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بررسی تأثیر پوتریسین و 24- اپیبراسینولید بر فعالیت آنزیمهای ضداکسنده گیاه ریحان تحت شرایط تنش خشکی | ||
به زراعی کشاورزی | ||
مقاله 21، دوره 24، شماره 2، تیر 1401، صفحه 563-582 اصل مقاله (1.34 M) | ||
نوع مقاله: مقاله پژوهشی | ||
شناسه دیجیتال (DOI): 10.22059/jci.2022.314936.2487 | ||
نویسندگان | ||
فرشته دارابی1؛ نصرت اله عباسی* 2؛ محمدجواد زارع3 | ||
1دکتری فیزیولوژی گیاهان زراعی، گروه زراعت و اصلاح نباتات، دانشکده کشاورزی، دانشگاه ایلام، ایلام، ایران. | ||
2استادیار، گروه زراعت و اصلاح نباتات، دانشکده کشاورزی، دانشگاه ایلام، ایلام، ایران. | ||
3دانشیار، گروه زراعت و اصلاح نباتات، دانشکده کشاورزی دانشگاه ایلام، ایلام، ایران. | ||
چکیده | ||
هدف آزمایش بررسی و ارزیابی تأثیر پوتریسین و 24- اپیبراسینولید در تغییر فعالیت آنزیمهای ضداکسنده جهت تحمل به تنش خشکی در گیاه ریحان بود. طرح بهصورت اسپلیتپلات فاکتوریل در قالب طرح پایه بلوکهای کامل تصادفی با سه تکرار در سالهای زراعی 97- 1396 و 98-1397 در مزرعه تحقیقاتی دانشکده کشاورزی دانشگاه ایلام اجرا شد. تیمارهای آزمایش شامل تیمار آبی در سه سطح (40، 80 و 120 میلیمتر تبخیر از تشتک تبخیر کلاس A) در کرتهای اصلی، محلولپاشی پوتریسین در سه سطح (صفر (شاهد)، 5/0 و 2 میلیمولار) و 24-اپیبراسینولید در سه سطح (صفر (شاهد)، 5/0 و 2 میکرومولار) بهصورت فاکتوریل در کرتهای فرعی اجرا شد. نتایج نشان داد که بیشترین میزان پرولین در تیمارآبی 120 میلیمتر تبخیر از تشتک تبخیر در سال اول آزمایش حاصل شد و کاربرد پوتریسین (دو میلیمولار) و 24-اپیبراسینولید (5/0 میکرومولار) نیز سبب افزایش بیشتر میزان پرولین گیاه ریحان شد. در تیمار120 میلیمتر تبخیر از تشتک تبخیر و کاربرد دو میلیمولار پوتریسین بیشترین میزان آنزیم کاتالاز مشاهده شد. برهمکنش اثرات تنش خشکی، پوتریسین و 24-اپی براسینولید نیز نشان داد که بیشترین میزان آنزیم آسکوربات پراکسیداز و سوپراکسید دیسموتاز در تیمار 120 میلیمتر تبخیر از تشتک تبخیر و بالاترین غلظت پوتریسین (دو میلیمولار) و 24- اپیبراسینولید (دو میکرومولار) مشاهده شد. در شرایط آبیاری مطلوب (40 میلیمتر تبخیر از تشتک تبخیر)، کاربرد همزمان غلظتهای کمتر پوتریسین (5/0 میلیمولار) و اپی براسینولید (5/0 میکرومولار) و در شرایط تنش خشکی (80 و 120 میلیمتر تبخیر از تشتک تبخیر)، غلظت بیشتر این دو ماده (دو میلیمولار پوتریسین و دو میکرومولار اپیبراسینولید) باعث افزایش آنزیم گایاکول پراکسیداز شد. برهمکنش چندگانه سال، تنش خشکی، پوترسین و 24- اپیبراسینولید تأثیر معنیداری بر فلاونوِئید کل و درصد اسانس ریحان گذاشت بهنحوی که در هر دو سال آزمایش، کاربرد غلظت 2 میلیمولار پوترسین و 2 میکرومولار 24-اپیبراسینولید در همه سطوح آبی بهویژه 120 میلیمتر تبخیر از تشتک تبخیر، سبب افزایش بیشتر این دو صفت شد و در سال اول آزمایش این افزایش بیشتر از سال دوم بود. کاربرد همزمان دو میلیمولار پوترسین و دو میکرومولار اپیبراسینولید بهدلیل افزایش سنتز پرولین و آنزیمهای آنتیاکسیدانی بهترین تیمار در جهت کاهش اثرات تنش خشکی در گیاه ریحان بود، که نشاندهنده وجود رابطه همافزایی بین این دو ماده در بهبود رشد گیاه ریحان و افزایش درصد اسانس این گیاه است. | ||
کلیدواژهها | ||
اسانس؛ آنتی اکسیدان؛ پرولین؛ کاتالاز؛ گایاکول پراکسیداز | ||
عنوان مقاله [English] | ||
The Effect of Putrescine and 24-Epibrasinolide on the Activity of Antioxidant Enzymes in Basil to under Drought Stress | ||
