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تأثیر سمیت مزمن حشره کش دیازینون بر شاخصهای رشد، بقاء و فعالیتهای فیزیولوژیکی آرتمیای دریاچة ارومیه (Artemia urmiana) | ||
شیلات | ||
دوره 76، شماره 2، خرداد 1402، صفحه 237-249 اصل مقاله (854.44 K) | ||
نوع مقاله: مقاله پژوهشی | ||
شناسه دیجیتال (DOI): 10.22059/jfisheries.2023.351498.1353 | ||
نویسندگان | ||
سونا قمرشناس1؛ بهروز آتشبار کنگرلوئی* 2؛ کوروش سروی مغانلو3؛ احمد ایمانی3؛ مجتبی پوراحد انزابی4 | ||
1کارشناسی ارشد گروه شیلات و آبزیان، دانشکده منابع طبیعی، دانشگاه ارومیه، ارومیه، ایران | ||
2دانشیار گروه اکولوژی و مدیریت ذخایر آبی، پژوهشکده آرتمیا و آبزیپروری، دانشگاه ارومیه ، ارومیه، ایران | ||
3دانشیار گروه شیلات و آبزیان، دانشکده منابع طبیعی، دانشگاه ارومیه، ارومیه، ایران | ||
4دانشجوی دکتری گروه شیلات و آبزیان، دانشکده منابع طبیعی، دانشگاه ارومیه، ارومیه، ایران | ||
چکیده | ||
مطالعة حاضر با هدف بررسی اثر سمیت مزمن حشرهکش دیازینون بهعنوان یکی از پرمصرفترین سموم مورد استفاده در زمینهای زراعی و باغهای اطراف دریاچة ارومیه، بر شاخصهای رشد، بقاء، فعالیت آنزیمهای آنتیاکسیدانی و گوارشی در گونة Artemia urmiana صورت گرفت. ابتدا LC50 حشرهکش دیازینون برای مراحل مختلف سنی آرتمیا شامل ناپلی، متاناپلی و بالغین در ساعتهای 24، 48 و 96 با استفاده از آزمون پروبیت سنجش شد. LC50 دیازینون برای ناپلی در ساعتهای عنوانشده بهترتیب 23/769، 9920 و 0/008، برای متاناپلی 2/231، 0/015 و 0/002 و برای بالغین 0/919، 0/038 و 0/007 میلیگرم بر لیتر بهدست آمد. برای بررسی اثر سمیت مزمن حشرهکش دیازینون بر شاخصهای زیستی، A. urmiana در قالب 4 تیمار کنترل، 25 درصد LC50، 50 درصد LC50 و 100 درصد LC50 بهمدت 15 روز پرورش داده شد. شاخصهای رشد و درصد بقاء در روزهای 8، 11 و 15 و فعالیت آنزیمهای آنتیاکسیدانی و گوارشی کل زیتودة آرتمیاها در انتهای دوره سنجش شدند. بیشترین مقدار رشد و بقاء در تیمار کنترل و کمترین آنها در تیمار 100 درصد LC50 مشاهده شد (0/05>P). فعالیت آنزیم سوپراکسید دیسموتاز در تیمارهای 25 درصد LC50 و 50 درصد LC50 کاهش یافت (0/05>P). فعالیت آنزیمهای کاتالاز و گلوتاتیون پراکسیداز نیز در تیمارهای 25 درصد LC50 و 50 درصد LC50 در مقایسه با تیمار 100 درصد LC50 کاهش یافت (0/05>P). بین تیمارهای مختلف در فعالیت آنزیمهای آلکالین پروتئاز و لیپاز اختلاف معنیداری مشاهده نشد (0/05<P). فعالیت آنزیم آلفا آمیلاز با افزایش غلظت دیازینون کاهش یافت (0/05>P). بهطور کلی میتوان نتیجه گرفت که دیازینون باعث کاهش رشد و بقاء در A. urmiana خواهدشد و اثرات منفی بر فعالیت آنزیمی دارد. با توجه به نتایج پژوهش حاضر جهت حفظ این گونه، استفاده از حشرهکش دیازینون در مناطق اطراف دریاچه ارومیه بایستی کنترل شده باشد. | ||
کلیدواژهها | ||
دیازینون؛ رشد؛ بقاء؛ آنزیمهای آنتیاکسیدانی و گوارشی؛ Artemia urmiana | ||
عنوان مقاله [English] | ||
Effect of chronic toxicity of diazinon insecticide on growth, survival and physiological activities of Urmia Lake Artemia (Artemia urmiana) | ||
نویسندگان [English] | ||
Sona Ghamarshenas1؛ Behrooz Atashbar Kangarloei2؛ Kourosh Sarvi Moghanlou3؛ Ahmad Imani3؛ Mojtaba Pourahad Anzabi4 | ||
1M.Sc. Graduate, Department of Fisheries, Faculty of Natural Resources, University of Urmia, Urmia, Iran | ||
2Departeant of Ecology and Resource Assessment, Urmia Lake Research Institute,, Uremia University, Urmia, Iran | ||
3Associate Professor, Department of Fisheries, Faculty of Natural Resources, University of Urmia, Urmia, Iran | ||
4Ph.D. Student, Department of Fisheries, Faculty of Natural Resources, University of Urmia, Urmia, Iran | ||
چکیده [English] | ||
This study was carried out with the aim of investigating the effect of diazinon insecticide chronic toxicity as one of the most widely used poisons used in agricultural lands and gardens around Lake Urmia on growth, survival, antioxidant and digestive enzyme activity indices of Artemia urmiana. First, the LC50 of diazinon insecticide for different age stages, including nauplii, post larvae and adults, were measured at 24, 48 and 96 hours using the probit analysis. The LC50 of diazinon for nauplii 23.769, 0.992, 0.008, post larvae 2.231, 0.015, 0.002 and adults 0.919, 0.038, 0.007 (mg/l) were obtained. To investigate the effect of chronic toxicity of diazinon, A. urmiana was cultured in the form of 4 treatments, control, 25% LC50, 50% LC50 and 100% LC50 for 15 days. The growth and survival indices were measured on days 8, 11 and 15, and the activity of antioxidant and digestive enzymes of the biomass were measured at the end of the period. The highest amount of growth and survival was observed in the control treatment and the lowest in the 100% LC50 treatment (P<0.05). The activity of superoxide dismutase decreased in 25% LC50 and 50% LC50 treatments (P<0.05). The activities of catalase and glutathione peroxidase enzymes also decreased in the 25% LC50 and 50% LC50 treatments compared to the 100% LC50 treatment (P>0.05). There was no significant difference between different treatments in the activity of alkaline protease and lipase enzymes (P<0.05). Alpha-amylase enzyme activity decreased with increasing diazinon concentration (P>0.05). In general, it can be concluded that diazinon reduces growth and survival in A. urmiana and has negative effects on enzyme activities. According to the results of the present research, in order to preserve this species, the use of diazinon insecticide in the areas around Lake Urmia should be controlled. | ||
کلیدواژهها [English] | ||
Diazinon, Growth, Survival, Antioxidant and Digestive enzymes, Artemia urmiana | ||
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