Biomarker Responses in Sclerophrys regularis (Anura: Bufonidae) Exposed to Atrazine and Nitrate

Abdel-Wahab, M., Yassien, M. H., Thabet, A, A., Said, R.E.M., Mahdy, A., Amer. O.S.O. and Saber, S. A. (2022). Seasonal variations of some heavy metal concentrations in seawater, sediment, and the surf clam, Mactra olorina (Philippi, 1846) in the Great Bitter Lake, Suez Canal, Egypt. Egyptian Journal of Aquatic Biology and Fisheries 26(1), 83-98.
Ahmad, K. and Saleh, K. (2010). Clastogenic studies on Tandaha Dam water in Asser.  16, 33-42.
Akhtar, N., Fiaz Khan, M., Tabassum, S. and Zahran, E. (2021) Adverse effects of atrazine on blood parameters, biochemical profile and genotoxicity of snow trout (Schizothorax plagiostomus). Saudi journal of biological sciences 28(3), 1999-2003.
Allran, J. W. and Karasov, W. H. (2000). Effects of atrazine and nitrate on northern leopard frog (Rana pipiens) larvae exposed in the laboratory from posthatch through metamorphosis. Environmental Toxicology and Chemistry 19, 2850-2855.
AmphibiaWeb (2020). Worldwide Amphibian Declines: What is the scope of the problem, what are the causes, and what can be done?
Baker, N.J., Bancroft, B.A. and Garcia, T.S. (2013). A meta-analysis of the effects of pesticides and fertilizers on survival and growth of amphibians. Science of The Total Environment 449, 150-156.
Becker, C.G., Fonseca, C.R., Haddad, C.F.B., Batista, R.F. and Prado, P.I. (2007). Habitat split and the global decline of amphibians. Science 318(5857), 1775-1777.
Bionda, C., Salas, N., Caraffa, E., Baraquet, M. and Martino, A. (2012). On abnormalities recorded in an urban population of Rhinella arenarum from central Argentina.
Brühl, C.A., Schmidt, T., Pieper, S. and Alscher, A. (2013). Terrestrial pesticide exposure of amphibians: An underestimated cause of global decline? Scientific Reports 3(1), 1135
Brooks, S.J., Harman, C., Hultman, M.T. and Berge, J.A. (2015). Integrated biomarker assessment of the effects of tailing discharges from an iron ore mine using blue mussels (Mytilus spp.). Science of The Total Environment 524-525, 104-114.
Cardwell, R. D., Purdy, R. and Bahner, R. C. (2011). Aquatic toxicology and hazard assessment : Seventh symposium.
Chang, J., Liang, C., Wang, Y., Yong, L., Mao, W., Yang, H., Jia, X., Liu, Z. and Song, Y. (2021). Toxic effects of atrazine on immune function in BALB/c mice. Environmental Science and Pollution Research 28, 1-17.
Cruz-Santiago, O., Castillo, C.G., Espinosa-Reyes, G., Pérez-Maldonado, I.N., González-Mille, D.J., Cuevas-Díaz, M.d.C. and Ilizaliturri-Hernández, C.A. (2022). Giant Toads (Rhinella marina) From the Industrial Zones of Low Basin of the Coatzacoalcos River (Veracruz, MX) Presents Genotoxicity in Erythrocytes. Bulletin of Environmental Contamination and Toxicology 108(1), 64-70.
Davidson, C. (2004). Declining downwind: amphibian population declines in California and historical pesticide use. Ecological Applications 14(6), 1892-1902.
Davidson, C. and Knapp, R.A. (2007). Multiple stressors and amphibian declines: dual impacts of pesticides and fish on yellow‐legged frogs. Ecological Applications 17(2), 587-597.
Earl, J. and Whiteman, H. (2009). Effects of pulsed nitrate exposure on amphibian development. Environmental toxicology and chemistry / SETAC 28, 1331-1337.
Edwards, T.M., McCoy, K.A., Barbeau, T., McCoy, M.W., Thro, J.M. and Guillette, L.J. (2006). Environmental context determines nitrate toxicity in Southern toad (Bufo terrestris) tadpoles. Aquatic Toxicology 78(1), 50-58.
Egea-Serrano, A., Tejedo, M. and Torralva, M. (2011). Behavioral responses of the Iberian waterfrog, Pelophylax perezi (Seoane, 1885), to three nitrogenous compounds in laboratory conditions. Ecotoxicology Ecotoxicology 20, 1246-1257.
Ehrsam, M., Knutie, S.A. and Rohr, J.R. (2016). The herbicide atrazine induces hyperactivity and compromises tadpole detection of predator chemical cues. Environmental Toxicology and Chemistry 35(9), 2239-2244.
