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بررسی آلودگی دو سبزی ترب و جعفری به باکتریهای مقاوم به آنتیبیوتیک- مطالعه موردی استان گیلان | ||
| تحقیقات آب و خاک ایران | ||
| مقاله 8، دوره 51، شماره 3، خرداد 1399، صفحه 629-640 اصل مقاله (781.1 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22059/ijswr.2019.290039.668335 | ||
| نویسندگان | ||
| محمدباقر فرهنگی* 1؛ سجاد عبداله زاده2؛ محمود شعبانپور شهرستانی3؛ نسرین قربانزاده2 | ||
| 1گروه علوم خاک، دانشکده کشاورزی، دانشگاه گیلان، رشت، ایران | ||
| 2گروه علوم خاک، دانشگاه گیلان، رشت، ایران | ||
| 3دانشیار گروه خاکشناسى، دانشکده کشاورزى، دانشگاه گیلان، رشت | ||
| چکیده | ||
| کاربرد کود دامی در سبزیکاریها و آبیاری آنها با فاضلاب میتواند مایه رسیدن آنتیبیوتیکها و باکتریهای پایدار در برابر آنها به خاک شود که در پی آن سبزیهای خوراکی به آنها آلوده میشوند. هدف این پژوهش بررسی باکتریهای پایدار در برابر آنتیبیوتیک در دو سبزی ترب و جعفری و خاک زیر کشت آنها بود. سه کشتزار سبزیکاری در شهرهای پیربازار، چابکسر و فومن استان گیلان گزینش شدند. از سبزیها و خاک در سه تکرار نمونهبرداری شد. شمار باکتریهای هتروتروف و کلیفرم آنها پس از ساخت سری رقت، بهترتیب در محیطهای کشت NA و EMB که دارای µg/mL 100 از هر یک از آنتیبیوتیکهای تریمتوپریم، جنتاماسین، سفالکسین و سیپروفلوکساسین بود، شمارش گردید. شناسه پایداری باکتریها به هر آنتیبیوتیک، از تقسیم شمار کلونی‌ها در پتری دارای آن آنتیبیوتیک بر شمار کلونی‌ها در پتری گواه (بدون آن) برآورد شد. دادهها در قالب طرح کرتهای دوبار خرد شده آنالیز شدند. تیمارها شامل جایگاه نمونهبرداری (در سه سطح) به عنوان کرت اصلی، گونه سبزی (در دو سطح) به عنوان کرت فرعی و گونه آنتیبیوتیک (در 4 سطح) کرت فرعی-فرعی بودند. پیامد گونه آنتیبیوتیک بر شناسه پایداری آنتیبیوتیکی باکتریهای سبزیها و خاک معنیدار بود (05/0>p) اما پیامد جایگاه نمونهبرداری و گونه کشت سبزی (ترب و جعفری) به ترتیب تنها بر شناسه پایداری آنتیبیوتیکی باکتریهای هتروتروف و کلیفرم سبزیها معنیدار شدند (01/0>p). در جایگاه چابکسر 29 درصد باکتریهای هتروتروف سبزیها و 42 درصد کلیفرمهای خاک به جنتامایسین پایدار بودند. بالاترین اندازه شناسه پایداری آنتیبیوتیکی کلیفرمها (38 درصد) در سبزی ترب و در برابر جنتامایسین به دست آمد. رویهمرفته باکتریهای قابلکشت سبزیها در جایگاه چابکسر دارای شناسه پایداری به آنتیبیوتیک بالاتری بودند و کاربرد تازهخوری سبزیهای کشت شده در این جایگاه پیشنهاد نمیشود. | ||
| کلیدواژه‌ها | ||
| جنتامایسین؛ چابکسر؛ شاخص مقاومت به آنتیبیوتیک؛ کلیفرمها | ||
| عنوان مقاله [English] | ||
| Investigation of Radish and Parsley Vegetables Contamination to Antibiotic Resistant Bacteria - A Case Study, Guilan Province | ||
| نویسندگان [English] | ||
| Mohammad Bagher Farhangi1؛ Sajjad Abdollahzadeh2؛ Mahmoud Shabanpour3؛ Nasrin Ghirbanzadeh2 | ||
| 1Soil Science Department, Faculty of Agriculture, Guilan University, Rasht, P.O. Box 41635-1314, Iran. | ||
| 2Soil Science Department, University of Guilan, Rasht, Iran | ||
| 3Soil Science Department, University of Guilan, Rasht, Iran | ||
| چکیده [English] | ||
| Manure application and irrigation practice with wastewaters in olericulture may introduce antibiotics and antibiotic resistant bacteria to soil which may contaminate edible vegatables. The aim of this study was to investigate the antibiotic resistant bacteria isolated from radish (Raphanus sativu) and parsley (Petroselinum crispum L.) and their cultivated soils. Three vegetable farms were selected in Pirbazar, Chaboksar and Fouman areas in Guilan province. Samples were taken from vegetables and soils at three replications. After making serial dilution, their heterotrophic and coliform bacteria number were determined on NA and EMB agar media respectively, containing 100 µg/mL antibiotic. Four antibiotics including cephalexin, ciprofloxacin, gentamicin and trimethoprim were tested. Antibiotic resistant index (ARI) was calculated by dividing bacteria colony numbers on each antibiotic contained medium to control (medium without antibiotic). Data were analyzed as split-split plot design with three locations as main plot sector, two olericulture fields as sub-plot and four antibiotics as sub-sub plot factor. The effect of antibiotic was significant on the vegetable and soil bacteria ARI (p<0.05). But the effect of sampling area and farm (radish and parsley farms) were significant only on the heterotrophic and coliform bacteria ARI, respectively (p <0.01). In Chaboksar area, 29% of vegetable's heterotrophic bacteria and 42% of soil coliforms were resistant to gentamicin. The highest coliform ARI (38%) was obtained in radish and was to gentamicin. Overall, culturable bacteria from vegetables of Chaboksar area had higher antibiotic resistance index and eating fresh vegetables cultivated in this area is not recommended. | ||
| کلیدواژه‌ها [English] | ||
| Antibiotic Rsistant Index, Chaboksar, Coliforms, Gentamicin | ||
| مراجع | ||
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