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ارزیابی واکنش های رشدی، خصوصیات بیوشیمیایی، ظرفیت تبادلات گازی و عملکرد فتوسیستم II گیاه سیاه دانه تحت تیمار نانوذره نقره | ||
| به زراعی کشاورزی | ||
| مقاله 12، دوره 23، شماره 4، دی 1400، صفحه 870-855 اصل مقاله (1.42 M) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22059/jci.2021.307346.2427 | ||
| نویسندگان | ||
| سارا بنی ابراهیم1؛ لیلا پیشکار* 2؛ علیرضا ایرانبخش3؛ داریوش طالعی4؛ گیتی برزین2 | ||
| 1دانشجوی دکتری، گروه زیست‌شناسی، واحد اسلامشهر، دانشگاه آزاد اسلامی، اسلامشهر، ایران. | ||
| 2استادیار، گروه زیست‌شناسی، واحد اسلامشهر، دانشگاه آزاد اسلامی، اسلامشهر، ایران. | ||
| 3استاد، گروه زیست‌شناسی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران. | ||
| 4استادیار، مرکز تحقیقات گیاهان دارویی، دانشگاه شاهد، تهران، ایران. | ||
| چکیده | ||
| بهمنظور بررسی پاسخهای فیزیولوژیکی گیاه دارویی سیاه‌دانه تحت غلظتهای مختلف نانوذره نقره (صفر، 5/2، 5، 10، 20، 40 و 80 میلیگرم بر لیتر)، آزمایشی در قالب طرح کاملاً تصادفی با پنج تکرار در گلخانه دانشگاه شاهد در سال 1397 انجام شد. صفات رشدی، رنگیزههای فتوسنتزی، آنتوسیانین، محتوای پرولین، گلایسین بتائین، قندهای محلول و نامحلول، ترکیبات فنلی و پارامترهای تبادلات گازی و فلورسانس دستگاه فتوسنتزی در پژوهش حاضر موردبررسی قرار گرفتند. نتایج نشان داد که تیمار نانوذره نقره در غلظتهای بالا (20، 40 و 80 میلیگرم بر لیتر) به‌طور معنی‌داری از تولید زیست‌توده و رشد ریشه و ساقه ممانعت کرد و باعث کاهش رنگیزههای کلروفیل a و b شد، درحالی‌که تیمار نانوذره نقره محتوای کاروتنوئیدها، پرولین، گلایسین بتائین، قندهای محلول، آنتوسیانین و ترکیبات فنلی را نسبت به شاهد افزایش داد. با افزایش غلظت نانوذره نقره، نسبت فتوسنتز، نسبت تعرق، هدایت روزنهای، ماکسیمم عملکرد کوانتومی فتوسیستمII، ضریب خاموشی فتوشیمیایی و عملکرد کوانتومی مؤثر فتوشیمیایی فتوسیستمII کاهش یافت. بررسی فلورسانس کلروفیل a نشان داد غلظتهای بالای نانوذره نقره باعث ممانعت انتقال انرژی از کمپلکس دریافتکننده نور به مرکز واکنش، تخریب کمپلکس تجزیهکننده آب در فتوسیستمII و غیرفعال‌شدن مرکز واکنش فتوسیستمII شد. نتایج کلی نشان داد که تیمار با نانوذره نقره باعث القای مکانیسم بازدارندگی بر فرایند فتوسنتز و در نتیجه، تولید زیست‌توده گیاه سیاه‌دانه شد. | ||
| کلیدواژه‌ها | ||
| تبادلات گازی؛ ترکیبات اسمولیت؛ سیاه دانه؛ فلورسنس کلروفیل؛ نانو ذره نقره | ||
| عنوان مقاله [English] | ||
| Evaluation of growth, biochemical parameters, gas exchange capacity and photosystem II performance responses in black cumin plants under silver nanoparticles treatment | ||
| نویسندگان [English] | ||
| Sara Baniebrahimi1؛ Leila Pishkar2؛ Alireza Iranbakhsh3؛ Daryush Talei4؛ Giti Barzin2 | ||
| 1Ph.D. Candidate, Department of Biology, Islamshahr Branch, Islamic Azad University, Islamshahr, Iran. | ||
| 2Assistant Professor, Department of Biology, Islamshahr Branch, Islamic Azad University, Islamshahr, Iran. | ||
| 3Professor, Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran. | ||
| 4Assistant Professor, Medicinal Plants Research Center, Shahed University, Tehran, Iran. | ||
| چکیده [English] | ||
| In order to investigate the physiological responses of black cumin seed (Nigella sativa L.) under different concentrations of silver nanoparticles (0, 2.5, 5, 10, 20, 40, and 80 mg/L AgNPs), a completely randomized design experiment is conducted with 5 replications in the greenhouse of Shahed University in 2018. In the present study, growth traits, photosynthetic pigments, anthocyanins, proline content, glycine betaine, soluble and insoluble sugars, phenolic compounds and gas exchange, and fluorescence parameters of the photosynthetic apparatus are investigated. The results show that the AgNPs treatments significantly inhibit biomass production and the growth of root and shoot, decreasing the contents of chlorophyll a and b at high concentrations (20, 40 and 80 mg/L), while the AgNPs treatments increase the content of carotenoids, proline, glycine betaine, soluble sugars, anthocyanins, and phenolic compounds, compared to the control. By increasing the concentration of AgNPs, photosynthetic rate, transpiration rate, stomatal conductance, the maximal quantum yield of PSII photochemistry, photochemical quenching coefficient, and effective quantum yield of PSII photochemistry decline. Measurement of fluorescence show strong evidence of inhibitory effects on energy transfer from light harvesting complexes to reaction centers, the deterioration of the PSII water splitting system and the inactivation of PSII reaction centers at high concentrations of AgNPs. In conclusion, the results demonstrate that AgNPs induce an inhibitory mechanism on photosynthetic processes and biomass of black seed plants. | ||
| کلیدواژه‌ها [English] | ||
| Black cumin, Chlorophyll fluorescence, Compatible solutes, Gas exchange, Silver nanoparticle | ||
| مراجع | ||
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