پیوندهای مفید
اخبار و اعلانات
آمار
| تعداد نشریات | 161 |
| تعداد شمارهها | 6,473 |
| تعداد مقالات | 69,969 |
| تعداد مشاهده مقاله | 122,749,894 |
| تعداد دریافت فایل اصل مقاله | 95,899,597 |
An Efficient In Vitro Propagation Protocol of Pot Calla Lily (Zantedeschia spp cv. Orania and Sunclub) Via Tuber Production | ||
| International Journal of Horticultural Science and Technology | ||
| دوره 8، شماره 4، دی 2021، صفحه 343-351 اصل مقاله (1.49 M) | ||
| نوع مقاله: Research paper | ||
| شناسه دیجیتال (DOI): 10.22059/ijhst.2021.317458.436 | ||
| نویسندگان | ||
| Elaheh Hashemidehkordi1؛ Seyed Najmmaddin Mortazavi1؛ Pejman Azadi* 2 | ||
| 1Department of Horticulture Science, Faculty of Agriculture, University of Zanjan, Iran | ||
| 2Department of Genetic Engineering and Biosafety, Agricultural Biotechnology Research Institute of Iran (ABRII), Karaj, Agricultural Research, Education and Extension Organization (AREEO), Iran. Department of Tissue Culture, Ornamental Plants Research Center, Horticultural Science Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Mahallat, Iran | ||
| چکیده | ||
| Zantedeschia spp is an important flower in the ornamental plants market. Due to the high demand for this plant in the horticultural industry, it is indispensable to introduce an in vitro protocol for its mass propagation. For this aim, the tubers of calla lily were disinfected in a hot water bath with different temperatures (30, 35, 40, 45, and 50 oC) and duration (30 or 35 min). Then explants were cultured on MS medium with different combinations of 6-Benzylaminopurine (BAP) and Kinetin (Kin). Based on the obtained results, the highest disinfection percentage (more than 90%) was obtained at 45 oC for 35 min. Also, the highest proliferation rate (with an average of 15.33 and 14.32 in cv. Orania and cv. Sunclub, respectively) was observed in 2.5 mg L-1 BAP + 1.5 mg L-1 Kin. The highest rooting percentage (100% in both cultivars) and root number per explant (with an average of 4.00 and 3.03 in cv. Orania and Sunclub, respectively) was obtained in 0.5 mg L-1 Indole-3-acetic acid (IAA) + 0.1 mg L-1 Kin, but the highest root length (with an average of 120.0 and 106.6 mm in cv. Orania and Sunclub, respectively) was observed in 1.0 mg L-1 IAA + 0.1 mg L-1 Kin. In MS medium + 2.0 mg L-1 Indole-3-butyric acid (IBA) + 4% Sucrose, the highest number of tubers (with an average of 6.66 and 5.21 in cv. Orania and Sunclub, respectively) was formed. The highest fresh and dry weights (with an average of 948.33 and 851.33 mg in cv. Orania and Sunclub, respectively) of tuber were obtained in 2.0 mg L-1 IBA + 6% sucrose. The rooted and tuberous plants were adapted in the greenhouse successfully. | ||
| کلیدواژهها | ||
| Bulbous plant؛ Disinfection؛ Micropropagation؛ Sucrose | ||
| مراجع | ||
|
Altan F, Burun B, Sahin N. 2010. Fungal contaminants observed during micropropagation of Lilium candidum L. and the effect of chemotherapeutic substances applied after sterilization. African Journal of Biotechnology 9, 991–995. Altindal D, Karadogan T. 2010. The effect of carbon sources on in vitro microtuberization of potato (Solanum tuberosum L.). Turkish Journal of Field Crops 15, 7-11. Asayesh ZM, Vahdati K, Aliniaeifard S, Askari N. 2017. Enhancement of ex vitro acclimation of walnut plantlets through modification of stomatal characteristics in vitro. Scientia Horticulturae 220, 114-121. Asayesh ZM, Vahdati K, Aliniaeifard S. 2021. Stomatal morphology and desiccation response of persian walnut tissue culture plantlets influenced by the gelling agent of in vitro culture medium. Journal of Nuts 12 (1), 41-52. Askari N, Visser R, De Klerk G. 2018. Growth of lily bulblets in vitro, a review. International Journal of Horticultural Science and Technology 5(2), 133-143. doi: 10.22059/ijhst.2018.268870.263 Azadi P, Bagheri H, Molaahmad Nalousi A, Nazari F, Chandler S.F. 2016. Current status and biotechnological advances in genetic engineering of ornamental plants. Biotechnology Advances 34, 1073-1090. Azadi P, Khosh-Khui M. 2007. Micropropagation of Lilium ledebourii (Baker) Boiss as affected by plant growth regulator, sucrose concentration, harvesting season and cold treatments. Electronic Journal of Biotechnology 10, Bairu M.W, Kane M.E. 2011. Physiological and developmental problems encountered by in vitro cultured plants. Plant growth regulators 63, 101-103. Baker C.M, Wetzstein H.Y. 2004. Influence of auxin type and concentration on peanut somatic embryogenesis. Plant Cell Tissue and Organ Culture 36, 361-368. Cardoso J.C, Teixeira da Silva J.A. 2012. Micropropagation of gerbera using chlorine dioxide (ClO2) to sterilize the culture medium. In Vitro Cellular & Developmental Biology 48, 362–8. Chang H.S, Chakrabarty D, Hahn E.J, Paek K.Y. 2003. Micropropagation of calla lily (Zantedeschia albomaculata) via in