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Noruzpour, M., Zare, N., Asghari, R., Sheikhzadeh, P. (2019). Effect of culture media and plant growth regulators on in vitro growth and production of secondary metabolites in Vaccinium arctostaphylos L.. , 50(2), 435-448. doi: 10.22059/ijhs.2018.252894.1415
Mehran Noruzpour; Nasser Zare; Rasool Asghari; Parisa Sheikhzadeh. "Effect of culture media and plant growth regulators on in vitro growth and production of secondary metabolites in Vaccinium arctostaphylos L.". , 50, 2, 2019, 435-448. doi: 10.22059/ijhs.2018.252894.1415
Noruzpour, M., Zare, N., Asghari, R., Sheikhzadeh, P. (2019). 'Effect of culture media and plant growth regulators on in vitro growth and production of secondary metabolites in Vaccinium arctostaphylos L.', , 50(2), pp. 435-448. doi: 10.22059/ijhs.2018.252894.1415
Noruzpour, M., Zare, N., Asghari, R., Sheikhzadeh, P. Effect of culture media and plant growth regulators on in vitro growth and production of secondary metabolites in Vaccinium arctostaphylos L.. , 2019; 50(2): 435-448. doi: 10.22059/ijhs.2018.252894.1415

Effect of culture media and plant growth regulators on in vitro growth and production of secondary metabolites in Vaccinium arctostaphylos L.

علوم باغبانی ایران
Article 17, Volume 50, Issue 2, September 2019, Pages 435-448    PDF (1.21 M)
Document Type: Full Paper
DOI: 10.22059/ijhs.2018.252894.1415
Authors
Mehran Noruzpour1; Nasser Zare* 2; Rasool Asghari3; Parisa Sheikhzadeh4
1M.Sc. Student, Department of Agronomy and Plant Breeding, Faculty of Agriculture and Natural Resources, University of Mohaghegh Ardabili, Ardabil, Iran
2Associate Professor, Department of Agronomy and Plant Breeding, Faculty of Agriculture and Natural Resources, University of Mohaghegh Ardabili, Ardabil, Iran
3Professor, Department of Agronomy and Plant Breeding, Faculty of Agriculture and Natural Resources, University of Mohaghegh Ardabili, Ardabil, Iran
4Assistant Professor, Department of Agronomy and Plant Breeding, Faculty of Agriculture and Natural Resources, University of Mohaghegh Ardabili, Ardabil, Iran
Abstract
The current study was conducted to evaluate the effect of basal culture media and plant growth regulators on establishment and growth of Whortleberry explants and production of secondary metabolites in in vitro conditions. Accordingly, single node explants and apical buds of Whortleberry were collected from forestry areas (Soha, Ardabil, Iran), surface sterilized by benomyl (3 g/l), H2O2 (5%), Ethanol (70%) and sodium hypochlorite (2.5%, pH=10) for 12 min, respectively. The explants were cultured on MS, AN, and WPM media supplemented with 0.1 mg/l NAA or IBA, and different levels of BAP, Zeatin and TDZ. Results showed that growth of explants (%) and number of leaves per explant were affected significantly by type of basal culture medium, plant growth regulators combination and their interaction. Number of leaves per explant and explant’s growth in MS medium was significantly higher than in AN, and WPM media. The highest shoots (%) and number of leaves were obtained in MS basal medium supplemented with 0.1 mg/l IBA plus 0.5 and 1 mg/l Zeatin or 0.5 mg/l BAP. Moreover, there were significant differences among the treatments for secondary metabolite contents. The highest amount of anthocyanin and flavonoid were obtained in MS medium supplemented with 2 mg/l BAP+ 0.1 mg/l IBA and MS medium plus 2 mg/l TDZ and 0.1 mg/l NAA.
Keywords
Basal MS medium; Caucasian whortleberry; In vitro micro-propagation; flavonoids; Medicinal plant
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