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بررسی پاسخ‌های آنتی اکسیداسیونی برای ریشه یک موتانت برنج تحت تنش شوری در مرحله گیاهچه‌ای | ||
| به زراعی کشاورزی | ||
| مقاله 1، دوره 23، شماره 1، فروردین 1400، صفحه 1-14 اصل مقاله (568.81 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22059/jci.2020.288721.2266 | ||
| نویسندگان | ||
| مریم فروغ1؛ سعید نواب پور* 2؛ اسماعیل ابراهیمی3؛ علی اکبر عبادی4؛ داود کیانی5 | ||
| 1دانشجوی کارشناسی‌ارشد، گروه اصلاح نباتات و بیوتکنولوژی، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان، ایران. | ||
| 2دانشیار، گروه اصلاح نباتات و بیوتکنولوژی، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان، ایران | ||
| 3دانشیار، پژوهشکده بیوتکنولوژی، دانشگاه شیراز، شیراز، ایران | ||
| 4استادیار، مؤسسه تحقیقات برنج ایران، سازمان آموزش و توسعه تحقیقات کشاورزی رشت، رشت، ایران. | ||
| 5ستادیار پژوهش، بخش تحقیقات اصلاح و تهیه نهال و بذر، مرکز تحقیقات و آموزش کشاورزی و منابع طبیعی استان بوشهر، سازمان تحقیقات، آموزش و ترویج کشاورزی | ||
| چکیده | ||
| در این مطالعه به‌منظور بررسی پاسخ به تنش شوری، گیاهچههای برنج دو رقم هاشمی و لاین پیشرفته موتانت آن در آزمایشی در سال 1396 در دانشگاه علوم کشاورزی و منابع طبیعی گرگان تحت شرایط تنش شوری 100 میلیمولار نمک کلریدسدیم قرار داده شدند. نمونه‌برداری از ریشه در زمانهای سه و شش روز پس از تنش شوری برای بررسیهای بیوشیمیایی انجام گرفت. آزمایش به‌صورت کرتهای خردشده با طرح پایه بلوک‌های کامل تصادفی با سه تکرار به‌صورت کشت هیدروپونیک اجرا گردید. در شرایط تنش شوری محتوای یون سدیم در ریشه هر دو ژنوتیپ به‌شدت افزایش یافت، اما این افزایش در ریشه ژنوتیپ موتانت نسبت به والد غیرموتانت به‌طور معنیداری کم‌تر بود. تنش اکسیداتیو حاصل از تنش شوری با اندازهگیری میزان پراکسید هیدروژن مشخص کرد که رقم والد تحت تنش اکسیداتیو بیش‌تری بوده است و این نتیجه با میزان بیش‌تر مالون‌دی‌آلدهید مورد تأیید قرار گرفت. ارزیابی فعالیت آنزیمهای آنتی‌اکسیداسیونی سوپراکسیددیسموتاز، کاتالاز، آسکوربات‌پراکسیداز و گلوتاتیون‌ردوکتاز نشان از افزایش معنی‌دار این آنزیمها در ریشه ژنوتیپ موتانت داشت. به‌طور کلی، نتایج این پژوهش نشان داد که در ژنوتیپ برنج موتانت، جهش (با پرتودهی گاما) با افزایش فعالیت آنزیمهای اکسیداسیونی و سنتز برخی اسمولیتها در بافت ریشه، باعث افزایش تحمل به شوری در مرحله گیاهچه‌ای نسبت به والد غیرموتانت شد. | ||
| کلیدواژه‌ها | ||
| اسمولیت؛ جهش؛ فعالیت آنزیمی؛ کلرید سدیم؛ مالون دی‌آلدهید | ||
| عنوان مقاله [English] | ||
| Assessment of antioxidant responses of a mutant rice root under salinity stress in the seedling stage | ||
| نویسندگان [English] | ||
| Maryam Forough1؛ Saeid Navabpour2؛ Esmaeil Ebrahimie3؛ Ali Akbar Ebadi4؛ Davood Kiani5 | ||
| 1M.Sc. Student, Plant Breeding and Biotechnology Department, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran. | ||
| 2Associate Professor, Plant Breeding and Biotechnology Department, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran | ||
| 3Associate Professor, Biotechnology Research Center, Shiraz University, Shiraz, Iran | ||
| 4Assistant Professor, Rice Research Institute of Iran (RRII), Agricultural Research Education and Extension Organization (AREEO) Rasht, Rasht, Iran. | ||
| 5Research Assistant Professor, Seed and Plant Improvement Research Department, Bushehr Agricultural and Natural Resources Research and Education Center, AREEO, Bushehr, Iran. | ||
| چکیده [English] | ||
| In this study, in order to investigate the response to salinity stress, seedlings of two rice kinds, Hashemi and its advanced mutant line, have exposed to 100 mM NaCl as a salinity stress in an experiment, conducted in 2017 at Gorgan University of Agricultural Sciences and Natural Resources, Iran. For the biochemical investigation, root sampling is performed during three and six days after the salinity stress treatment. The experiment is conducted as a split plot with randomized complete block design with three replications in hydroponic culture. Under salinity stress, the sodium ion content in both genotypes’ roots has increased significantly, while this trend is much lower in the root of mutant genotype than wild type. The induced oxidative stress of salinity stress is measured by the amount of hydrogen peroxide, indicating that the wild type is under higher oxidative stress which is confirmed by the higher amount of malondialdehyde. Evaluation of antioxidant enzymes’ activity include superoxide dismutase, catalase, ascorbate peroxidase, and glutathione reductase. It reveals a significant rise in the root of the mutant genotype. Overall, this study shows that mutation in the rice genotype leads to salt tolerance, compared to the wild type, through promoting the activity of oxidative enzymes and the synthesis of some osmolytes in the root tissue. | ||
| کلیدواژه‌ها [English] | ||
| Enzyme activity, Malondialdehyde, Mutation, Osmolytes, Sodium chloride | ||
| مراجع | ||
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