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شناسایی مولکولی قارچ های راستۀ موکورال در مناطق آلوده به موادنفتی | ||
نشریه محیط زیست طبیعی | ||
مقاله 6، دوره 74، شماره 3، آذر 1400، صفحه 490-504 اصل مقاله (943.75 K) | ||
نوع مقاله: مقاله پژوهشی | ||
شناسه دیجیتال (DOI): 10.22059/jne.2021.318171.2161 | ||
نویسندگان | ||
سمیه دولت آبادی* 1؛ محمد جواد نجف زاده2؛ نعمت الله جعفرزاده حقیقی فرد3 | ||
1استادیار گروه زیست شناسی، دانشکده علوم، دانشگاه حکیم سبزواری، سبزوار، ایران | ||
2استاد گروه انگل شناسی و قارچ شناسی، دانشکده پزشکی، دانشگاه علوم پزشکی مشهد، مشهد، ایران | ||
3مرکز تحقیقات فناوریهای محیط زیست، دانشگاه علوم پزشکی جندی شاپور اهواز، اهواز، ایران | ||
چکیده | ||
موکورالها گروهی از قارچهای اولیه از خانوادۀ زیگومیستها هستند که در محیطهای مختلف در سراسر جهان دیده میشوند. این قارچها در تولید مواد غذایی تخمیرشده، متابولیتهای ثانویه و آنزیمها و همچنین عفونت قارچی موکورمایکوزیس مؤثرند. با توجه به رشد سریع این قارچها، حضور آنها در اکوسیستمهای مختلف و توانایی آنها در تولید آنزیمها در پی شناخت اثر این قارچها در فرایندهای زیستپالایی محیطهای آلوده به مواد نفتی هستیم. در این پژوهش جداسازی نمونهها به روش شناورسازی در روغن از نمونههای آلوده به مواد نفتی انجام گرفت و شناسایی آنها براساس مشخصههای مورفولوژیکی و مولکولی از طریق توالی یابی ناحیۀ ژنی ITS-rDNA و ترسیم درخت فیلوژنتیکی صورت پذیرفت. در مجموع 131 جدایۀ قارچی استحصال شد که از این میان 27 نمونه متعلق به راستۀ موکورالها بود. این جدایههای بومی ایران با استفاده از روشهای مورفولوژیکی و مولکولی بهطور دقیق و در حد گونه شناسایی شد و سپس در کلکسیونهای قارچی هلند(Westerdijk Institute) و لهستان (Warsaw University) ذخیره و شمارۀ مرجع کلکسیون برای آنها دریافت شد. جدایههای قارچی بهدستآمده شامل گونههای Rhizopus (n=19), Mucor (n=4), Actinomucor (n=2), Lichtheimia (n=1), Cunninghumella (n=1) بود که با توجه به قابلیت بالقوۀ رشد آنها در محیطهای آلوده به مواد نفتی از توان زیستپالایی برخوردارند. | ||
کلیدواژهها | ||
آلودگی نفتی؛ شناسایی مولکولی؛ مورفولوژی؛ موکورالها | ||
عنوان مقاله [English] | ||
Molecular identification of mucoralean fungi isolated from the oil-polluted sites | ||
نویسندگان [English] | ||
Somayeh Dolatabadi1؛ Muhammed javad Najafzadeh2؛ Neematollah Jaafarzadeh3 | ||
1Assistant Professor, Department of Biology, Faculty of Science, Hakim Sabzevari University, Sabzevar, Iran | ||
2Professor, Department of Parasitology, Mashhad University of Medical Science, Mashhad, Iran | ||
3Ahvaz Jondishapur University of Medical Sciences | ||
چکیده [English] | ||
Mucoralean fungi are a group of basal fungi related to zygomycetes which can be found in different environments all over the world. They are a part of fermented food production, secondary metabolites and enzymes. Considering their fast growth and their ability to produce a different sort of enzymes, we seek for their role in oil-polluted sites’ bioremediation. Using oil floating method, fungal isolates were collected and identified both morphologically and molecularly using ITS gene as a barcoding marker and phylogenetic tree. In total, 131 isolates were collected. Among them, 27 mucoralean fungi were listed as follows: Rhizopus (n=19), Mucor (n=4), Actinomucor (n=2), Lichtheimia, and Cunninghamella one isolate per each. These isolated strains from Iran were identified correctly based on molecular methods and then deposited in the culture collections of the Netherlands (Westerdijk Institute) and Warsaw University. Being isolated from oil-polluted environments, these isolates have bioremediation potentials. | ||
کلیدواژهها [English] | ||
Oil pollution, Molecular identification, Morphology, Mucorales | ||
مراجع | ||
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