پیوندهای مفید
اخبار و اعلانات
آمار
| تعداد نشریات | 158 |
| تعداد شماره‌ها | 6,230 |
| تعداد مقالات | 67,764 |
| تعداد مشاهده مقاله | 115,193,072 |
| تعداد دریافت فایل اصل مقاله | 89,941,242 |
ارزیابی اثرآنتاگونیستی چند جدایه از باکتری‌های جنس Bacillus بر کنترل بیماری پوسیدگی طوقه و ریشه گندم با عامل Fusarium pseudograminearum | ||
| کنترل بیولوژیک آفات و بیماری های گیاهی | ||
| دوره 9، شماره 2، بهمن 1399، صفحه 115-126 اصل مقاله (631.14 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22059/jbioc.2021.326484.307 | ||
| نویسندگان | ||
| محسن ساسانی1؛ مسعود احمدزاده* 2 | ||
| 1گروه گیاه پزشکی، دانشکده کشاورزی، پردیس کشاورزی و منابع طبیعی، دانشگاه تهران | ||
| 2گیاه پزشکی | ||
| چکیده | ||
| گندم یکی از مهمترین محصولات کشاورزی در ایران است که نقش مهمی درتامین امنیت غذایی کشور دارد، بیماری پوسیدگی طوقه و ریشه گندم با عامل F. pseudograminearum ، از مهمترین بیمارگرهای گندم است که هر ساله موجب ایجاد خسارت‌های اقتصادی می‌شود، در این مطالعه 80 جدایه باکتری جنس باسیلوس از ریزوسفر مزارع گندم شهرستان‌های استان همدان؛ جداسازی شد، در آزمون کشت متقابل به ترتیب جدایه‌های UTB 3 و UTB 11 و UTB 22 و UTB 4 و UTB 7 با 60، 55، 37.5، 22.5 و 20 درصد بیشترین توانایی را در ممانعت از رشد قارچ F. pseudograminearum نشان دادند، در آزمون تاثیر متابولیت‌های فرّار نیز UTB 3 و UTB 7 و UTB 11 به‌ترتیب با 54، 52 و 49 درصد بیشترین درصد بازداری از رشد قارچ را نشان دادند. این جدایه‌ها همچنین توانایی تثبیت نیتروژن، تولید سیدروفور و آنزیم‌های خارج سلولی موثر در کنترل بیولوژیک (پروتئاز، لیپاز و کیتیناز) را داشتند، اما هیچکدام از جدایه‌ها قادر به تولیدآنزیم فسفاتاز نبودند. بررسی‌های گلخانه‌ای که به‌صورت تیمار بذور گندم با جدایه-های برتر انجام شد نشان داد که جدایه UTB 3 توانست بیماری پوسیدگی طوقه و ریشه گندم را به میزان 80.33 درصد در مقایسه با تیمار شاهد کنترل کند، همچنین این جدایه توانست موجب افزایش شاخص‌های رشدی گندم (طول ساقه و وزن خشک ریشه)، در حضور و عدم حضور قارچ بیمارگر گردد. لذا این جدایه به عنوان جدایه برتر جهت کنترل بیماری پوسیدگی طوقه و ریشه و افزایش شاخص‌های رشدی گندم معرفی می‌گردد. | ||
| کلیدواژه‌ها | ||
| باکتری؛ کنترل بیماری؛ تحریک رشد | ||
| عنوان مقاله [English] | ||
| Evaluation of antagonistic effect of several strain of Bacillus bacteria on control of crown and root rot of wheat with Fusarium pseudograminearum | ||
| نویسندگان [English] | ||
| mohsen sasani1؛ Masud Ahmadzadeh2 | ||
| 1Department of Plant Protection, Faculty of Agriculture, Campus of Agriculture and Natural Resources, University of Tehran | ||
| 2plant protection | ||
| چکیده [English] | ||
| Wheat is one of the most important agricultural products in Iran, that plays an important role in ensuring food security. Crown and root rot disease by F. pseudograminearum is one of the most important pathogens that cause economic damages every year. In this study, 80 isolates of Bacillus were isolated from wheat rhizosphere. In dual-culture test, UTB 3, UTB 11, UTB 22, UTB 4 and UTB 7 isolates with 60, 55, 37.5, 22.5 and 20% maximum ability, respectively. In effect test of volatile metabolites on inhibination the growth of F. pseudograminearum, UTB 3, UTB 7 and UTB 11 with 54, 52 and 49% showed the highest