اثر همزمان تمرین هوازی و مصرف عصارۀ سبوس برنج بر بیان ژن‌های التهابی بافت چربی سفید در رت‌های مادۀ چاق

معظمی گودرزی, مجتبی; غلامرضایی دارسرا, شهرام; آذربایجانی, محمدعلی; علمیه, علیرضا

doi:10.22059/jsb.2023.354829.1578

	اثر همزمان تمرین هوازی و مصرف عصارۀ سبوس برنج بر بیان ژن‌های التهابی بافت چربی سفید در رت‌های مادۀ چاق
نشریه علوم زیستی ورزشی
دوره 15، شماره 4، دی 1402، صفحه 5-20
نوع مقاله: مقاله پژوهشی Released under CC BY-NC 4.0 license I Open Access I
شناسه دیجیتال (DOI): 10.22059/jsb.2023.354829.1578
نویسندگان
مجتبی معظمی گودرزی¹؛ شهرام غلامرضایی دارسرا^* ²؛ محمدعلی آذربایجانی³؛ علیرضا علمیه¹
¹گروه تربیت بدنی، واحد رشت، دانشگاه آزاد اسلامی، رشت، ایران
²نویسنده مسئول، گروه تربیت بدنی، واحد رشت، دانشگاه آزاد اسلامی، رشت، ایران.
³گروه فیزیولوژی ورزشی، واحد تهران مرکز، دانشگاه آزاد اسلامی، تهران، ایران
چکیده
مقدمه: چاقی و التهاب یک ریسک‌فاکتور مهم بیماری‌هاست. تمرینات هوازی (AE) و گیاهان دارویی می‌توانند التهاب ناشی از چاقی را کاهش دهند. هدف از این تحقیق بررسی تأثیر AE و سبوس برنج (RBE) بر بیان ژن‌های التهابی در بافت چربی سفید موش‌های صحرایی مادۀ چاق بود. روش پژوهش: 30 موش صحرایی مادۀ ویستار به‌طور تصادفی به پنج گروه کنترل تغذیه‌شده با رژیم غذایی طبیعی، کنترل رژیم غذایی پرچرب (HFD)، HFD+(AE)، HFD+(RBE) و HFD (AE)+(RBE) تقسیم شدند. موش‌ها به مدت چهار هفته روی تردمیل دویدند و (RBE) دریافت کردند. به‌منظور القای چاقی از روغن سرخ‌کردنی پالم استفاده شد (0.5 ml/g). پس از قربانی‌سازی، بافت چربی سفید برای اندازه‌گیری بیان ژن‌های IL6، TNF-α و TLR4 استخراج شد. یافته‌ها: HFD سبب افزایش معنا‌داری در بیان ژن IL-6، TNF-α و TLR4 شد (P=0.001). تم)رین هوازی بیان ژن‌های IL-6(P=0.001)، TNF-a(P=0.041) و TLR-4(P=0.032) را به‌طور معنا‌داری کاهش داد. عصارۀ سبوس برنج بیان ژن‌های IL-6(P=0.001)،TNF-α (P=0.003) وTLR-4 (P=0.011) را به‌طور معناداری کاهش داد. بیان ژن IL-6(P=0.002)، TNF-α (P=0.005) وTLR4 (P=0.011) در گروهAE + RBE نسبت به گروه کنترل HFD به‌طور معنا‌داری کمتر بود. تعامل AE و RBE بر بیان این ژن‌ها معنادار نبود. نتیجه‌گیری: (AE) و (RBE) بیان ژن واسطه‌های التهابی ناشی از HFD را در بافت چربی سفید کاهش می‌دهند. استفاده از مصرف AE و RBE به‌عنوان یک راه‌حل پیشگیرانه و درمانی در شرایط چاقی توصیه می‌شود.
کلیدواژه‌ها
تمرین هوازی؛ سبوس برنج؛ 6 IL-؛ TNF-a و TLR-4
عنوان مقاله [English]
The Simultaneous Effect of Aerobic Exercise and Rice Bran Extract Consumption on Inflammatory Genes Expression in White Adipose Tissue of Obese Female Rats
نویسندگان [English]
Mojtaba Moazamigoodarzi¹؛ shahram gholamrezaei darsara²؛ Mohammad Ali ََAzarbayjani³؛ Alireza Elmiye¹
¹Department of Physical Education, Rasht Branch, Islamic Azad University, Rasht, Iran
²Department of Physical Education, Rasht Branch, Islamic Azad University, Rasht, Iran
³Department of Exercise Physiology, Central Tehran Branch, Islamic Azad University, Tehran, Iran.
چکیده [English]
Introduction: Obesity and inflammation is an important risk factor for diseases. Aerobic exercise (AE) and medicinal herbs can reduce obesity-induced inflammation. This study aimed to investigate the effect of AE and Rice Bran Extract (RBE) on the inflammatory gene expression in white adipose tissue of obese female rats. Methods: Thirty female Wistar rats were randomly divided into five groups including Control with a natural diet, Control with a High-Fat Diet (HFD), HFD + AE, HFD + RBE, and HFD + AE + RBE. Rats ran on a treadmill for four weeks and received RBE. To induce obesity, frying palm oil was used (0.5 ml/g). After euthanasia, the white adipose tissue was extracted to measure the IL6, TNF-α, and TLR4 gene expression. Results: HFD caused a significant increase in the IL-6, TNF-α, and TLR4 gene expression (P=0.001). AE significantly decreased IL-6 (P=0.001), TNF-α (P=0.041), and TLR4 (P=0.032) gene expression. RBE significantly decreased the IL-6 (P=0.001), TNF-α (P=0.003), and TLR-4 (P=0.011) gene expression. IL-6 (P=0.002), TNF-α (P=0.005), and TLR4 (P=0.011) gene expression was significantly decreased in the AE + RBE group compared to the control group with HFD, However, AE and RBE interaction in the expression of these genes was not statistically significant. Conclusion: AE and RBE decrease the gene expression of inflammatory mediators caused by HFD in white adipose tissue. The AE and RBE consumption is recommended as a preventive and therapeutic solution in obesity conditions.
کلیدواژه‌ها [English]
aerobic exercise, rice bran, IL-6, TNF-a and TLR-4

