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هیدرولیز اسیدی ضایعات نان برای تولید بیواتانول توسط مخمر ساکارومایسس سرویزیه (Saccharomyces cerevisiae) | ||
مهندسی بیوسیستم ایران | ||
مقاله 5، دوره 51، شماره 2، تیر 1399، صفحه 285-293 اصل مقاله (833.29 K) | ||
نوع مقاله: مقاله پژوهشی | ||
شناسه دیجیتال (DOI): 10.22059/ijbse.2020.271766.665133 | ||
نویسندگان | ||
سمانه ترابی1؛ سیدرضا حسن بیگی* 2؛ بهزاد ستاری3؛ برات قبادیان4 | ||
1گروه مهندسی فنی کشاورزی. پردیس ابوریحان، دانشگاه تهران. تهران. ایران. | ||
2گروه مهندسی فنی کشاورزی، پردیس ابوریحان، دانشگاه تهران. تهران. ایران | ||
3گروه مهندسی فناوری صنایع غذایی، پردیس ابوریحان، دانشگاه تهران، تهران، ایران | ||
4گروه مهندسی بیوسیستم، دانشکده کشاورزی، دانشگاه تربیت مدرس، تهران، ایران | ||
چکیده | ||
در حالیکه به میزان زیادی ضایعات نان هر ساله تولید میشود، به دلیل آلودگی به افلاتوکسین، این ضایعات مناسب مصارف غذایی و خوراک دام نیستند. در این پژوهش تولید بیواتانول از ضایعات نان توسط مخمر ساکارومایسس سرویزیه[1] به روش هیدرولیز اسیدی مورد تحقیق قرار گرفت. هیدرولیز اسیدی در دستگاه اتوکلاو انجام شد. اثر غلظت محلول اسیدی و زمان بر میزان گلوکز بررسی گردید. آزمایشها در بارگذاری 160 گرم بر لیتر انجام شد. کاهش آلودگی به آفلاتوکسین برای نمونه با بیشترین بازده در هیدرولیز اسیدی مورد سنجش قرارگرفت. محلول حاصل از هیدرولیز با استفاده از مخمر ساکارومایسس سرویزیه برای تولید بیواتانول استفادهگردید. نتایج نشان داد که اثر متغیرهای غلظت اسید و زمان بر میزان گلوکز در سطح 1% معنیدار است. هیدرولیز در غلظت محلول اسیدی 1 درصد و زمان 20 دقیقه با گلوکز تولیدی 64/80 گرم بر لیتر بیشترین مقدار کربوهیدرات را آزاد میکند. همچنین هیدرولیز اسیدی به ترتیب باعث کاهش100 درصد و 70/20 درصدی آفلاتوکسین B1 و B2شده است. بیشترین درصد تولید بیواتانول در فاز تخمیر نمونههای هیدرولیز، مقدار 7/4 (%v/v) بود. [1]. Saccharomyces cerevisiae | ||
کلیدواژهها | ||
آفلاتوکسین؛ تخمیرزیستی؛ گلوکز؛ زیست سوخت | ||
عنوان مقاله [English] | ||
Acidic Hydrolysis of Bread Waste for Bio-ethanol Production by Saccharomyces Cerevisiae | ||
نویسندگان [English] | ||
Samane Torabi1؛ Seyed Reza Hassan-Beygi2؛ Behzad Sattari3؛ Barat Ghobadian4 | ||
1Dept. Agro-technology, College of Abouraihan, University of Tehran, Tehran, Iran. | ||
2ٍِDept. Agro-technology, College of Abouraihan, University of Tehran, Tehran, Iran | ||
3Dept. Food Industry, College of Aburaihan, University of Tehran, Tehran, Iran | ||
4Dept. of Bio-system Engineering, College of Agriculture, Tarbiat Modares University, Tehran, Iran | ||
چکیده [English] | ||
In-spite of large amounts production of bread waste annually, this waste is not suitable for food and animal feed applications due to aflatoxin contamination. In this study, bio-ethanol production from bread waste by Saccharomyces cerevisiae was investigated using acidic hydrolysis method. The acidic hydrolysis was performed by an autoclave apparatus. The effect of acidic solution concentration and time on amount of glucose was investigated. The experiments were carried out at loading of 160 g/l. Reduction of aflatoxin was measured for the sample with the greatest yield in acid hydrolysis. The sample obtained from hydrolysis was used for the production of bio-ethanol using Saccharomyces cerevisiae. The results showed that the effect of concentration of acid and time was significant at 1% level on the amount of glucose. Hydrolysis liberated the greatest amount of carbohydrate (80.64 g/l glucose) at the acidic solution concentration of 1% and time of 20 minutes. Also, acidic hydrolysis reduced aflatoxin B1 and B2 by 100% and 20.70%, respectively. The greatest percentage of bio-ethanol production in the fermentation phase of hydrolysis samples was 4.7 (v / v%). | ||
کلیدواژهها [English] | ||
Aflatoxin, Bio-fuel, Bio-Fermentation, Glucose | ||
مراجع | ||
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