پیوندهای مفید
اخبار و اعلانات
آمار
| تعداد نشریات | 162 |
| تعداد شماره‌ها | 6,579 |
| تعداد مقالات | 71,072 |
| تعداد مشاهده مقاله | 125,681,126 |
| تعداد دریافت فایل اصل مقاله | 98,911,443 |
بررسی اثر سطوح مختلف میوۀ بلوط بر ساختار جمعیت و تنوع ژنتیک باکتریایی اپیمورال شکمبه با استفاده از تکنیک PCR-SSCP در بز مرخز | ||
| علوم دامی ایران | ||
| دوره 55، شماره 2، تیر 1403، صفحه 245-257 اصل مقاله (1020.69 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22059/ijas.2023.356066.653936 | ||
| نویسندگان | ||
| بدری امیری* ؛ عثمان عزیزی | ||
| گروه علوم دامی، دانشکده علوم کشاورزی، دانشگاه کردستان، کردستان، ایران | ||
| چکیده | ||
| هدف از پژوهش حاضر، بررسی اثرات سطوح مختلف میوۀ بلوط بر تنوع زیستی جمعیت باکتریایی اپیمورال شکمبه با استفاده از تکنیک مولکولی PCR-SSCP در بزغالههای نژاد مرخز بود. برای این منظور تعداد 24 رأس بزغالۀ مرخز با میانگین وزنی 25/1 ± 93/16 کیلوگرم و میانگین سنی 4 تا 5 ماهه در قالب طرح کاملاً تصادفی با 4 تیمار آزمایشی و 6 تکرار به مدت 105 روز مورد آزمایش قرار گرفتند. تیمارهای آزمایشی شامل 1) جیرۀ شاهد، 2) جیرۀ حاوی 8 درصد میوۀ بلوط، 3) جیرۀ حاوی 17 درصد میوۀ بلوط و 4) جیرۀ حاوی 25 درصد میوۀ بلوط بودند. نتایج نشان داد که تأثیر جیره بر تنوع زیستی جمعیت باکتریایی اپیمورال شکمبه معنیدار بود (001/0P<). بیشترین مقدرا شاخص شانون مربوط به تیمار 8 درصد میوۀ بلوط و کمترین مقدار شاخص شانون مربوط به تیمار شاهد بود که با سایر تیمارها اختلاف معنیداری نشان داد. جایگاه نمونهبرداری تأثیری بر تنوع زیستی جمعیت باکتریایی اپیمورال شکمبه نداشت (05/0P>). بیشترین مقدار شاخص شانون مربوط به جایگاه ونترال شکمبه و کمترین آن مربوط به رتیکولوم بود. اثر متقابل جیره و جایگاه نمونهبرداری بر تنوع زیستی جمعیت باکتریایی اپیمورال شکمبه معنیدار بود (05/0P>). نتایج پژوهش حاضر نشان داد که استفاده از میوۀ بلوط تا سطح 17 درصد مادۀ خشک در جیرۀ غذایی موجب افزایش در تنوع زیستی جمعیت باکتریایی اپیمورال شکمبه شد، در حالیکه استفاده از سطح 25 درصد آن در جیرۀ غذایی کاهش تنوع زیستی در جمعیت باکتریایی اپیمورال شکمبه را به دنبال داشت. | ||
| کلیدواژه‌ها | ||
| بزغاله های مرخز؛ تنوع زیستی جمعیت باکتریایی؛ اپیمورال شکمبه؛ میوۀ بلوط؛ PCR-SSCP | ||
| عنوان مقاله [English] | ||
| Effect of different levels of oak acorn on biodiversity bacterial epimural populations using molecular techniques of PCR-SSCP in Markhoz goats | ||
| نویسندگان [English] | ||
| Badri Amiri؛ Osman Azizi | ||
| Department of Animal Science, Faculty of Agricultural Sciences, University of Kurdistan, Kurdistan, Iran. | ||
| چکیده [English] | ||
| The aim of this study was to evaluate the effects of different levels of oak Acorn on the biodiversity of rumen epimural bacterial population using PCR-SSCP molecular technique in Merkhoz goats. A total of 24 Markhoz goats with a mean BW of 16.93±1.25 kg and an average age of 4–5 months were tested in a completely randomized design with 4 treatments and 6 replications for 105 days. Experimental treatments included 1) control diet, 2) diet containing 8% oak acorn, 3) diet containing 17% oak acorn and 4) diet containing 25% oak acorn. The results showed that the effect of diet on biodiversity of rumen bacterial epimural community was significant (P <0.001). There was no significant difference between other experimental treatments (P> 0.05), although treatments containing 8% and 17% oak had more variety than treatments containing 25% oak. Sampling site had no effect on biodiversity of rumen bacterial epimural community (P> 0.05). The highest value of Shannon index was related to ventral ruminal site and the lowest was related to reticulum. The interaction effect of diet and sampling position on biodiversity of rumen bacterial epimural community was significant (P> 0.05). The results showed that the use of oak acorn up to 17% in the diet increased the biodiversity of the of rumen bacterial epimural community, while the use of 25% oak in the diet decreased the biodiversity of the rumen bacterial epimural community. | ||