Ezemonye, L. I. N. and Tongo, I. (2009). Lethal and sublethal effects of atrazine to amphibian larvae. Jordan Journal of Biological Sciences : an International Peer-reviewed Scientific Journal. 2, 29-36.
Farrag, M.M., Said, R.E., Elmileegy, I.M., Abou Khalil, N.S., El-Sawy, M.F. and Osman, A.G. (2022). Effects of penconazole and copper nanoparticle fungicides on redbelly tilapia, Coptodon zillii (Gervais, 1848): Reproductive outcomes. International Journal of Aquatic Biology 9(6), 370-382.
Feng, S., Kong, Z., Wang, X., Zhao, L. and Peng, P. (2004). Acute toxicity and genotoxicity of two novel pesticides on amphibian, Rana N. Hallowell. Chemosphere 56, 457-63.
Garaj-Vrhovac, V., Gajski, G. and Ravli , S. (2008). Efficacy of HUMN criteria for scoring the micronucleus assay in human lymphocytes exposed to a low concentration of p,p’-DDT. Brazilian Journal of Medical and Biological Research 41, 473-476.
Gosner, K. L. (1960). A Simplified table for staging anuran embryos andlarvae with notes on Identification. Herpetologica 16, 183-190.
Güner, U., Dilek, F. and Muranm1, G. (2011). Micronucleus Test, Nuclear abnormalities and accumulation of Cu and Cd on Gambusia affinis (Baird & Girard, 1853). Turkish Journal of Fisheries and Aquatic Sciences 11, 615-622.
Gürcü, B., Koca, S., Başimoğlu Koca, Y., Çöllü, F. and Tuğlu, M.I. (2022). Genotoxic Effect, Oxidative Stress and Cell Death due to Metronidazole Application in Gills and Liver Tissues of Rainbow Trout (Oncorhynchus mykiss). Pollution 8(3), 804-819.
Hamer, A.J., Makings, J.A., Lane, S.J. and Mahony, M.J. (2004). Amphibian decline and fertilizers used on agricultural land in south-eastern Australia. Agriculture, Ecosystems & Environment 102(3), 299-305.
Hayes, T. B., Khoury, V., Narayan, A., Nazir, M., Park, A., Brown, T., Adame, L., Chan, E., Buchholz, D., Stueve, T. and Gallipeau, S. (2010). Atrazine induces complete feminization and chemical castration in male African clawed frogs (Xenopus laevis). Proceedings of the National Academy of Sciences 107, 4612-4617.
Heckmann, J. G. (2009). Effects of atrazine, chlorpyrifos, and their interactions on anti-predator behavior and activity in African clawed frogs, Xenopus laevis.
Hecnar, S. J. (1995). Acute and chronic toxicity of ammonium nitrate fertilizer to amphibians from southern ontario. Environmental Toxicology and Chemistry 14, 2131-2137.
Huang, D., Zhang, Y., Wang, Y., Xie, Z. and Ji, W. (2007). Assessment of the genotoxicity in toad Bufo raddei exposed to petrochemical contaminants in Lanzhou Region, China. Mutation Research/Genetic Toxicology and Environmental Mutagenesis 629, 81-88.
Houlahan, J., Findlay, C.S., Schmidt, B.R., Meyer, A.H. and Kuzmin, S.L. (2000). Quantitative evidence for global amphibian population declines. Nature 404(6779), 752-755.
Ilha, P. and Schiesari, L. (2014). Lethal and Sublethal Effects of Inorganic Nitrogen on Gladiator Frog Tadpoles (Hypsiboas faber, Hylidae). Copeia 2014(2), 221-230.
Karlsson, A.S., Lesch, M., Weihermüller, L., Thiele, B., Disko, U., Hofmann, D., Vereecken, H. and Spielvogel, S. (2020). Pesticide contamination of the upper Elbe River and an adjacent floodplain area. Journal of Soils and Sediments 20(4), 2067-2081.
Krishnamurthy, S. (2006). Effects of nitrate on feeding and resting of tadpoles of Nyctibatrachus major (anura: Ranidae).
Mahdy, A., F. A. Ahmed, A., Y. M. Idris, M., M. A. Mohamed, I., A. A. Samie, M., A. M. Mayof, A., A. B. Emgower, S. and Said, R. E.M.  (2020). The effects of nesting ground temperatures on incubation and hatchability of loggerhead turtle Caretta caretta inhabiting the Mediterranean Sea Coast, Libya. Egyptian Journal of Aquatic Biology and Fisheries 24(5), 111-124.