نویسندگان [English] | ||
fereshteh darabi1؛ Nosratollah Abbasi2؛ Mohammad Javad Zarea3 | ||
1Ph.D. in Crop Physiology, Department of Agronomy and Plant Breeding, Faculty of Agriculture, Ilam University, Ilam, Iran. | ||
2Assistant Professor, Department of Agronomy and Plant Breeding, Faculty of Agriculture, Ilam University, Ilam, Iran. | ||
3Associate Professor, Department of Agronomy and Plant Breeding, Faculty of Agriculture, Ilam University, Ilam, Iran. | ||
چکیده [English] | ||
This paper aims at evaluating the effects of putrescine and 24-epibrasinolide on altering the activity of antioxidant enzymes to tolerate drought stress in basil. The plan has been implemented as factorial split plot layout based on a randomized complete block design with three replications in the cropping years 2017-2018 and 2018-2019 at the research farm of the Faculty of Agriculture, Ilam University. Experimental treatments include drought stress at three levels (40, 80, and 120 mm evaporation from Class A evaporation pan) as the main plots, putrescine foliar application at three levels (0, 0.5, and 2 (mM) and 24- Epibrassinolid foliar application at three levels (0, 0.5, and 2 μM) as subplots. Results show that the highest amount of proline in 120 mm occurs during the first year of the experiment and the use of putricin (2 mM) and 24-epibrasinolide (0.5 μM) also cause a further increase in proline. The highest amount of catalase has been observed in the 120 mm evaporation treatment and the application of 2 mM putrescine. The interaction of drought stress, putrescine, and 24-epiprasinolide also show that the highest levels of ascorbate peroxidase and superoxide dismutase belong to the treatment of 120 mM where the highest concentrations of putrescine (2 mM) and 24-epibrasinolide (2 μM) is observed. Under optimal irrigation conditions (40 mm), the combined application of lower concentrations of putrescine (0.5 mM) and epibrasinolide (0.5 μM) and under drought stress conditions (80 and 120 mm), higher concentrations of these two substances (2 mM putrescine and 2 μM epibrasinolide) increase the enzyme guaiacol peroxidase. Multiple interactions of year, drought stress, putrescine, and 24-epibrasinolide have had a significant effect on total flavonoids and essential oil percentage of basil. In both experimental years, application of 2 mM putrescine and 2 μM 24-epibrasinolide at all levels of drought stress 120 mm cause a further increase in these two traits which has been greater in the first year than the second one. Concomitant use of 2 mM putrescine and 2 μM epibracinolide due to increased proline synthesis and antioxidant enzymes is the best treatment to reduce the effects of drought stress in basil, indicating a synergistic relation between the two. The substance boosts the growth of basil, increasing the percentage of its essence. | ||
کلیدواژهها [English] | ||
Antioxidants, Catalase, Essence, Guaiacol peroxidase, Essential oil, Proline | ||
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