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Ebrahimzadeh, Mohammad Hadi, Naseri, Mahboobeh, Younesikelaki, Fatemeh Soghra, Yusefi javan, Iman, Swamy N, Rama. (1402). Influence of Plant Growth Regulators on Callus Induction, Silymarin Production and Antioxidant Activity in Silybum marianum L. Gaertn. by Tissue Culture. , 10(2), 223-236. doi: 10.22059/ijhst.2022.322832.465
Mohammad Hadi Ebrahimzadeh; Mahboobeh Naseri; Fatemeh Soghra Younesikelaki; Iman Yusefi javan; Rama Swamy N. "Influence of Plant Growth Regulators on Callus Induction, Silymarin Production and Antioxidant Activity in Silybum marianum L. Gaertn. by Tissue Culture". , 10, 2, 1402, 223-236. doi: 10.22059/ijhst.2022.322832.465
Ebrahimzadeh, Mohammad Hadi, Naseri, Mahboobeh, Younesikelaki, Fatemeh Soghra, Yusefi javan, Iman, Swamy N, Rama. (1402). 'Influence of Plant Growth Regulators on Callus Induction, Silymarin Production and Antioxidant Activity in Silybum marianum L. Gaertn. by Tissue Culture', , 10(2), pp. 223-236. doi: 10.22059/ijhst.2022.322832.465
Ebrahimzadeh, Mohammad Hadi, Naseri, Mahboobeh, Younesikelaki, Fatemeh Soghra, Yusefi javan, Iman, Swamy N, Rama. Influence of Plant Growth Regulators on Callus Induction, Silymarin Production and Antioxidant Activity in Silybum marianum L. Gaertn. by Tissue Culture. , 1402; 10(2): 223-236. doi: 10.22059/ijhst.2022.322832.465

Influence of Plant Growth Regulators on Callus Induction, Silymarin Production and Antioxidant Activity in Silybum marianum L. Gaertn. by Tissue Culture

International Journal of Horticultural Science and Technology
دوره 10، شماره 2، تیر 2023، صفحه 223-236    اصل مقاله (1.33 M)
نوع مقاله: Research paper
شناسه دیجیتال (DOI): 10.22059/ijhst.2022.322832.465
نویسندگان
Mohammad Hadi Ebrahimzadeh1؛ Mahboobeh Naseri* 2؛ Fatemeh Soghra Younesikelaki3؛ Iman Yusefi javan4؛ Rama Swamy N5
1Department of Plant Production, Faculty of Agriculture, University of Torbat Heydarieh, Torbat Heydarieh, Iran
2Plant Production of University of Torbate Heydarieh, Iran
3Department of Biology, Payame Noor University, Iran
4University of Torbat heydarieh
5DOS in Biotechnology, Kakatiya University, Telangana, India
چکیده
Silybum marianum (L.) is a medicinally important herb known for producing the liver-protecting compound silymarin, an isomeric mixture of flavonolignans and taxifolin. In this study, a protocol (plant growth regulators in various concentrations and combinations of coconut water) for the initiation of callus and silymarin production using different types of explantes (root, hypocotyls, cotyledonary leaf) of S. marianum L. was established. The free radical scavenging activity of silymarin extracted from callus cultures of these explants was investigated. In comparison to other plant growth regulators and explants studied, root explants cultured on MS medium enriched with 5.0 M NAA + 2.5 M BAP + 10% coconut water produced the most friable callus and had the highest possible level of silymarin and free radical scavenging activity. NAA + BAP + coconut water was found to be the best hormonal combination for callus initiation and mass production. This research will aid in understanding the influence of plant growth regulators and different types of explantes on the production of valuable secondary metabolites in S. marianum L. in vitro cultures.
کلیدواژه‌ها
Antioxidant activity؛ Callus؛ Coconut water؛ Silymarin
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تعداد مشاهده مقاله: 519
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