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Immobilized lysozyme onto bacterial cellulose nanofibers as active and reinforcing agent of sodium caseinate based films: physical characteristics and antimicrobial activity | ||
| Journal of Food and Bioprocess Engineering | ||
| مقالات آماده انتشار، پذیرفته شده، انتشار آنلاین از تاریخ 22 شهریور 1399 | ||
| نوع مقاله: Original research | ||
| شناسه دیجیتال (DOI): 10.22059/jfabe.2020.308532.1062 | ||
| نویسندگان | ||
Pashew Bayazidi1؛ Hadi Almasi  2؛ Behbood Purfathi3
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| 1Q.C and Analyzer of Samin Laboratory, Urmia, Iran | ||
| 2Department of food science and technology, Faculty of agriculture, Urmia University | ||
| 3R&D department of Shirin Asal food industry, Tabtiz, Iran | ||
| چکیده | ||
| The aim of this research was to compare the effect of free and immobilized Lysozyme on the physicochemical and antimicrobial properties of sodium caseinate (SC) based active films. Lysozyme was immobilized onto bacterial cellulose nanofiber (BCNF) and decreasing of its activity was approved after immobilization. Free and immobilized enzymes were incorporated into SC films at the concentrations of 0.5 and 1 mg.100ml-1 and the films were characterized. Addition of BCNF and lysozyme diminished significantly (p˂0.05) the moisture absorption and water vapor permeability of SC films and immobilized enzyme had higher effect than free enzyme on decreasing of these parameters. The tensile strength and Young’s modulus were increased and elongation to break was decreased by incorporation of BCNF. An adverse effect was observed for lysozyme addition but the effect of immobilized enzyme on the weakening of tensile properties was lower than free lysozyme. According to XRD results, the crystallinity of films increased by incorporation of BCNF and lysozyme. However, the immobilization reduced the crystalline regions of BCNF. Antimicrobial activity of lysozyme increased after immobilization and SC films containing immobilized enzyme exhibited considerable activity against Gram-positive bacteria S.aureus, L.monocytogenes. Gram-negative bacteria E.coli, Y.enterocolitica, mold of A.niger and yeast of S.cerevisiae. | ||
| کلیدواژهها | ||
| Active film؛ Lysozyme immobilization؛ Sodium caseinate؛ Physical properties؛ Antimicrobial activity | ||
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آمار تعداد مشاهده مقاله: 161 |
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