سامانه پشتیبانی خدمات اطلاعات

  اخبار و اعلانات

 آمار

تعداد نشریات158
تعداد شماره‌ها6,226
تعداد مقالات67,696
تعداد مشاهده مقاله115,006,757
تعداد دریافت فایل اصل مقاله89,688,210
Eshghi Khas, Mostafa, Abbasifar, Ahmadreza, ValizadehKaji, Babak. (1399). Optimization of In Vitro Propagation of Purple Passion Fruit (Passiflora edulis), an Important Medicinal and Ornamental Plant. , 7(3), 305-314. doi: 10.22059/ijhst.2020.297194.342
Mostafa Eshghi Khas; Ahmadreza Abbasifar; Babak ValizadehKaji. "Optimization of In Vitro Propagation of Purple Passion Fruit (Passiflora edulis), an Important Medicinal and Ornamental Plant". , 7, 3, 1399, 305-314. doi: 10.22059/ijhst.2020.297194.342
Eshghi Khas, Mostafa, Abbasifar, Ahmadreza, ValizadehKaji, Babak. (1399). 'Optimization of In Vitro Propagation of Purple Passion Fruit (Passiflora edulis), an Important Medicinal and Ornamental Plant', , 7(3), pp. 305-314. doi: 10.22059/ijhst.2020.297194.342
Eshghi Khas, Mostafa, Abbasifar, Ahmadreza, ValizadehKaji, Babak. Optimization of In Vitro Propagation of Purple Passion Fruit (Passiflora edulis), an Important Medicinal and Ornamental Plant. , 1399; 7(3): 305-314. doi: 10.22059/ijhst.2020.297194.342

Optimization of In Vitro Propagation of Purple Passion Fruit (Passiflora edulis), an Important Medicinal and Ornamental Plant

International Journal of Horticultural Science and Technology
دوره 7، شماره 3، آذر 2020، صفحه 305-314    اصل مقاله (739.58 K)
نوع مقاله: Research paper
شناسه دیجیتال (DOI): 10.22059/ijhst.2020.297194.342
نویسندگان
Mostafa Eshghi Khas؛ Ahmadreza Abbasifar* ؛ Babak ValizadehKaji
Department of Horticultural Sciences, Faculty of Agriculture and Natural Resources, Arak University, 38156-8-8349 Arak, Iran
چکیده
Tissue culture techniques can be beneficial for quick clonal propagation and production of disease-free plants of purple passion fruit as one of the most important medicinal and ornamental plants. These techniques are essential tools for the production of transgenic plants and high-value phytochemicals. The present study was planned to introduce an efficient in vitro propagation for purple passion fruit(Passiflora edulis Sims.). To do so, the effect of different plant growth regulators was investigated on micropropagation medium of purple passion fruit. For proliferation stage, nodal segments were cultured in media supplemented with various combinations of different plant growth regulators including: BA (0, 2.2, 4.4 and 8.9 μM), TDZ (2.3, 4.5 and 9.1 μM), GA3 (0 and 2.9 μM) and IBA (0 and 0.5 μM).  For rooting, shoots with about 1.5 cm long originating from explants were removed and cultured in half-strength MS medium containing different concentrations of auxin-based plant growth regulators including: IAA (0, 1.1, 2.9, 5.7 and 11.4 μM), IBA (1, 2.5, 4.9 and 8.9 μM), and NAA (1.1, 2.7, 5.4, 10.7 μM). For the proliferation stage, the best plant growth regulator combination was 8.9 μM BA+2.9 μM GA3+0.5 μM IBA, resulting in the maximum shoot proliferation, number of shoots per explants, and shoot length. Half-strength MS medium supplemented with 5.4 μM NAA or 8.9 μM IBA was the most effective treatment for the rooting of shoots. Gradual acclimatization of the rooted plantlets was performed and the plantlets were established in the soil successfully. The micropropagated plants did not exhibit any visually detectable variation to their mother plants.
کلیدواژه‌ها
Nodal explant؛ Plant growth regulator؛ Proliferation؛ Rooting
مراجع
  1. Aldwinckle H, Malnoy M. 2009. Plant regeneration and transformation in the Rosaceae. In: Nageswara-Rao M, and Soneji J R (Eds.) Transgenic plant. Special Issue 1, pp 1-39.
  2. Anand S.P, Jayakumar E, Jeyachandran R, Nandagobalan V, Doss A. 2012. Direct organogenesis of Passiflora foetida L. through nodal explants. Plant Tissue Culture and Biotechnology 22, 87–91.
  3. Busilacchi H, Severin C, Gattuso M, Aguirre A, Di Sapio O, Gattuso S. 2008. Field culture of micropropagated Passiflora caeruela L. histological andchemical studies. B. Latinoam. Caribe Pl 7:257–263.
  4. da Silva C.V, Loriato V.A.P, Oliveira L.S, Otoni W.C. 2011. Efeito dos brassinosteroides e da 6-benzilaminopurina na organogenese in vitro de Passiflora cincinnata Mast. XIII Congresso Brasileiro de fisiologia vegetal XIV Reuniao Latino-Americana de fisiologia vegetal do gene a planta. Buzios, Brasil.
  5. De Klerk G.J, Arnholdt-Schmitt B, Lieberei R, Neumann K.H. 1997. Regeneration of roots, shoots and embryos: physiological, biochemical and molecular aspects. Biologia Plantarum 39, 53-66.
  6. Garcia R, Pacheco G, Falcão E. 2011. Influence of type of explant, plant growth regulators, salt composition of basal medium, and light on callogenesis and regeneration in Passiflora suberosa L. (Passifloraceae). Plant Cell Tissue and Organ Culture 106, 47-54.
  7. George E.F, Hall M.A, De Klerk G.J. 2008. Plant Propagation by Tissue Culture. Vol. 1. 3th Ed. Dordrecht, The Netherlands, Springer.
  8. Huetteman C.A, Preece J.E. 1993. Thidiazuron: a potent cytokinin for woody plant tissue culture. Plant Cell Tissue and Organ Culture 33, 105–119.
  9. Huh Y.S, Lee J.K, Nam S.Y. 2017. Effect of plant growth regulators and antioxidants on in vitro plant regeneration and callus induction from leaf explants of purple passion fruit (Passiflora edulis Sims). Journal of Plant Biotechnology 44, 335–342.