percentage of inhibition of fungal growth. These isolates were also capable of nitrogen fixation, production of siderophore and extracellular enzymes involved in biological control (protease, lipase and chitinase), but none of the isolates were able to produce phosphatase enzyme. Greenhouse studies by treating wheat seeds with selected isolates showed that UTB 3 was able to control crown and root rot of wheat by 80.33% compared to the control treatment. Also, this isolate could increase the growth indices of wheat (stem length and root dry weight) in the presence and absence of pathogenic fungi. Therefore, this isolate is introduced as the selected isolate to control crown and root rot disease and increase wheat growth indices. | ||
| کلیدواژه‌ها [English] | ||
| Bacteria, Disease control, Growth promotion | ||
| مراجع | ||
|
Ahmadzadeh M and Sharifi Tehrani (2021) Plant probiotic bacteria. University of Tehran Press, 629pp.
Alexander DB, Zuberer DA (1991) Use of chrome azurol S reagent evaluates siderophore production by rhizosphere bacteria. Biology and Fertility of Soils 12: 39-45.
Bajaj BK, Sharma P (2011) An alkali-thermo tolerant extracellular protease from a newly isolated Streptomyces sp. DP2. New Biotechnology 28(6): 725-732.
Buzzini P, Martini A (2002) Extracellular enzymatic activity profiles in yeast and yeast‐like strains isolated from tropical environments. Journal of Applied Microbiology 93(6): 1020-1025.
Cao Y, Zhang, Z, Ling, N, Yuan, Y, Zheng, X, Shen, B. and Shen, Q (2011) Bacillus subtilis SQR 9 can control Fusarium wilt in cucumber by colonizing plant roots. Biology and Fertility of Soils 47(5): 495-506.
Cook RJ (2000) Advances in plant health management in the twentieth century. Annual Review of Phytopathology, 38(1): 95-116.
FAO (2018) FAOSTAT Database on Agriculuyre, Food and Agiculture Organization of the United Nation.
Fiddaman PJ, Rossall S (1994) Effect of substrate on the production of antifungal volatiles from Bacillus subtilis. Journal of Applied Bacteriology 76(4): 395-405.
Ghazy N, El-Nahrawy S (2021) Siderophore production by Bacillus subtilis MF497446 and Pseudomonas koreensis MG209738 and their efficacy in controlling Cephalosporium maydis in maize plant. Archives of Microbiology 203(3): 1195-1209.
Glick WH, Miller CC, Cardinal LB (2007) Making a life in the field of organization science. Journal of Organizational Behavior 28(7): 817-835.
Haas D, Keel C (2003) Regulation of antibiotic production in root-colonizing Pseudomonas spp. and relevance for biological control of plant disease. Annual Review of Phytopathology, 41(1): 117-153.
Hashem A, Tabassum B, Abd_Allah EF (2019) Bacillus subtilis: A plant-growth promoting rhizobacterium that also impacts biotic stress. Saudi Journal of Biological Sciences, 26(6): 1291-1297.
Júnior AG, dos Santos ÁF, Auer CG (2000) Perspectivas do uso do controle biológico contra doenças florestais. Floresta, 30(1-2): 155-166. Kheirandish Z, Harighi B (2015) Evaluation of bacterial antagonists of Ralstonia solanacearum, causal agent of bacterial wilt of potato. Biological Control 86: 14-19.
