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شناسایی نواحی ژنومی تحت انتخاب مثبت برای سازگاری با ارتفاع بالا در گوسفندان ایرانی | ||
علوم دامی ایران | ||
دوره 55، شماره 1، فروردین 1403، صفحه 143-162 اصل مقاله (1.86 M) | ||
نوع مقاله: مقاله پژوهشی | ||
شناسه دیجیتال (DOI): 10.22059/ijas.2023.353223.653925 | ||
نویسندگان | ||
زهرا پتی آبادی1؛ محمد رزم کبیر* 1؛ علی اسمعیلی زاده کشکوئیه2؛ محمدحسین مرادی3؛ امیر رشیدی4 | ||
1گروه علوم دامی، دانشکده کشاورزی، دانشگاه کردستان، سنندج، ایران | ||
2گروه علوم دامی، دانشکده کشاورزی، دانشگاه شهید باهنر کرمان، کرمان، ایران | ||
3گروه علوم دامی، دانشکده کشاورزی، دانشگاه اراک، اراک، ایران | ||
4گروه علوم دامی، دانشکده کشاورزی، دانشگاه کردستان، سنندج، ایران. | ||
چکیده | ||
شناسایی نشانههای انتخاب، اطلاعاتی در مورد مراحل تکامل گونههای مختلف از جمله گوسفند، که منجر به تغییراتی در سطح ژنوم آنها در طی سالیان متمادی میشود، را فراهم میکند. هدف از این پژوهش شناسایی نشانههای ژنومی انتخاب در گوسفندان مناطق مرتفع کشور ایران بود. بدین منظور 58 رأس گوسفند از 4 نژاد که دو نژاد در مناطق مرتفع و دو نژاد در مناطق پست پراکندهاند، با استفاده از آرایههای گوسفندk 600 تعیین ژنوتیپ شدند. جهت شناسایی نواحی ژنومی تحت انتخاب از دو آزمون آماری برآوردگر نااریب FST (تتا) و hapFLK استفاده شد. با استفاده از آزمون تتا، 22 منطقه ژنومی به عنوان مناطق تحت انتخاب شناسایی شدند. در آزمون hapFLK نیز 15 منطقه ژنومی شناسایی شد. تجزیه و تحلیل اطلاعات زیستی (بیوانفورماتیکی) نشان داد که برخی از این مناطق ژنومی با ژنهای موثر بر سازگاری با شرایط کم اکسیژنی (TXNDC5)، خونسازی (FANCA)، ایمنیزایی و مقابله با عفونت (LEF1، NMUR1، PTMA و COPS7B)، تنظیم فشار خون و پاسخ به درد و التهاب (NMUR1) و سرکوب سرطان (CD82، GAS8، PRMT1، B3GNT7 و ...) همپوشانی دارند. بررسی QTLهای گزارش شده نیز نشان داد که این مناطق با QTLهای صفات مهم اقتصادی از جمله صفات مرتبط با میانگین غلظت هموگلوبین گلبولهای قرمز، هماتوکریت، مشخصات گوشت، لاشه، شیر، وزن بدن، تراکم استخوان و تعداد کل برههای متولد شده در ارتباط میباشند. با توجه به اینکه تحقیقات کمی در رابطه با سازگاری با ارتفاع در گوسفندان انجام شده است، لذا نتایج این تحقیق میتواند اطلاعات سودمندی در رابطه با ژنهایی که به سازگاری با ارتفاع پاسخ میدهند، ارایه دهد. به هر حال، به منظور شناسایی و بررسی دقیقتر جهشهای عامل در ژنها و QTLهای شناسایی شده ضروری است که پژوهشهای تکمیلی بیشتری صورت گیرد. | ||
کلیدواژهها | ||
آزمون FST؛ آزمون hapFLK؛ ارتفاع از سطح دریا؛ پویش ژنومی؛ نشانههای انتخاب | ||
عنوان مقاله [English] | ||
Detection of genomic regions under positive selection in adapting to high altitude in Iranian sheep | ||
نویسندگان [English] | ||
Zahra Patiabadi1؛ Mohammad Razmkabir1؛ Ali Esmailizadeh2؛ Mohammad Hossein Moradi3؛ Amir Rashidi4 | ||
1Department of Animal Science, Faculty of Agriculture, University of Kurdistan, Sanandaj, Iran. | ||
2Department of Animal Science, Faculty of Agriculture, Shahid Bahonar University of Kerman, Kerman, Iran | ||
3Department of Animal Science, Faculty of Agriculture, University of Arak, Arak, Iran | ||
4Department of Animal Science, Faculty of Agriculture, University of Kurdistan, Sanandaj, Iran. | ||
چکیده [English] | ||
Selective signatures provide information about the stages of evolution of different species, including sheep, which lead to changes in their genome over many years. The aim of this study was to detect signatures of selection in the genome of Iranian sheep in the highlands. A total of 58 sheep from 4 breeds, two breeds scattered in the highlands and two breeds scattered in the lowlands, were genotyped using Illumina ovine SNP600K BeadChip genomic arrays. Two statistical tests of unbiased FST (Theta) and hapFLK were used to identify the selection signatures. The results of Theta revealed 22 genomic regions and the results of hapFLK revealed 15 genomic regions. Bioinformatics analysis demonstrated that many of genes had important effects on adaptation to hypoxia conditions e.g. rheumatoid arthritis (TXNDC5), hematopoiesis (FANCA), immunity and fighting infection (LEF1, NMUR1, PTMA and COPS7B), regulating blood pressure and responding to pain and inflammation (NMUR1) and suppressing cancer (CD82, GAS8, PRMT1, B3GNT7). For example, TXNDC5 and FANCA functional genes, which are located on chromosome 13 and 14, were related to reacts to hypoxic conditions and hematopoiesis. Also, genes such as LEF1, NMUR1, PTMA and COPS7B are effective in immunogenicity and fighting infection. Study of the reported QTL in these regions of the sheep genome showed that they overlapped with QTL of economically important traits such as corpuscular hemoglobin concentration, hematocrit, traits related to meat, carcass, milk, body weight, bone density, and the total number of lambs born. Due to the fact that little research has been done regarding adaptation to altitude in sheep, the results of this research may facilitate the identification of genes affecting adaptation to altitude. However, it will be necessary to carry out more association and functional studies to demonstrate the implication of these genes. | ||
کلیدواژهها [English] | ||
Selection Signature, altitude, Genomic scanning, FST test, hapFLK test | ||
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