10. Iapichino G, Airò M. 2009. Multiplication of Crataegus monogyna by in vitro culture of nodal segments, Acta Horticulturae 812, 135–140.

11. Krishna H, Singh N.K. 2007. Biotechnological advances in mango Mangifera indica L. and their future application in crop improvement: a review. Biotechnology Advances 25, 223–243.

12. Murashige T, Skoog F. 1962 . A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum 15, 473–497.

13. Naik S.K, Chand P.K. 2011. Tissue culture-mediated biotechnological intervention in pomegranate: a review. Plant Cell Reports 30, 707–721.

14. Nakasone H.Y, Paull R.E. 1998. Tropical Fruits. CAB International, Wallingford, UK.

15. Ning G.G, Bai S.P, Bao M.Z, Liu L. 2007. Factors affecting plantlet regeneration from in vitro cultured immature embryos and cotyledons of Prunus mume “Xue mei”. In Vitro Cellular & Developmental Biology-Plant 43(3), 225–230.

16. Ozarowski M, Błaszkiewicz S, Gryszczynska A, Thiem B, Budzianowski J. 2012. Search for C-glycosyl flavones and phenolic acids in callus and shoot in vitro culture of Passiflora caerulea L. International conference: Business meets science to cooperate in current topics. Bioconnect. Poznan, Poland.

17. Ozarowski M, Sedzik K, Gryszczynska A, Thiem B. 2013. Optimization of conditions for in vitro propagation of valuable medicinal plants of Passiflora incarnata L. and P. caerulea L. International conference: “Facilitating dialogue between business and academia”. Bioconnect. Poznan, Poland.

18. Ozarowskia M, Thiema T. 2013. Progress in micropropagation of Passiflora spp. to produce medicinal plants: a mini-review. Revista Brasileira de Farmacognosia 23, 937–947.

19. Pinto A.P.C, Monteiro-Hara A.C.B.A, Stipp L.C.L, Mendes B.M.J. 2010. In vitro organogenesis of Passiflora alata. In Vitro Cellular & Developmental Biology-Plant 46, 28–33.

20. Prithviraj H.S, Hemanth Kumar N.K, Prakasha. Shobha J. 2015. An efficient in vitro regenaretion of multiple shoot from leaf explant of Passiflora caerulea L. an important medicinal plant. International Journal of Recent Scientific Research 6(11), 7263–726.

21. Ragavendran C, Kamalanathan D, Reena G, Natarajan D. 2012. In vitro propagation of nodal and shoot tip explants of Passiflora foetida L. An exotic medicinal plant. Asian Journal of Plant Science and Research 2, 707–711.

22. Rai M.K, Asthana P, Jaiswal V.S, Jaiswal U. 2010. Biotechnological advances in guava (Psidium guajava L.): recent developments and prospects for further research. Trees - Structure and Function 24, 1–12.

23. Rai M.K, Jaiswal V.S, Jaiswal U. 2009. Shoot multiplication and plant regeneration of guava Psidium guajava L. from nodal explants of in vitro raised plantlets. Journal of Fruit and Ornamental Plant Research 17, 29–38.

24. Reis L.B, Paiva Neto V.B, Toledo Picoli E.A, Costa M.G.C, Rego M.M, Carvalho C.R, Finger F.L, Otoni W.C. 2003. Axillary bud development of passion fruit as affected by ethylene precursor and inhibitors. In Vitro Cellular & Developmental Biology-Plant 39, 618–622.

25. Shukla S, Shukla S.K, Mishra S.K. 2008. In vitro plant regeneration from seedling explants of Stereospermum personatum D.C.: a medicinal tree. Trees - Structure and Function 23, 409–413.

26. Silva G.M, Cruz A.C.F, Otoni W.C, Pereira T.N.S, Rocha D.I, Silva M. 2015. Histochemical evaluation of induction of somatic
embryogenesis in Passiflora edulis Sims (Passifloraceae). In Vitro Cellular & Developmental Biology-Plant 51, 539–545.

27. ValizadehKaji B, Ershadi A, Tohidfar M. 2013. In vitro propagation of two Iranian commercial pomegranates (Punica granatum L.) cvs. ‘Malas Saveh’ and ‘Yusef Khani’. Physiology and Molecular Biology of Plants 19(4), 597–603.

28. Vieira L.M, Rocha D.I, Taquetti M.F, da Silva L.C, De Campos J.M.S, Viccini L.F, Otoni W.C. 2014. In vitro plant regeneration of Passiflora setacea D.C. (Passifloraceae): the influence of explant type, growth regulators, and incubation conditions. In Vitro Cellular & Developmental Biology-Plant 50(6), 738-745.

آمار
تعداد مشاهده مقاله: 886
تعداد دریافت فایل اصل مقاله: 1,100


سامانه مدیریت نشریات علمی. قدرت گرفته از سیناوب