Marco, A., Quilchano, C. and Blaustein, A. R. (1999). Sensitivity to nitrate and nitrite in pond-breeding amphibians from the Pacific Northwest, USA. Environmental Toxicology and Chemistry 18, 2836-2839.
Meteyer, C. U. (2000). “Field guide to malformations of frogs and toads: with radiographic interpretations,” Rep. No. 2000-0005, Reston, VA.
Nkontcheu, D.B.K., Tchamadeu, N.N., Ngealekeleoh, F. and Nchase, S. (2017). Ecotoxicological effects of imidacloprid and lambda-cyhalothrin (insecticide) on tadpoles of the African common toad, Amietophrynus regularis (Reuss, 1833)(Amphibia: Bufonidae). Emerging Science Journal 1(2), 49-53.
Ortiz-Santaliestra, M. E., Fernández-Benéitez, M. J., Marco, A. and Lizana, M. (2010). Influence of ammonium nitrate on larval anti-predatory responses of two amphibian species. Aquatic Toxicology 99, 198-204.
Ortiz-Santaliestra, M. E. and Marco, A. (2015). Influence of dissolved oxygen conditions on toxicity of ammonium nitrate to larval natterjack toads. Arch Environ Contam Toxicol 69, 95-103.
Osman, A. G. M., Abd El Reheem, A.-E.-B. M., Moustafa, M. A., Mahmoud, U. M., Abuel-Fadl, K. Y. and Kloas, W. (2011). In situ evaluation of the genotoxic potential of the river Nile: I. Micronucleus and nuclear lesion tests of erythrocytes of Oreochromis niloticus niloticus (Linnaeus, 1758) and Clarias gariepinus (Burchell, 1822). Toxicological & Environmental Chemistry 93, 1002-1017.
Ouellet, M. (2000). Amphibian deformities: current state of knowledge. Ecotoxicology of amphibians and reptiles, 617-661.
Paola, M., Peltzer, Rafael, C., LAJMANOVICH, Laura, Sánchez, Andrés, ATTADEMO, Celina, JUNGES, Clarisa, !=., BIONDA, Adolfo, Martino, and Bassó, A. (2011). Morphological abnormalities in amphibian populations from the mid-eastern region of Argentina.
Rajaguru, P., Kalpana, R., Hema, A., Suba, S., Baskarasethupathi, B., Kumar, P. and Kalaiselvi, K. (2001). Genotoxicity of some sulfur dyes on tadpoles (Rana hexadactyla) measured using the comet assay. Environmental and molecular mutagenesis 38, 316-322.
Richard , L. (2003). “Investigations into the causes of amphibian  malformations in the lake Champlain basin of New England “.
Rouse, J. D., Bishop, C. A. and Struger, J. (1999). Nitrogen pollution: an assessment of its threat to amphibian survival. Environ Health Perspect 107, 799-803.
Ryder, T. B., Parker, P. G., Blake, J. G. and Loiselle, B. A. (2009). It takes two to tango: reproductive skew and social correlates of male mating success in a lek-breeding bird. Proceedings. Biological sciences 276, 2377-2384.
Said, R. E. M. (2013). Using of some amphibians as bioindicator of aquatic pollution of Egyptian river Nile. PhD thesis, Al–Azhar university , Faculty of science, Assiut branch. Egypt, pp 284.
Smith, L. E., Parks, K. K., Hasegawa, L. S., Eastmond, D. A. and Grosovsky, A. J. (1998). Targeted breakage of paracentromeric heterochromatin induces chromosomal instability. Mutagenesis 13, 435-444.
Solomon, K. R., Baker, D. B., Richards, R. P., Dixon, K. R., Klaine, S. J., La Point, T. W., Kendall, R. J., Weisskopf, C. P., Giddings, J. M., Giesy, J. P., Hall Jr., L. W. and Williams, W. M. (1996). Ecological risk assessment of atrazine in North American surface waters. Environmental Toxicology and Chemistry 15, 31-76.
Stuart, S. N., Chanson, J. S., Cox, N. A., Young, B. E., Rodrigues, A. S., Fischman, D. L. and Waller, R. W. (2004). Status and trends of amphibian declines and extinctions worldwide. Science 306, 1783-6.
Sparling, D.W. and Fellers, G.M. (2009). Toxicity of two insecticides to California, USA, anurans and its relevance to declining amphibian populations. Environmental Toxicology and Chemistry: An International Journal 28(8), 1696-1703.
Sparling, D.W., Fellers, G.M. and McConnell, L.L. (2001). Pesticides and amphibian population declines in California, USA. Environmental Toxicology and Chemistry 20(7), 1591-1595.