مراجع
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اخبار و اعلانات

آمار

اثر همزمان تمرین هوازی و مصرف عصارۀ سبوس برنج بر بیان ژن‌های التهابی بافت چربی سفید در رت‌های مادۀ چاق

1] Kusminski CM, Bickel PE, Scherer PE. Targeting adipose tissue in the treatment of obesity-associated diabetes. Nat Rev Drug Discov. 2016;15(9):639-60. DOI: 10.1038/nrd.2016.75. https://www.ncbi.nlm.nih.gov/pubmed/27256476.

[2] Yudkin JS. Inflammation, obesity, and the metabolic syndrome. Horm Metab Res. 2007;39(10):707-9. DOI: 10.1055/s-2007-985898.

https://www.ncbi.nlm.nih.gov/pubmed/17952830.

[3] Kawai T, Autieri MV, Scalia R. Adipose tissue inflammation and metabolic dysfunction in obesity. Am J Physiol Cell Physiol. 2021;320(3):C375-C91. DOI: 10.1152/ajpcell.00379.2020. https://www.ncbi.nlm.nih.gov/pubmed/33356944.

[6] Nyati KK, Masuda K, Zaman MM, Dubey PK, Millrine D, Chalise JP, et al. TLR4-induced NF-kappaB and MAPK signaling regulate the IL-6 mRNA stabilizing protein Arid5a. Nucleic Acids Res. 2017;45(5):2687-703. DOI: 10.1093/nar/gkx064.

https://www.ncbi.nlm.nih.gov/pubmed/28168301.

[7] Rocha DM, Caldas AP, Oliveira LL, Bressan J, Hermsdorff HH. Saturated fatty acids trigger TLR4-mediated inflammatory response. Atherosclerosis. 2016;244:211-5. DOI: 10.1016/j.atherosclerosis.2015.11.015.

https://www.ncbi.nlm.nih.gov/pubmed/26687466.

[8] Bradley RL, Jeon JY, Liu FF, Maratos-Flier E. Voluntary exercise improves insulin sensitivity and adipose tissue inflammation in diet-induced obese mice. Am J Physiol Endocrinol Metab. 2008;295(3):E586-94. DOI: 10.1152/ajpendo.00309.2007.

https://www.ncbi.nlm.nih.gov/pubmed/18577694.

