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تاثیر حشره‌‌کش دیازینون بر بیان ژن‌‌های تنظیم کننده‌‌ی استرویید سازی و متیلاسیون DNA جایگاه پروموتر ژن StAR طی تکامل برون تنی اووسایت بز | ||
| علوم دامی ایران | ||
| دوره 55، شماره 1، فروردین 1403، صفحه 131-142 اصل مقاله (1.29 M) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22059/ijas.2022.345718.653897 | ||
| نویسندگان | ||
| منصوره قربانعلی نیا1؛ حمید دلدار* 2؛ قدرت رحیمی میانجی1؛ عیسی دیرنده1 | ||
| 1گروه علوم دامی دانشگاه علوم کشاورزی و منابع طبیعی ساری، ساری ، ایران | ||
| 2گروه علوم دامی ، دانشکده کشاورزی، دانشگاه گیلان، رشت ، ایران | ||
| چکیده | ||
| پژوهش حاضر به منظور بررسی تاثیر غلظت های متفاوت حشره‌‌کش دیازینون به عنوان یک سم پرکاربرد کشاورزی، بر بیان ژن‌‌های تنظیم کننده‌‌ی استرویید‌‌سازی و متیلاسیون جایگاه پروموتر ژن StAR در تکامل برون‌‌تنی اووسایت بز انجام شد. تخمدان‌‌های بز بلافاصله بعد از کشتار، از لاشه‌‌ی دام جدا و درون فلاسک حاوی سرم فیزیولوژیک به آزمایشگاه منتقل شدند. مجموعه اووسایت-کومولوس با روش برشی از فولیکول‌‌های آنترال کوچک جدا شدند و در محیط Medium 199 به همراه غلظت‌‌های متفاوت حشره‌‌کش دیازینون کشت داده شدند. پژوهش در قالب طرح کاملا تصادفی، با 5 تیمار آزمایشی شامل تیمارهای شاهد، 75 ، 5/37 و 75/18 میکرومولار دیازینون و تیمار دارای نیم میکرومولار DMSO، و در 4 تکرار انجام شد. نتایج پژوهش نشان داد غلظت‌‌های متفاوت دیازینون به طور معنی‌‌داری نرخ تکامل برون‌‌تنی اووسایت‌‌ها را نسبت به تیمار شاهد کاهش داد (05/0 >(P . هم‌‌چنین با افزودن غلظت‌‌های متفاوت دیازینون به محیط تکامل برون‌‌تنی اووسایت بز، بیان ژن‌‌های CYP11A1، 17βHSD، CYP19 در سلول‌‌های کومولوس کاهش نشان داد. اما در مورد ژن StAR افزایش بیان ژن مشاهده شد، این افزایش بیان ژن می‌‌تواند به علت کاهش میزان متیلاسیون DNA مشاهده شده در غلظت 75/18 میکرومولار از دیازینون باشد. چنین به نظر می‌‌رسد که حشره‌‌کش دیازینون اثر برهم زنندگی در نرخ تکامل برون‌‌تنی اووسایت و بیان ژن‌‌های استرویید سازی در سلول‌‌های کومولوس را دارد. | ||
| کلیدواژه‌ها | ||
| تکامل برون تنی اووسایت؛ حشره‌‌کش دیازینون؛ کمپلکس اووسایت کومولوس؛ متیلاسیون DNA | ||
| عنوان مقاله [English] | ||
| Effect of insecticide Diazinon on expression of steroidogenic genes and DNA methylation of promoter site of StAR gene during in vitro maturation of goat oocyte | ||
| نویسندگان [English] | ||
| Mansoureh Ghorbanalinia1؛ Hamid Deldar2؛ Ghodrat Rahimi Miyanji1؛ Essa Dirandeh1 | ||
| 1Department of Animal Science, Sari Agricultural and Natural Resources University, Sari, Iran | ||
| 2Department of Animal Science, Faculty of Agriculture, University of Guilan, Rasht, Iran. | ||
| چکیده [English] | ||
| This research was conducted to investigate the effect of different concentrations of Diazinon as a pesticide, that is widely used in agriculture, on the expression of steroidogenic genes and DNA methylation of promoter site of StAR gene in in vitro maturation of goat oocytes. To prepare goat oocytes, ovaries were prepared from a slaughterhouse and transported to the laboratory into the flask containing warm saline (30-34oC). Oocyte-cumulus complexes (COC) was removed from small antral follicles with the slicing method, and transferred to Medium 199. COCs were placed in a maturation medium for 24 hours, and were reached to metaphase stage Π (nuclear maturation). The experiment was examined in a completely randomized design with five treatments included: control, 75, 37.5, and 18.75 μM of Diazinon, and 0.5 μM of DMSO. The results showed that the addition of different concentrations of Diazinon in the goat maturation medium, significantly decreased the maturation rate (P <0.05) compared to the control treatment, and also it led to a decline in the gene expression of CYP11A1, 17βHSD, CYP19 in cumulus cells. However, the expression of StAR gene was increased. Therefore, it can be concluded that Diazinon has a disruptor effect on oocyte maturation rate, and expression of steroidogenic genes in cumulus cells. | ||
| کلیدواژه‌ها [English] | ||
| Insecticide Diazinon, in vitro maturation of oocyte, Oocyte cumulus complexes, DNA methylation | ||
| مراجع | ||
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