28 SSR primers were used to investigate the genetic diversity of this pathogen. Among them, 18 primers showed polymorphism between isolates and populations. AMOVA analyses revealed that 97% of the variation was distributed among individuals within populations, and 3% was attributable to the differences among populations. Gene diversity indexes including Nei’s gene diversity, Shannon index and allele numbers in every population showed that diversity within the population on endemic cultivars is more than the diversity in the population of wild and commercial cultivars. Also, the population of commercial cultivars has more diversity in comparison with the wild population. High genetic diversity within the populations is caused by annual sexual reproduction, gene flow between populations and probably the existence of this fungus for a long time in this region.
Keywords
Apple scab; Genetic diversity; north of Iran; SSR
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