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سمایی, سیده سمیه, پیری, مقصود, دلفان, مریم. (1397). تأثیر یک دوره تمرین تناوبی شدید و استقامتی تداومی بر بیان ژن‌های آدیپونکتین و TNF-α در رت‌های مبتلا به دیابت نوع دو. , 10(3), 317-332. doi: 10.22059/jsb.2018.247817.1227
سیده سمیه سمایی; مقصود پیری; مریم دلفان. "تأثیر یک دوره تمرین تناوبی شدید و استقامتی تداومی بر بیان ژن‌های آدیپونکتین و TNF-α در رت‌های مبتلا به دیابت نوع دو". , 10, 3, 1397, 317-332. doi: 10.22059/jsb.2018.247817.1227
سمایی, سیده سمیه, پیری, مقصود, دلفان, مریم. (1397). 'تأثیر یک دوره تمرین تناوبی شدید و استقامتی تداومی بر بیان ژن‌های آدیپونکتین و TNF-α در رت‌های مبتلا به دیابت نوع دو', , 10(3), pp. 317-332. doi: 10.22059/jsb.2018.247817.1227
سمایی, سیده سمیه, پیری, مقصود, دلفان, مریم. تأثیر یک دوره تمرین تناوبی شدید و استقامتی تداومی بر بیان ژن‌های آدیپونکتین و TNF-α در رت‌های مبتلا به دیابت نوع دو. , 1397; 10(3): 317-332. doi: 10.22059/jsb.2018.247817.1227

تأثیر یک دوره تمرین تناوبی شدید و استقامتی تداومی بر بیان ژن‌های آدیپونکتین و TNF-α در رت‌های مبتلا به دیابت نوع دو

نشریه علوم زیستی ورزشی
مقاله 4، دوره 10، شماره 3، آذر 1397، صفحه 317-332    اصل مقاله (294.74 K)
نوع مقاله: مقاله پژوهشی Released under CC BY-NC 4.0 license I Open Access I
شناسه دیجیتال (DOI): 10.22059/jsb.2018.247817.1227
نویسندگان
سیده سمیه سمایی1؛ مقصود پیری* 2؛ مریم دلفان3
1دانشجوی دکتری تخصصی فیزیولوژی ورزشی، دانشکدۀ تربیت بدنی و علوم ورزشی، واحد تهران مرکز، دانشگاه آزاد اسلامی، سوهانک، تهران، ایران
2. استاد تمام گروه فیزیولوژی ورزشی، دانشکدة تربیت بدنی و علوم ورزشی، واحد تهران مرکز، دانشگاه آزاد اسلامی، سوهانک، تهران،ایران
3استادیار گروه فیزیولوژی ورزشی، دانشکدة تربیت بدنی و علوم ورزشی، دانشگاه الزهرا، تهران، ایران
چکیده
هدف از پژوهش حاضر مقایسۀ تأثیر یک دوره تمرین تناوبی شدید و استقامتی تداومی بر بیان ژن‌های آدیپونکتین و TNF-α در رت‌های مبتلا به دیابت نوع دو بود. 24 سر رت نژاد ویستار (وزن 10±160 گرم) پس از هفت ماه رژیم غذایی پرچرب و حاوی فروکتوز به سه گروه کنترل، تمرین تناوبی شدید و استقامتی تداومی تقسیم شدند و گروه­های تمرین به مدت هشت هفته/ پنج روز هفته پروتکل تمرینی را اجرا کردند. بیان ژن آدیپونکتین و TNF-α از بافت چربی احشایی همچنین گلوکز و انسولین از پلاسما اندازه‌گیری و شاخص مقاومت به انسولین محاسبه شد. تحلیل داده­ها با از آزمون ANOVA و همبستگی پیرسون انجام گرفت. هر دو مدل تمرینی سبب افزایش معنادار بیان آدیپونکتین شد (016/0=P)، اما بین دو گروه تمرینی اختلاف معناداری نبود (932/0P=)، بیان ژن TNF-α در گروه‌های تمرینی کاهش یافت که در گروه تناوبی بارزتر بود (001/0=P). بین کاهش مقاومت به انسولین و کاهش بیان ژن TNF-α (09/0r=، 001/0P<)و افزایش بیان ژن آدیپونکتین (7/0-r=، 002/0P=) ارتباط معناداری بود. احتمالاً تمرین تناوبی شدید بر تعادل بین عوامل ضدالتهابی و التهابی و بهبود مقاومت انسولینی در رت­های مبتلا به دیابت نوع دو نقشی مؤثر دارد.
کلیدواژه‌ها
آدیپونکتین؛ تمرین استقامتی؛ تمرینات تناوبی شدید؛ دیابت نوع دو؛ TNF-α
عنوان مقاله [English]
The Effect of High Intensity Interval Training and Continuous Endurance Training on Gene Expression of Adiponectin and TNF-α in Type 2 Diabetic Rats
نویسندگان [English]
Seyyedeh Somayeh Samaie1؛ Maghsoud peeri2؛ Maryam Delfan3
1PhD student of Exercise Physiology, Faculty of Physical Education andSport Sciences, TehranUniversity, Tehran, Iran
2Professor, Department of ExercisePhysiology, Faculty of Physical Education and Sport Sciences, Central Tehran Branch, Islamic Azad University, Tehran(Sohanak), Iran
3. Assistant Professor, Department of ExercisePhysiology, Faculty of Physical Education and Sport Sciences,Alzahra University, Tehran, Iran
چکیده [English]
The aim of this study was to compare the effect of high intensity interval training (HIIT) and continuous endurance training (E) on gene expression of adiponectin and TNF-α in type 2 diabetic rats. 24 Wistar rats (weight 160 ± 10 g) were divided into three groups: control, high intensity interval training and continuous endurance training after 7 months of high-fat diet containing fructose. The training groups performed the training protocol for 8 weeks / 5 days a week. The gene expression of adiponectin and TNF-α were measured from visceral fat tissue and glucose and insulin were measured from plasma and insulin resistance index was calculated. Data were analyzed by ANOVA and Pearson correlation. Both types of training significantly increased the expression of adiponectin (P=0.016), but there was no significant difference between the two training groups (P=0.932). The gene expression of TNF-α decreased in training groups and this decrease was more prominent in the HIIT group (P=0.001). There was a significant relationship between reduction of insulin resistance and reduction of TNF-α gene expression (P<0.001, r=0.09) and increase of adiponectin gene expression (P=0.002, r= -0.7). HIIT may have an effective role in the balance between anti-inflammatory and inflammatory factors and regulation of insulin resistance in rats with type 2 diabetes.
کلیدواژه‌ها [English]
Adiponectin, Endurance training, high intensity interval training, TNF-α, type 2 diabetes
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