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ارزیابی اثر بیوپرایمینگ بذر بر کنترل زیستی بیماری لکه برگی گوجه فرنگی و فاکتورهای رشدی گیاه | ||
کنترل بیولوژیک آفات و بیماری های گیاهی | ||
مقاله 2، دوره 7، شماره 2، آذر 1397، صفحه 1-16 اصل مقاله (820.3 K) | ||
نوع مقاله: مقاله پژوهشی | ||
شناسه دیجیتال (DOI): 10.22059/jbioc.2018.263489.237 | ||
نویسندگان | ||
مریم خضری* 1؛ افسانه عباس پور انبی2؛ فرزانه محمدسور2 | ||
1هیات علمی گروه گیاه پزشکی دانشگاه ارومیه | ||
2دانشجوی کارشناسی ارشد گروه گیاهپزشکی، دانشکده کشاورزی، دانشگاه ارومیه | ||
چکیده | ||
بیماری لکهبرگی باکتریایی گوجهفرنگی ناشی از باکتری Pseudomonas syringae pv. syringae یکی از عوامل مشکلساز مهم برای تولید این محصول در گلخانه و مزرعه است. در این مطالعه، تاثیر سویههایی از ریزوباکتریهای پروبیوتیک بر شاخصهای بذر، فاکتورهای رشدی گیاه و کنترل بیولوژیک عامل بیماری لکه برگی سیرینگایی گوجهفرنگی در آزمایشگاه و گلخانه مورد ارزیابی قرار گرفت. باکتریهای بیماریزا و پروبیوتیک از مزارع گوجهفرنگی استان آذربایجانغربی جداسازی شدند. شناسایی ریزوباکتریهای مفید با استفاده از روشهای فنوتیپی و مولکولی انجام شد. توانایی 21 سویه ریزوباکتری منتخب از گونههای Bacillus subtilis و Pseudomonas fluorescens در تولید متابولیتهای ثانویه با استفاده از روشهای نقطهگذاری سوسپانسیون فیلتر شده و قرار دادن باکتری پروبیوتیک در چاهک ارزیابی شد. همچنین سویهها از نظر تولید آنزیمهای لیپاز، پروتئاز و پروتئاز قلیایی مورد بررسی قرار گرفتند. سویههای ریزوباکتری در هر دو روش موجب کاهش رشد باکتری بیماریزا در آزمایشگاه شدند و آنزیمهای هیدرولیتیک توسط کلیه سویهها تولید شدند. برخی از سویههای باکتری موجب افزایش درصد و سرعت جوانهزنی بذرهای گوجهفرنگی شدند. در گلخانه، سویههای منتخب باعث افزایش فاکتورهای رشدی گیاه گوجهفرنگی شدند. بیشترین تاثیر در مورد سویههای Pf23 و Bs11 دیده شد. باکتریهای پروبیوتیک موجب کاهش بیماری بین 25 و 25/81 درصد نسبت به شاهد شدند. در گلخانه سویه Bs11 با 25/81% و سویههایBs6 و Pf23 با 50/61% کاهش بیماری نسبت به شاهد، بیشترین تاثیر را در کاهش لکههای ایجاد شده روی برگ نشان دادند. سویههای Bs11 و Pf23 به عنوان سویههای برتر این مطالعه در تاثیر روی فاکتورهای رشدی گیاه و کاهش بیماری لکهبرگی سیرینگایی گوجهفرنگی انتخاب شدند. | ||
کلیدواژهها | ||
Pseudomonas syringae pv. syringae؛ کنترل بیولوژیک؛ سودوموناس فلورسنت؛ باسیلوس سابتیلیس | ||
عنوان مقاله [English] | ||
Evaluation of seed biopriming on tomato leaf spot biocontrol and plant growth factors | ||
نویسندگان [English] | ||
Maryam Khezri1؛ Afsaneh Abbaspour Anbi2؛ Farzaneh Mohammad Sour2 | ||
1Assistant professor, Department of Plant Protection, Faculty of Agriculture, Urmia University, Urmia, Iran. | ||
2Msc. student, Department of Plant Protection, Faculty of Agriculture, Urmia University, Urmia, Iran. | ||
چکیده [English] | ||
Tomato bacterial leaf spot caused by Pseudomonas syringae pv. syringae is a serious problem for tomato production in the greenhouse and field. In this study, effect of some probiotic rhizobacteria evaluated on seed indexes, plant growth factors and biocontrol of syringae leaf spot in the laboratory and greenhouse. All probiotic and pathogenic bacteria isolated from tomato fields in West Azarbaijan. Identification of benefit rhizobacteria carried out via phenotypic and molecular methods. Ability of 21 selected rhizobacteria of Bacillus subtilis and Pseudomonas fluorescens strains in secondary metabolites production was investigated via two methods. In first method, filtered suspension spotted on medium, and in second one bacterial cell was poured in wells created on medium. In addition, the strains were studied for lipase, protease and alkaline protease production. In both methods, studied strains reduced the pathogenic bacterial growth in the laboratory, and hydrolytic enzymes produced by all strains. Some of the studied strains increased percentage and speed of tomato seed germination. Selected strains increased the growth factors of tomato plants in greenhouse. The greatest effect was observed in Pf23 and Bs11 strains. The probiotic bacteria reduced the disease between 25 and 81.25% compared to the control. In the greenhouse, Bs11 with 81.25% and Bs6 and Pf23 with 61.50% disease decreasing compared to control, showed the most effect in reducing leaf spots. Bs11 and Pf23 were selected as the best strains in effect on tomato growth factors and biocontrol of syringae leaf spot. | ||
کلیدواژهها [English] | ||
Pseudomonas syringae pv. syringae. Biological control, Pseudomonas fluorescens, Bacillus subtilis | ||
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