| کلیدواژه‌ها [English] | ||
| Bacterial population, Biodiversity, Markhoz goats, Oak Acorn, Rumen epimural, PCR-SSCP | ||
| مراجع | ||
|
Cheng, K. J., Fay, J. P., Coleman, R. N., Milligan, L. P., & Costerton, J. W. (1981). Formation of bacterial microcolonies on feed particles in the rumen. Journal of Applied and Environmental Microbiology, 41, 298-305. Donovan, L. O., & Brooker, J. D. (2001). Effect of hydrolysable and condensed tannins on growth, morphology and metabolism of Streptococcus gallolyticus (S. caprinus) and Streptococcus bovis. Journal of Microbiology, 4, 1025-1033. Ebrahimi, A., Khayami, M., & Nejati, V. (2009). Evaluation of antibacterial activity of hydroalcoholic extract of Iranian oak fruit by diffusion method. Journal of Medicinal Plants Quarterly, 9, 34-26. (In Persian). Ephraim, E., Odenyo, A., & Ashenafi, M. (2005). Screening for tannin degradation by rumen and faecal samples of wild and domestic animals in Ethiopia. Journal of Microbiology and Biotechnology, 21, 803-809. Firkins, J. L. (2010). Reconsidering rumen microbial consortia to enhance feed efficiency and reduce environmental impact of ruminant livestock production systems. Journal of Revista Brasileira de Zootecnia, 39, 445-457. Ghaderi, Q, M., Mahonak, A. S., Aalmi, M., Victim, M., & Azizi, M. H. (2011). Evaluation of anti-radical activity, regenerative power and antioxidant capacity of phenolic extract of an oak variety (Q.branti var. Persica). Journal of Food Industry Research, 1, 104-93. (In Persian). Jany, J. L., & Georges, B. (2008). Culture-Independent methods for dentifying microbial communities in cheese. Journal of Food Microbiology, 25, 839-848. Jenkins, T. C., Wallace, R. J., Moate, P. J., & Mosley, E. E. (2007). Board-Invited review: recent advances in biohydrogenation of unsaturated fatty acids within the rumen microbial ecosystem. Journal of Animal Scienc, 86, 397-412. Jill E. C. (2004). Impact of 16S rRNA gene sequence analysis for identification of bacteria on clinical microbiology and infectious diseases. Journal of Clinical Microbiology Reviews, 17, 840-862. Kamra D. N. 2005. Rumen microbial ecosystem. Journal of Current Science, 89, 124-135. Lawrence, G., Gunton, J., Turenne, Ch. Y., Wolfe, J., & Kabani, A. M. (2001). Identification of mycobacterium species by multiple-fluorescence PCR–single-strand conformation polymorphism analysis of the 16S rRNA gene. Journal of Clinical Microbiology, 39, 3085–3091. Lee, D. H., Gunzo. Y., & Kim, S. J. (1996). Nonradioactive Method to Study Genetic Profiles of Natural Bacterial Communities by PCR–Single-Strand-Conformation Polymorphism. Journal of Applied and Environmental Microbiology, 62, 3112-3120. Lorna, J. M., & Jones, G. A. (1981). Isolation and presumptive identification of adherent epithelial bacteria ("epimural" bacteria) from the ovine rumen wall. Journal of Applied and Environmental Microbiology, 41, 1020-1028. Lukas, F., Simunek, J., Mrazek, J,. & Kopecny, J. (2010). PCR-DGGE analysis of bacterial population attached to the bovin rumen wall. Journal of Folia Microbiol, 55, 345-348. Mcallister, T. A., Bae, H. D., Jones, G. A., & Cheng, K. J. (1994). Microbial attachment and feed digestion in the rumen. Journal of Animal Scienc, 72, 3004-3018. Mccowan, R. P., Cheng, K. J., & Costerton, J. W. (1980). Adherent bacterial populations on the bovine rumen wall: distribution patterns of adherent bacteria. Journal of Applied and Environmental Microbiology, 39, 