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کاهش آلودگی نفتی در خاک به روشهای گیاهپالایی، زیست پالایی و گیاهپالایی زیستافزونی شده | ||
تحقیقات آب و خاک ایران | ||
مقاله 211، دوره 50، شماره 10، اسفند 1398، صفحه 2645-2660 اصل مقاله (1.14 M) | ||
نوع مقاله: مقاله پژوهشی | ||
شناسه دیجیتال (DOI): 10.22059/ijswr.2019.278721.668157 | ||
نویسندگان | ||
هادی کوهکن* 1؛ احمد گلچین2؛ محمدصدیق مرتضوی3؛ رقیه همتی4؛ فاطمه شهریاری5 | ||
11. دانشجوی دکتری علوم خاک، دانشکده کشاورزی، دانشگاه زنجان، زنجان، ایران | ||
2استاد گروه علوم خاک، دانشکده کشاورزی، دانشگاه زنجان، زنجان، ایران | ||
3دانشیار پژوهشکده اکولوژی خلیج فارس و دریای عمان، موسسه تحقیقات علوم شیلاتی کشور ،سازمان تحقیقات، آموزش و ترویج کشاورزی، بندرعباس، ایران | ||
4دانشیار گروه گیاهپزشکی، دانشکده کشاورزی، دانشگاه زنجان، زنجان، ایران | ||
5استادیار گروه گیاهپزشکی، دانشکده کشاورزی، دانشگاه زنجان، زنجان، ایران | ||
چکیده | ||
به منظور بررسی کارایی گیاهپالایی، زیستپالایی و گیاهپالایی زیستافزونیشده در حذف نفت خام از خاک، یک آزمایش فاکتوریل در قالب طرح کاملاً تصادفی با سه تکرار به اجرا درآمد. فاکتورها شامل سه سطح آلودگی خاک به نفت (صفر، 4 و 8 درصد وزنی)، چهار تیمار گیاهی (بدون گیاه، برموداگراس (Cynodon dactylon)، سورگوم (bicolorSorghum) و جو (Hordeum vulgare)) و سه تیمار قارچ (بدون قارچ، قارچ بومی و قارچ غیربومی Phanerochate chrysosporium))) بودند. در این تحقیق قارچ جداسازی شده از خاک آلوده از گونه آسپرژیلوس نایجر ( Aspergillus niger) بود. برای انجام آزمایش نمونههای پنج کیلویی خاک با مقادیر مختلف نفت خام آلوده شدند و در گلدانهای پلاستیکی ریخته شدند و پس از گذشت شش هفته با قارچها تلقیح شده و سپس در خاکهای آلوده تلقیح شده با قارچ و تلقیح نشده سه گونه گیاهی گرامینه کاشته شدند. نتایج نشان داد که کاربرد گیاهان سبب افزایش معنیدار درصد حذف نفت خام نسبت به شاهد گردید. درصد حذف نفت خام در خاکهای کشت شده به ترتیب نزولی مربوط به سورگوم، برموداگراس و جو بود. تلقیح خاک با قارچها درصد حذف ترکیبات نفتی را بهطور معنیداری نسبت به شاهد افزایش دادند. قارچ بومی در تجزیه و مصرف مواد نفتی نسبت به قارچ غیربومی مورد مطالعه کارآیی و عملکرد بهتری داشت. همچنین کاربرد توأم آنها (گیاهپالایی زیستافزونیشده) نسبت به کاربرد هر یک از روشها بهتنهایی (گیاهپالایی و زیست پالایی) درصد حذف نفت خام را بطور معنیداری افزایش داد؛ که نشان میدهد کارایی روش گیاهپالایی زیستافزونیشده نسبت به گیاهپالایی و زیستپالایی بهتنهایی بهطور معنیداری بالاتر بود. | ||
کلیدواژهها | ||
آلودگی نفتی؛ زیست افزونی؛ گیاهپالایی | ||
عنوان مقاله [English] | ||
Reduction of Crude Oil Pollution in Soil by Phytoremediation, Bioaugmentation and Bioaugmented Phytoremediation | ||
نویسندگان [English] | ||
Hadi Koohkan1؛ Ahmad Golchin2؛ Mohammad Sedigh Mortazavi3؛ Roghyeh Hemati4؛ Fatemeh Shahryari5 | ||
1Ph. D student, Soil Science Department, Faculty of Agriculture, University of Zanjan, Znajan, Iran | ||
2Professor of Soil Science Department, Faculty of Agriculture, Zanjan University of Zanjan, Zanjan, Iran | ||
3Associate Professor of Persian Gulf and Oman Sea Ecological Research Institute, Iranian fisheries science Research Institute, Agricultural Education and Extension Research Organization, Bandar Abbas, Hormozgan, Iran | ||
4Associate Professor of Plant Protection Department, Faculty of Agriculture, University of Zanjan, Znajan, Iran, | ||
5Assistant Professor of Plant Protection Department, Faculty of Agriculture, University of Zanjan, Znajan, Iran | ||
چکیده [English] | ||
To investigation the efficiency of phytoremediation, bioremediation and bioaugmented phytoremediation to remove crude from the soil, a factorial experiment was conducted in a completely randomized design with three replications. The treatments consisted of 3 levels of oil concentrations in the soil (0, 4 and 8% by weight), four treatments of plant (no plant, bermudagrass (Cynodon dactylon), sorghum (bicolor Sorghum) and barley (Hordeum vulgare)) and 3 treatments of fungi (no fungi, native fungi and Non-native fungi (Phanerochate chrysosporium)). In this study, the isolated fungi from polluted soil was Aspergillus niger. Samples of five kilograms of soil were polluted with different amounts of crude oil and poured into plastic pots. After six weeks, some pots were inoculated with fungi, then all pots were planted with the proposed gramineae species. The results showed that the application of plants significantly increased the removal percentage of crude oil compared to the control. The removal percentage of crude oil was corresponded to cultivated soils of sorghum, bermudagrass, and barley, in desending order. Inoculated soils with fungi increased the removal percentage of crude oil significantly as compared to the control. The native fungus had more efficiency than the non-native fungus in terms of degradation of crude oil. Also, their combined application of bioaugmentation and phytoremediation (bioaugmented phytoremediation) was significantly more than the application of each method alone (phytoremediation and bioremediation); which indicate the better efficiency of the bioaugmented phytoremediation method than the phytoremediation and bioremediation method individually. | ||
کلیدواژهها [English] | ||
Petroleum pollution, Bioaugmentation, Phytoremediation | ||
مراجع | ||
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