vitro shoot tip proliferation. In Vitro Cellular & Developmental Biology 39, 129–134. Chen J.J, Liu M.C, Ho Y.H. 2000. Size of In Vitro Plantlets Affects Subsequent Tuber Production of Acclimated Calla Lily. Hortscience 35, 290–292. Deloire A, Charpentier M, Berlioz G, Colin A, Gimonnet G. 1995. Micropropagation of the grapevine: Results of 10 years of experiments in the chmpagne vineyard and results of the first vinifications. American Journal of Enology and Viticulture 46, 571-580. Ebrahim M.K.H. 2004. Comparison, determination and optimizing the conditions required for rhizome and shoot formation, and flowering of in vitro cultured calla explants. Scientia Horticulture 101, 305–313. El-Shamy M.A, El-Feky A.H. M, Eliwa N.Y.L. 2009. Propagation of calla lily (Zantedeschia aethiopica Spreng) plants by tissue culture technique. Bulletin of Faculty of Agriculture, Cairo University 60, 99-105. Habiba U, Reza S.H, Saha M.L, Rkhan M, Hadiuzzaman S. 2002. Endogenous bacterial contamination during in vitro culture of table banana: identification and Prevention- Plant Cell Tissue and Organ Culture 12, 117-124. Hazarika N, Teixeira da Silva J.A, Talukdar A. 2006. Effective Acclimatization of in Vitro Cultured Plants: Methods, Physiology and Genetics. Chapter in: Floriculture, Ornamental and Plant Biotechnology. Global Science Books, UK, Volume II. Hubner S. 2017. International statistics Flowers and Plants (Compilation). International association of Horticultural Producers (AIPH). www.aiph.org/statistical-yearbook. 65: ISSN 2313-7126. Janick J. 2007. Horticultural reviews. Purdue University. Published by John Wiley and Sons, Inc., Hoboken, New Jersey. 34: 455 p. Jao R.C, Lai C.C, Fang W, Chang F. 2005. Effects of red light on the growth of Zantedeschia plantlets in vitro and tuber formation using light-emitting diodes. Hortscience 40, 436-438. Kim K.S, De Hertogh A.A. 1997. Tissue culture of ornamental flowering bulbs (geophytes). Horticulture Reviews 18, 87-169. Koech A.A, Isutsa D.K, Wu Q. 2005. Explants, hormones and sucrose influence in vitro shoot regeneration and rooting of calla lily (Zantedeschia albomaculata L. Spreng.) ‘Black Magic’. J. Journal of Agriculture, Science and Technology 7, 53–66. Kozak D, Stelmaszczuk M. 2009. The effect of benzyladenine on shoot regeneration in vitro of Zantedeschia aethiopica ‘Green Goddess’. Annales UMCS, Horticultura 19, 14-18. Kritzinger EM, Vuuren RJV, Woodward B, Rong IH, Spreeth MH, Slabbert MM. 1998. Elimination of external and internal contaminants in rhizomes of Zantedeschia aethiopica with commercial fungicides and antibiotics. Plant Cell, Tissue and Organ Culture 52, 61-65. Kulpa D. 2016. Micropropagation of calla lily (Zantedeschia rehmannii). Folia Horticulture 28, 181-186. Langens-Gerrits M, Albers M, De Klerk G.J. 1997. Hot-water treatment before tissue culture reduces initial contamination in Lilium and Acer. Pathogen and microbial contamination management in micropropagation 12, 219-224. Małgorzata M, Bach A. 2014. Induction of bulb organogenesis in in vitro cultures of tarda tulip McCartan S.A, Beckett R.P, Ivanova M.V, Van Staden J. 2004. In vitro hardening- the role of ventilation on acclimation stress in Kniphofia leucocephala. Plant Growth Regulation 43, 49-55. Mojtahedi N, Koobaz P, Fathi M., Dabirashrafi O, Azadi P, Khosravi S. 2014. Maturating, Enlarging and Breaking Dormancy of In Vitro Lilium Bulblets. International Journal of Horticultural Science and Technology 1(2), 101-109. Pospisilova J, Ticha I, Kadlecek P, Haisel D, Plzakova S. 1999. Acclimatization of micropropagated plants to ex vitro conditions. Biologia Plantarum 42(4), 481-497. Sah S. K, Kaur A, Sandhu J.S. 2014. High Frequency Embryogenic Callus Induction and Whole Plant Regeneration in Japonica Rice Cv. Kitaake. Journal of Rice Research and Developments 2, 125. Santos A, Fernanda F, Irma M, Moraes S, Salema R. 2002. In vitro bulb formation of Narcissus asturiensis, a threatened species of the Amaryllidaceae. Journal of Horticultural Science and Biotechnology 77, 149-152. Sinha P, Roy S. K. 2002. Plant Regeneration through In vitro Cormel Formation from Callus Culture of Gladiolus primulinus Baker, Plant Tissue Culture 12, 139-145. Wei Z, Zhang H, Wang Y, Li Y, Xiong M, Wang X, Zhou D. 2017. Assessing Genetic Diversity and Population Differentiation of Colored Calla Lily (Zantedeschia Hybrid) for an Efficient Breeding Program. Genes 8, 168. Yu W.C, Joyce P.J, Cameron D.C, McCown B.H. 2000. Sucrose utilization during potato microtuber growth in bioreactors. Plant Cell Reports 19, 407-413. Zhang X, Wu Q, Li X, Zheng S, Wang S, Guo L, Zhang L, Custers J. 2011. Haploid plant production in Zantedeschia aethiopica ‘Hong Gan’ using anther culture. Scientia Horticulturae 129, 335-342. | ||
|
آمار تعداد مشاهده مقاله: 750 تعداد دریافت فایل اصل مقاله: 951 |
||