[13] Sharif MK, Butt MS, Anjum FM, Khan SH. Rice bran: a novel functional ingredient. Crit Rev Food Sci Nutr. 2014;54(6):807-16. DOI: 10.1080/10408398.2011.608586. https://www.ncbi.nlm.nih.gov/pubmed/24345050.

[14] Minatel IO, Francisqueti FV, Correa CR, Lima GP. Antioxidant Activity of gamma-Oryzanol: A Complex Network of Interactions. Int J Mol Sci. 2016;17(8). DOI: 10.3390/ijms17081107. https://www.ncbi.nlm.nih.gov/pubmed/27517904.

[15] Sohail M, Rakha A, Butt MS, Iqbal MJ, Rashid S. Rice bran nutraceutics: A comprehensive review. Crit Rev Food Sci Nutr. 2017;57(17):3771-80. DOI: 10.1080/10408398.2016.1164120. https://www.ncbi.nlm.nih.gov/pubmed/27015585.

[16] Ghatak SB, Panchal SJ. Investigation of the immunomodulatory potential of oryzanol isolated from crude rice bran oil in experimental animal models. Phytother Res. 2012;26(11):1701-8. DOI: 10.1002/ptr.4627. https://www.ncbi.nlm.nih.gov/pubmed/22407738.

[17] Candiracci M, Justo ML, Castano A, Rodriguez-Rodriguez R, Herrera MD. Rice bran enzymatic extract-supplemented diets modulate adipose tissue inflammation markers in Zucker rats. Nutrition. 2014;30(4):466-72. DOI: 10.1016/j.nut.2013.09.016.

https://www.ncbi.nlm.nih.gov/pubmed/24607304.

[18] Justo ML, Claro C, Vila E, Herrera MD, Rodriguez-Rodriguez R. Microvascular disorders in obese Zucker rats are restored by a rice bran diet. Nutr Metab Cardiovasc Dis. 2014;24(5):524-31. DOI: 10.1016/j.numecd.2013.10.032.

https://www.ncbi.nlm.nih.gov/pubmed/24361072.

[20] Qin F, Dong Y, Wang S, Xu M, Wang Z, Qu C, et al. Maximum oxygen consumption and quantification of exercise intensity in untrained male Wistar rats. Sci Rep. 2020;10(1):11520. DOI: 10.1038/s41598-020-68455-8.

https://www.ncbi.nlm.nih.gov/pubmed/32661254.

[22] Seneviratne KP, Anjali NVP, Senanayake CM, Jayathilaka N, Seneviratne KN. Ethanolic extract of rice bran: a thermally stable preservative for edible oils and cake. Food Production, Processing and Nutrition. 2022;4(1):14. DOI: 10.1186/s43014-022-00094-0.

https://doi.org/10.1186/s43014-022-00094-0.

[23] Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods. 2001;25(4):402-8. DOI: 10.1006/meth.2001.1262. https://www.ncbi.nlm.nih.gov/pubmed/11846609.

[24] Zubel-Lojek J, Oclon E, Latacz A, Pierzchala-Koziec K. Adipose Tissue Tumor Necrosis Factor-alpha. and Interleukin-6 Response to Glucocorticoid Treatment during Inflammation. Folia Biol (Krakow). 2016;64(3):205-12.

https://www.ncbi.nlm.nih.gov/pubmed/29847081.

[25] Kiran S, Rakib A, Kodidela S, Kumar S, Singh UP. High-Fat Diet-Induced Dysregulation of Immune Cells Correlates with Macrophage Phenotypes and Chronic Inflammation in Adipose Tissue. Cells. 2022;11(8). DOI: 10.3390/cells11081327.

https://www.ncbi.nlm.nih.gov/pubmed/35456006.

[26] Shi H, Kokoeva MV, Inouye K, Tzameli I, Yin H, Flier JS. TLR4 links innate immunity and fatty acid-induced insulin resistance. J Clin Invest. 2006;116(11):3015-25. DOI: 10.1172/JCI28898. https://www.ncbi.nlm.nih.gov/pubmed/17053832.

[27] Han MS, White A, Perry RJ, Camporez JP, Hidalgo J, Shulman GI, et al. Regulation of adipose tissue inflammation by interleukin 6. Proc Natl Acad Sci U S A. 2020;117(6):2751-60. DOI: 10.1073/pnas.1920004117.

https://www.ncbi.nlm.nih.gov/pubmed/31980524.

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