Extraction of phycocyanin from Arthrospira platensis with different solvents and methods | ||
| شیلات | ||
| Volume 75, Issue 2 - Serial Number 4, June 2022, Pages 183-192 PDF (987.86 K) | ||
| Document Type: Research Paper | ||
| DOI: 10.22059/jfisheries.2021.333458.1292 | ||
| Authors | ||
| Fahimeh Shahhosseini1; Mohammad Ali Nematollahi* 2; Seyed Vali Hosseini3 | ||
| 1MSc Graduated, Department of Fisheries, Faculty of Natural Resources, University of Tehran, Karaj, Iran | ||
| 2Professor, Department of Fisheries, Faculty of Natural Resources, University of Tehran, Karaj, Iran | ||
| 3Associate Deprtment of Fisheries, Faculty of Natural Resources, University of Tehran | ||
| Abstract | ||
| In this study, phycocyanin pigment extraction from Arthrospira platensis microalgae was carried out using various solvents (distilled water, phosphate buffered saline) and methods (sonication, microwave and freezing & thawing) for comparison and determine the best solvent and method in order to achieve higher extraction yield. Rate of 0.1 g A. platensis powder in 10 cc of solvent (distilled water and phosphate buffered saline) was similar in each three extraction method whereas the conditions of extraction methods sonication, microwave and freezing & thawing as follows, respectively: 50% amplitude and 15 min, 100 W and 3 min, frozen at -20 ± 2 °C for 24 h and thawing at room temperature. The analysis of variance was obtained for data mean ± SD (standard deviation) using SPSS in confidence level of (P < 0.05). Most extraction yield was obtained by phosphate buffered saline (PBS) and method Freezing & Thawing (pc= 146/3 ± 1/55 mg/g) and least by distilled water and method Microwave (pc= 94/4 ± 2/68 mg/g). . | ||
| Keywords | ||
| Arthrospira platensis; Phycocyanin; Extraction; Sonication; Microwave | ||
| References | ||
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