Ku, Y, Xu, G, Tian, X, Xie, H, Yang, X. and Cao, C (2018) Root colonization and growth promotion of soybean, wheat and Chinese cabbage by Bacillus cereus YL6. PLoS One 13(11), p.e0200181.
Ling N, Xue C, Huang Q, Yang X, Xu Y, Shen Q (2010) Development of a mode of application of bioorganic fertilizer for improving the biocontrol efficacy to Fusarium wilt. Biocontrol 55(5): 673- 683. Mansoori B, Pazhomand MA (2005) Field reactions of some wheat advance lines and cultivars to common root and crown rot pathogens in Fars province. Seed and Plant 21(1): 81-91.
Miladiarsi NRM, Widyastuti R (2017) Selection, Characterization and Application of Rhizobacteria and its Effect on Chili (Capsicum annuum L.) Plant Growth. Research Journal of Microbiology 12: 161-169.
Mueller DS, Nelson RL, Hartman GL, Pedersen WL (2003) Response of commercially developed soybean cultivars and the ancestral soybean lines to Fusarium solani f. sp. glycines. Plant Disease 87(7): 827-831.
Obanor F, Neate S, Simpfendorfer S, Sabburg R, Wilson P, Chakraborty S (2013) Fusarium graminearum and Fusarium pseudograminearum caused the 2010 head blight epidemics in Australia. Plant Pathology 62(1): 79-91.
Saechow S, Thammasittirong A, Kittakoop P, Prachya S, Thammasittirong SNR (2018) Antagonistic activity against dirty panicle rice fungal pathogens and plant growth-promoting activity of Bacillus amyloliquefaciens BAS23. Journal of Microbiology and Biotechnology 28(9): 1527-1535.
Safaei D, Younesi H, Shaykh al-Islami M (2012) Fusarium species causing root and crown rot of wheat in Kermanshah province. Journal of Plant Diseases 48(2): 265-268.
Sasani M, Ahmadzadeh M (1399) Seed bioprim with Bacillus velezensis UTB96 to control the fungal pathogen of root and crown rot (Fusarium psudograminearum) and to improve some growth indices of wheat. Iranian Journal of Seed Science and Technology.
Shoda M (2000) Bacterial control of plant diseases. Journal of Bioscience and Bioengineering 89(6): 515-521.
Smiley RW (2009) Water and temperature parameters associated with winter wheat diseases caused by soilborne pathogens. Plant Disease 93(1): 73-80.
Smiley RW, Yan H (2009) Variability of Fusarium crown rot tolerances among cultivars and lines of spring and winter wheat. Plant Disease 93: 945-961.
Sperber JI (1958) The incidence of apatite-solubilizing organisms in the rhizosphere and soil. Australian Journal of Agricultural Research 9(6): 778-781. Thammasittirong SNR (2016) In vitro Inhibitory effect of Bacillus subtilis BAS114 against Curvularia lunata. Advances in Environmental Biology 10(1): 176-183.
Wagacha JM, Muthomi JW (2007) Fusarium culmorum: Infection process, mechanisms of mycotoxin production and their role in pathogenesis in wheat. Crop Protection 26: 877-885.
Weisburg WG, Barns SM, Pelletier DA, Lane DJ (1991) 16S ribosomal DNA amplification for phylogenetic study. Journal of Bacteriology 173(2): 697-703.
Yazdanparast R (1993) Screening for starch-hydrolysing bacteria. Medical Journal of The Islamic Republic of Iran (MJIRI) 7(1): 35-41.
Yousuf J, Thajudeen J, Rahiman M, Krishnankutty SP, Alikunj A, Abdulla MH (2017) Nitrogen fixing potential of various heterotrophic Bacillus strains from a tropical estuary and adjacent coastal regions. Journal of Basic Microbiology 57(11): 922-932. | ||
|
آمار تعداد مشاهده مقاله: 485 تعداد دریافت فایل اصل مقاله: 471 |
||