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khatibi, Mohammad taghi, Mohammad Ganji, shahla, Chavoshei, Somayeh, Kiani, Arda, Hosseini, Seyed Mostafa, Tavallaie, Mahmoud. (1398). SHOX2 and WT1 Promoter Methylation Correlates with the Lung Cancer in Iranian Patients. , 30(2), 113-122. doi: 10.22059/jsciences.2019.71747
Mohammad taghi khatibi; shahla Mohammad Ganji; Somayeh Chavoshei; Arda Kiani; Seyed Mostafa Hosseini; Mahmoud Tavallaie. "SHOX2 and WT1 Promoter Methylation Correlates with the Lung Cancer in Iranian Patients". , 30, 2, 1398, 113-122. doi: 10.22059/jsciences.2019.71747
khatibi, Mohammad taghi, Mohammad Ganji, shahla, Chavoshei, Somayeh, Kiani, Arda, Hosseini, Seyed Mostafa, Tavallaie, Mahmoud. (1398). 'SHOX2 and WT1 Promoter Methylation Correlates with the Lung Cancer in Iranian Patients', , 30(2), pp. 113-122. doi: 10.22059/jsciences.2019.71747
khatibi, Mohammad taghi, Mohammad Ganji, shahla, Chavoshei, Somayeh, Kiani, Arda, Hosseini, Seyed Mostafa, Tavallaie, Mahmoud. SHOX2 and WT1 Promoter Methylation Correlates with the Lung Cancer in Iranian Patients. , 1398; 30(2): 113-122. doi: 10.22059/jsciences.2019.71747

SHOX2 and WT1 Promoter Methylation Correlates with the Lung Cancer in Iranian Patients

Journal of Sciences, Islamic Republic of Iran
مقاله 2، دوره 30، شماره 2، تیر 2019، صفحه 113-122    اصل مقاله (229.87 K)
نوع مقاله: Original Paper
شناسه دیجیتال (DOI): 10.22059/jsciences.2019.71747
نویسندگان
Mohammad taghi khatibi1؛ shahla Mohammad Ganji2؛ Somayeh Chavoshei1؛ Arda Kiani3؛ Seyed Mostafa Hosseini1؛ Mahmoud Tavallaie* 1
1Human Genetic Research Center, Baqiyatallah University of Medical Sciences, Tehran, Islamic Republic of Iran
2Medical Biotechnology Research Institute, National Institute of Genetic Engineering and Biotechnology, Tehran, Islamic Republic of Iran
3Tracheal Research Center, NRITLD, Shahid Beheshti University of Medical Sciences, Tehran, Islamic Republic of Iran
چکیده
Lung cancer is among the most common cause of cancer death in the world. Since the disease is diagnosed in the middle or late stages of the cancer development a more efficient method for an early diagnosis of the disease is required. The main goal of this study was to investigate the correlation between aberrant promoter methylation of the two genes: SHOX2(Short stature homeobox 2) and WT1(Wilms’ tumor gene1), in the plasma samples of the Iranian lung cancer patients. Following filling questionnaires and obtaining the consent of all participants, 70 cases including 45 patients and 25 healthy controls who underwent bronchoscopy procedure in the Masih-Daneshvari Hospital in Tehran, the blood samples were collected. DNA extraction was done and treatment was performed by sodium bisulfite applying Qiagen DNA methylation kit and Methylation specific PCR(MSP) was performed. The results showed a frequency of 82.2% and 55.55% of the hypermethylation for the SHOX2 and WT1 gene promoters in the caces samples, respectively. Our study demonstrated that hypermethylation of SHOX2 and WT1 genes promoter regions might be involved in lung cancer. The methylation of SHOX2 and WT1 in the blood plasma could be considered as the competent biomarkers for an early diagnosis of the lung cancer as a noninvasive method.
کلیدواژه‌ها
Lung cancer؛ MSP؛ SHOX2؛ WT1؛ plasma
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