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کاربرد ریزوباکترهای پروبیوتیک جهت کنترل زیستی ویروس موزاییک توتون در میزبان گوجهفرنگی | |||||
کنترل بیولوژیک آفات و بیماری های گیاهی | |||||
مقاله 10، دوره 7، شماره 2، آذر 1397، صفحه 117-127 اصل مقاله (885.14 K) | |||||
نوع مقاله: مقاله پژوهشی | |||||
شناسه دیجیتال (DOI): 10.22059/jbioc.2019.274441.258 | |||||
نویسندگان | |||||
میلاد آئینی* 1؛ محمد حامد قدوم پاریزی پور2؛ پرنیان پولادی3 | |||||
1گروه گیاه پزشکی، دانشکده کشاورزی، دانشگاه شهید چمران اهواز، اهواز، ایران | |||||
2استادیار گروه گیاهپزشکی، دانشگاه علوم کشاورزی و منابع طبیعی خوزستان، ملاثانی، ایران. | |||||
3دانش آموخته مقطع کارشناسی ارشد بیماری شناسی ، گروه گیاه پزشکی، دانشکده کشاورزی، دانشگاه شهید چمران اهواز، اهواز، ایران. | |||||
چکیده | |||||
ویروس موزاییک توتون (TMV) ازجمله ویروسهایی است که تولید محصول گوجهفرنگی را در مزرعه و گلخانه، بهشدت کاهش میدهد. مطالعه و تحقیق حاضر جهت تعیین ترکیب مناسبی از رایزوباکترهای پروبیوتیک بهمنظور کاهش شدت بیماری ناشی از ویروس موزاییک توتون در میزبان گوجهفرنگی در شرایط گلخانه صورت گرفت. از سه جدایهی باکتری شامل Pseudomonas putida، Pseudomonas fluorescens و Bacillus subtilis پس از اثبات خاصیت عدم آنتاگونیستی با یکدیگر، استفاده شد. غلظت 108 واحد تشکیلدهنده کلنی بر میلیلیتر از سوسپانسیون باکتریها به فراریشهی گیاهچههای گوجهفرنگی اضافه شد. گیاهچهها بهطور مکانیکی با TMV مایهزنی و به مدت شش هفته جهت ظهور علائم نگهداری شدند. دادههای شدت علائم بیماری، غلظت ویروس در گیاهان آلوده و شاخصهای رشدی گیاهان مایهزنی شده ثبت و مورد تجزیهوتحلیل آماری انجام گرفت. نتایج نشان داد که در تیمار ترکیب سه باکتری P. putida، P. fluorescens و B. subtilis شدت علائم و تجمع TMV در گیاهان گوجهفرنگی در مقایسه با سایر تیمارها، کاهش معنیداری دارد. در مقابل، شاخصهای رشدی گیاه با کاربرد این ترکیب باکتریایی، افزایش معنی-داری را در مقایسه با سایرین نشان داد. این نتایج نشان داد که ترکیبی از ریزوباکترهای پروبیوتیک دارای اثر همافزایی بوده و میتواند موجب کاهش غلظت ویروس و افزایش مؤلفههای رشدی گیاه میزبان گردد. | |||||
کلیدواژهها | |||||
الایزای غیرمستقیم؛ مایهزنی؛ چگالی نوری؛ شدت بیماری | |||||
عنوان مقاله [English] | |||||
Management of tobacco mosaic virus by some probiotic bacteria in tomato plant | |||||
نویسندگان [English] | |||||
Milad Aeini1؛ Mohamad Hamed Ghodoum Parizipour2؛ Parnian Pooladi3 | |||||
1Plant Protection Department, Faculty of Agriculture, Shahid Chamran university of Ahvaz, Ahvaz, Iran | |||||
2Assistant Professor, Department of Plant protection, Agricultural Sciences and natural Resources University of Khuzestan, Mollasani. Iran | |||||
3Master of Science in Plant Pathology, Department of Plant Protection, Faculty of Agriculture, Shahid Chamran University of Ahvaz, Ahvaz, Iran. | |||||
چکیده [English] | |||||
Tobacco mosaic virus (TMV) is considered as a devastating virus which significantly reduces the tomato yield. This investigation aimed to find an efficient combination of plant probiotic bacteria to control TMV in tomato. Suspensions of Pseudomonas fluorescens, Pseudomonas putida, and Bacillus subtilis were prepared at the concentration of 108 colony forming unit per milliliter. Tomato seedlings, were treated with probiotic bacteria by adding 100 milliliters of suspension. Afterward, seedlings were inoculated with TMV and were inspected for six weeks. Subsequently, an indirect- ELISA test using TMV specific polyclonal antibody was used to compare the relative virus titer in the plants of each treatment. The experiment was conducted in a completely randomized design with nine treatments and six replicates, respectively. Results showed that the OD in the mixed bacterial treatment was lower (0.08) in comparison to the positive control (0.87) (p<0.05). Application of B. subtilis resulted in the higher OD (0.45) compared to the other treatments (p<0.05). Additionally, plant growth indicators including fresh and dry weight of root and above ground tissues of tomato as well as height of tomato plants and chlorophyl content were recorded. Application of plant probiotic bacteria significantly increased these indices in TMV-inoculated tomato plants compared to the control. The overall results showed that the mixture of three probiotic rhizobacteria had a synergistic effect, so that showed higher decrease in disease severity in comparison to the control. | |||||
کلیدواژهها [English] | |||||
Indirect ELISA, inoculation, Optical density, Disease severity | |||||
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