233-241. McSweeney, C.S., Palmer, B., McNeill, D.M. & Krause, D.O. 2001. Microbial interactions with tannins: nutritional consequences for ruminants. Journal of Animal Feed Science and Technoogy, 91: 83-93. Mehansho, H., Asquith, T. N., Butler, L. G., Rogler, J. C., & Carlson, D. M. (1992). Tannin-mediated induction of proline-rich protein synthesis. Journal of Agricultural and Food Chemistry, 40(1), 93-97. Muetzel, S., & Becker, K. (2006). Extractability and biological activity of tannins from various tree leaves determined by chemical and biological assays as affected by drying procedure. Journal of Animal Feed Science and Technology, 125, 139-149. Molan, A.L., Hoskin, S.O., Barry, T.N., & McNabb, W.C. 2000. Effect of condensed tannins extracted from four forages on the viability of the larvae of deer lungworms and gastrointestinal nematodes. Journal of The Veterinary Record, l47: 44-48. Nishitani, Y., & Osawa, R. (2005). Deceptive Halo Formation by Tannas- Defective Bacteria on Tannin-Treated Plate Media. Journal of Microbes Environment, 20(2), 117-119. Nocker, A, Burr, M., & Camper, A, K. (2007). Genotypic microbial community profiling: a critical technical review. Journal of Microbial Ecology, 54, 276-289. Patra, A. K., & Saxena, J. (2009a). The effect and mode of action of saponins on the microbial populations and fermentation in the rumen and ruminant production. Journal of Nutrition Research Reviews, 22, 204–219. Patra, A. K., & Saxena, J. (2009b). Dietary phytochemicals as rumen modifiers: a review of the effects on microbial populations. Journal of Antonie van Leeuwenhoek, 96, 363–375. Patra, A. K., & Saxena, J. (2010). A new perspective on the use of plant secondary metabolites to inhibit methanogenesis in the rumen. Journal of Phytochemistry, 71,1198–1222. Pitta, D., Pinchak, W. W. E., Dowd, S. E., Osterstock, J., Gontcharova, V., Youn, E., Dorton, K., Yoon, I., Min, B. R., Fulford, J. D., Wickersham, T. A., & Malinowski. D. P. (2010). Rumen bacterial diversity dynamics associated with changing from bermudagrass hay to grazed winter wheat diets. Journal of Microbial Ecology, 59, 511–522. Ranilla, M. J., Garcia, A. I. M., Alcaide, E. M., & Carro, M. D. (2009). Analysis of microbial communities in rusitec and single- flow continuous culture fermenters by PCR-SSCP: effects of basal dite. Journal of Options Mediterraneennes, 85, 239- 243. Rosales, R. B. (1999). Condensed tannins in tropical forage legumes: their characterisation and study of their nutritional impact from the standpoint of structure-activity relationships. Department of Agriculture, the University of Reading. Russell, J. B., Muck, E. R., & Weimer, P. J. (2008). Quntitative analysis of cellulose degradation and growth of celluloly tic bacteria in the rumen. Journal of FEMS Microbiology Ecology, 67, 183-197. Sadet, S., Martin, C., Meunier, B., & Morgavi, D. P. (2007). PCR-DGGE analysis revelas a distinct diversity in the bacterial population attached to the rumen epithelium. Journal of Animal Bioscience, 1, 939-944. Sadet, S., Martin, C., & Morgavi, D. P. (2010). Bacterial diversity dynamics in rumen epithelium of wethers fed forage and mixed concentrate forage diets. Journal of Veterinary Microbiology, 146, 98–104. SAS. (2002). User's guide: Statistics, Version 9.1. SAS Institute, Inc. Cary, NC, USA. 210–217. Tajima, K., Aminov, R. I., Nagamine, T., Ogata, K., Nakamura, M., Matsui, H., & Benno, Y. 1999. Rumen bacterial diversity as determined by sequence analysis of 16S rDNA libraries. Journal of FEMS Microbiology Ecology, 29: 159-169. | ||
|
آمار تعداد مشاهده مقاله: 200 تعداد دریافت فایل اصل مقاله: 238 |
||
سامانه مدیریت نشریات علمی. قدرت گرفته از سیناوب