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Ataei Nazari, S., mohammadi sang cheshmeh, A., Bakhtiarizadeh, M., assadi-alamouti, A., Fouladi Nashta, A. (2020). Effect of various concentrations of lipopolysaccharide on in vitro developmental competence of ovine oocyte and metabolites related to the maturation medium. , 22(3), 491-500. doi: 10.22059/jap.2020.290887.623456
sara Ataei Nazari; abdollah mohammadi sang cheshmeh; Mohammad Reza Bakhtiarizadeh; ali assadi-alamouti; Ali Fouladi Nashta. "Effect of various concentrations of lipopolysaccharide on in vitro developmental competence of ovine oocyte and metabolites related to the maturation medium". , 22, 3, 2020, 491-500. doi: 10.22059/jap.2020.290887.623456
Ataei Nazari, S., mohammadi sang cheshmeh, A., Bakhtiarizadeh, M., assadi-alamouti, A., Fouladi Nashta, A. (2020). 'Effect of various concentrations of lipopolysaccharide on in vitro developmental competence of ovine oocyte and metabolites related to the maturation medium', , 22(3), pp. 491-500. doi: 10.22059/jap.2020.290887.623456
Ataei Nazari, S., mohammadi sang cheshmeh, A., Bakhtiarizadeh, M., assadi-alamouti, A., Fouladi Nashta, A. Effect of various concentrations of lipopolysaccharide on in vitro developmental competence of ovine oocyte and metabolites related to the maturation medium. , 2020; 22(3): 491-500. doi: 10.22059/jap.2020.290887.623456

Effect of various concentrations of lipopolysaccharide on in vitro developmental competence of ovine oocyte and metabolites related to the maturation medium

تولیدات دامی
Article 240, Volume 22, Issue 3, September 2020, Pages 491-500    PDF (1.08 M)
Document Type: Research Paper
DOI: 10.22059/jap.2020.290887.623456
Authors
sara Ataei Nazari* 1; abdollah mohammadi sang cheshmeh2; Mohammad Reza Bakhtiarizadeh3; ali assadi-alamouti3; Ali Fouladi Nashta4
1Department animal science, callege aburaihan, tehran university, pakdasht, Iran
2Department Animal science, college aburaihan, tehran university, pakdasht,iran
3Department of Animal and Poultry Science, College of Aburaihan, University of Tehran, Tehran, Iran
4The Royal Veterinary College Royal College Street London NW1 0TU United Kingdom
Abstract
This study was conducted to evaluate the effect of various concentrations of lipopolysaccharide (LPS) in maritaruta medium on oocyte maturation, oocyte developmental competence and metabolites related to maturation medium including glucose, pyruvate, lactate and glutamine. The experimental treatments were 0 (control), 0.01, 0.1, 1 and 10 μg/ml of LPS in oocyte maturation medium. The cumulus oocytes complex (COC) were cultured with various concentrations of lipopolysaccharide. After 24 h of oocyte maturation, the medium was collected and the rates of oocyte maturation, cleaved oocyte and oocytes reached to blastocyst stage were analyzed. Oocyte maturation rate was lowest in the treatment with 10 μg/ml of LPS (P<0.05). Among the measured metabolites, only glucose concentration was linearly
decreased in response to increasing levels of LPS in the maturation medium (P<0.05) as treatment with 10 μg/ml of LPS had lower glucose concentration comparing to other treatments. The percentage of oocyte cleavage was significantly lower in treatment with highest level of LPS compared to other treatments (P<0.05). In addition, the treatment with 1 and 10 μg/ml of LPS significantly reduced blastocyst rate compared to control group (P<0.05). According to results of this study, lipopolysaccharide could have detrimental effects on oocyte development and these influences seems to be mediated through pathways related to energy metabolism. Acquiring managerial approaches to control LPS enhancing agents during reproductive season could prevent animal's reproductive failure.
Keywords
Bacterial cell wall; Ewe; Infectious diseases; Oocyte; Reproductive function
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