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Ghasemi, Omid, Karami, Fatemeh, Salahshourifar, Iman, Nooridaloii, Mohammad. (1400). Expression Analysis of and Genes in Oral Squamous Cell Carcinoma Patients. , 32(2), 115-120. doi: 10.22059/jsciences.2020.310939.1007576
Omid Ghasemi; Fatemeh Karami; Iman Salahshourifar; Mohammad Reza Nooridaloii. "Expression Analysis of and Genes in Oral Squamous Cell Carcinoma Patients". , 32, 2, 1400, 115-120. doi: 10.22059/jsciences.2020.310939.1007576
Ghasemi, Omid, Karami, Fatemeh, Salahshourifar, Iman, Nooridaloii, Mohammad. (1400). 'Expression Analysis of and Genes in Oral Squamous Cell Carcinoma Patients', , 32(2), pp. 115-120. doi: 10.22059/jsciences.2020.310939.1007576
Ghasemi, Omid, Karami, Fatemeh, Salahshourifar, Iman, Nooridaloii, Mohammad. Expression Analysis of and Genes in Oral Squamous Cell Carcinoma Patients. , 1400; 32(2): 115-120. doi: 10.22059/jsciences.2020.310939.1007576

Expression Analysis of and Genes in Oral Squamous Cell Carcinoma Patients

Journal of Sciences, Islamic Republic of Iran
مقاله 2، دوره 32، شماره 2، مرداد 2021، صفحه 115-120    اصل مقاله (412.2 K)
نوع مقاله: Original Paper
شناسه دیجیتال (DOI): 10.22059/jsciences.2020.310939.1007576
نویسندگان
Omid Ghasemi1؛ Fatemeh Karami2؛ Iman Salahshourifar1؛ Mohammad Reza Nooridaloii* 3
1Department of Biology, School of Basic Science, Science and Research Branch, Islamic Azad University, Tehran, Islamic Republic of Iran
22 Department of Medical Genetics, Applied Biophotonics Research Center, Science and Research Branch, Islamic Azad University, Tehran, Islamic Republic of Iran
3Department of Medical Genetics, School of Medicine, Tehran University of Medical Sciences, Tehran, Islamic Republic of Iran
چکیده
Oral squamous cell carcinoma (OSCC) is still one of the leading cancer related death, worldwide. Owing to the critical role of ALDH1 isoforms in pathogenesis of cancers and controversies in previous reports, the present study was conducted to determine the expression of two challenging ALDH1 isoforms in OSCC tissue samples. RNA was isolated from 30 OSCC and normal fresh tissues margins and then was converted to cDNA using Thermo Scientific™ Fermentas cDNA synthesis kit. Reverse Transcription Real time polymerase chain reaction (RT-Real time PCR) was performed on synthesized cDNA using primer pairs specific to ALDH1A1 and ALDH1A3 genes. It was found that the ALDH1A1 mRNA expression was significantly higher in OSCC samples compared to normal margins (P-value= 0.001). ALDH1A1 and ALDH1A3 expression was significantly associated with alcohol drinking (P-value= 0.01) and smoking (P-value=0.008), respectively. There was no correlation among ALDH1A1 and ALDH1A3 expressions and clinicopathological features (p-value>0.05). Confirmation of the over-expression ofALDH1A1 isoformin OSCC samples maybe indicating that it plays critical role in pathogenesis of OSCC. However, owing to the discrepant findings on the association of ALDH1A1 expression with clinicopathological features, further studies are required to identify its role as driver or passenger carcinogenic alteration and its exact association with smoking.
کلیدواژه‌ها
Gene expression؛ Oral squamous cell carcinoma
سایر فایل های مرتبط با مقاله
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