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بررسی انتقالپذیری ویروس رگبرگ روشنی زرد مرکبات (Citrus yellow vein clearing virus) توسط سه گونه شته غالب مرکبات | ||
| دانش گیاهپزشکی ایران | ||
| دوره 54، شماره 1، شهریور 1402، صفحه 101-115 اصل مقاله (1.42 M) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22059/ijpps.2023.353042.1007016 | ||
| نویسندگان | ||
| رضا مقصودی1؛ سعید نصرالله نژاد1؛ سیروس آقاجانزاده* 2؛ سید مهدی بنی هاشمیان2 | ||
| 1گروه گیاهپزشکی، دانشگاه علوم کشاورزی و منابع طبیعی گلستان، گرگان، ایران | ||
| 2پژوهشکده مرکبات و میوه‌های نیمه‌گرمسیری، موسسه تحقیقات علوم باغبانی، سازمان تحقیقات، آموزش و ترویج کشاورزی، رامسر، ایران. | ||
| چکیده | ||
| ویروس رگبرگ روشنی زرد مرکبات از جنس Mandarivirus است که بعضی از ارقام مرکبات را آلوده کرده و موجب خسارت می‌شود. در این مطالعه، انتقال این ویروس توسط شته سبز مرکبات (spiraecola Aphis)، شته جالیز (A. gossypii) و شته سیاه مرکبات (Toxoptera aurantii) مورد بررسی قرار گرفت. تعداد 30 شته بالغ از هر گونه پس از 24 ساعت تغذیه گیرش روی پرشین لایم آلوده به ویروس، به مدت 24 و 48 ساعت برای تغذیه دهش روی گیاهچه‌های اورکالمون (Citrus limon cv. Eureka) منتقل شدند. شش ماه پس از انتقال، شمارش گیاهان دارای علایم تفکیک انجام شد و آلودگی آنها با آزمون آر.تی.پی.سی.آر دو مرحله‌ای مورد تائید قرار گرفت. در آزمون اول میزان انتقال ویروس در مدت 24 ساعت تغذیه گیرش و دهش ویروس برای شته سبز مرکبات، جالیز و سیاه مرکبات به ترتیب 67/16، 33/13 و 67/26 درصد و در مدت 24 ساعت تغذیه گیرش و 48 ساعت تغذیه دهش ویروس به ترتیب 33/23، 20 و 33/33 درصد محاسبه شد. با افزایش مدت زمان تغذیه شته‌ها از گیاهان گیرنده ویروس از 24 ساعت به 48 ساعت، میزان انتقال افزایش یافت. در آزمون دوم با مدت زمان نامعلوم تغذیه گیرش توسط هر سه گونه شته روی درختان پرشین‌لایم آلوده به ویروس در باغ تحت شرایط طبیعی و 24 و 48 ساعت تغذیه دهش روی اورکالمون سالم در گلخانه، مشخص شد که شته‌های مورد آزمایش قابلیت انتقال ویروس را داشتند. مطالعه حاضر تایید کرد که شته‌های مورد آزمایش، تحت شرایط کنترل شده قادرند ویروس رگبرگ روشنی زرد مرکبات را از پرشین‌لایم به اورکالمون منتقل کنند. | ||
| کلیدواژه‌ها | ||
| اورکالمون؛ پرشین لایم؛ شته؛ ویروس | ||
| عنوان مقاله [English] | ||
| Transmissibility of citrus yellow vein clearing virus by three dominant citrus aphids | ||
| نویسندگان [English] | ||
| Reza Maghsoudi1؛ Saeid Nassrollahnejad1؛ Sirous Aghajanzadeh2؛ Seyed Mehdi Bani Hashemian2 | ||
| 1Department of Plant Protection, Gorgan University of Agricultural Sciences and Natural Resources, Golestan, Iran | ||
| 2Citrus and Subtropical Fruits Research Center, Horticultural Sciences Research Institute, Agricultural Research, Education and Extension Organization, Ramsar, Iran | ||
| چکیده [English] | ||
| Citrus yellow vein clearing virus (CYVCV) is in the genus of Mandarivirus that infects some citrus cultivars and causes damage. In this study, the transmission of CYVCV by Aphis spiraecola, A. gossypii, and Toxoptera aurantii was investigated. Several 30 adult aphids of each species after a 24-hour acquisition access period (AAP) on infected Persian Lime were transferred to Citrus lemon cv. Eureka seedlings for 24 and 48 hours’ inoculation access period (IAP). Six months after inoculation, the infected plants were counted. Then their infection was confirmed by a two-step RT-PCR test. The results of the first test showed that 6 months’ post-inoculation, the mean transmission rate of CYVCV by A. spiraecola, A. gossypii, and T. aurantii was 16.67%, 13.33%, and 26.67%, respectively, for 24h AAP/24h IAP. The mean transmission rate was 23.33%, 20%, and 33.33% for 24h AAP/48h IAP, respectively. By increasing the inoculation access period of aphids on virus-infected plants from 24 h to 48 h, the transmission rate increased. In the second test, all three species of aphids with an unknown AAP were collected from infected Persian lime trees in the orchard, under natural conditions and allowed to feed for 24 and 48 hours on Eureka lemon in the greenhouse. It was found that the tested aphids were capable of transmitting this virus. The present study confirmed that the virus is transmitted from Persian lime to Eureka lemon by these citrus aphids under controlled conditions. | ||
| کلیدواژه‌ها [English] | ||
| Aphid, Eureka lemon, Persian lime, Virus | ||
| مراجع | ||
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