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بررسی اثر حفاظت فراسرد بر شاخص‌های رشد و ترکیب بیوشیمیایی ریزجلبک Chaetoceros calcitrans | ||
| شیلات | ||
| دوره 76، شماره 3، مهر 1402، صفحه 437-453 اصل مقاله (1.22 M) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22059/jfisheries.2023.358465.1383 | ||
| نویسندگان | ||
| مرتضی بهره مند1؛ محمدعلی نعمت اللهی* 2؛ سجاد پورمظفر3 | ||
| 1دانشجوی دکتری گروه شیلات، دانشکده منابع طبیعی، دانشگاه تهران، کرج، ایران | ||
| 2استادگروه شیلات، دانشکده منابع طبیعی، دانشگاه تهران، کرج، ایران | ||
| 3استادیار پژوهشی ایستگاه تحقیقات نرمتنان خلیج فارس، پژوهشکده اکولوژی خلیج فارس و دریای عمان، موسسه تحقیقات علوم شیلاتی کشور، سازمان تحقیقات، آموزش و ترویج کشاورزی، بندرلنگه، ایران | ||
| چکیده | ||
| در این مطالعه به‌منظور ارزیابی امکان نگهداری ریزجلبک کتوسروس Chaetoceros calcitrans در شرایط فراسرد، 4 عامل محافظت کنندة سرمایی دی متیل سولفوکساید (DMSO)، متانول (ME)، گلیسرول (GL) و اتیلن گلیکول (ET)، هر یک با غلظت‌های مختلف (2/5، 5، 10، 15، 20، 25 درصد (حجم/حجم)، به‌عنوان تیمارهای آزمایشی در نظر گرفته شد و نمونه‌های ریزجلبکی پس از آماده‌سازی به‌مدت 30 روز در دمای 196- درجة سانتی‌گراد قرار داده شد. در ادامه، به‌منظور تعیین بهترین تیمارها کشت مجدد ریزجلبک انجام و عملکرد رشد، ترکیب بیوشیمیایی و پروفیل اسید چرب مورد سنجش قرار گرفت. از 24 تیمار مورد آزمایش، در 15 تیمار پس از خروج از حالت انجماد رشد مجدد سلول‌ها مشاهده شد. بیشترین میزان تراکم نهایی (106×4/13 سلول در میلی‌لیتر) در بین تیمارهای آزمایشی در تیمار DMSO15 مشاهده شد. شاخص بقا در تیمارهایی که پس از انجماد‌زدایی رشد مجدد سلول‌ها در آن‌ها مشاهده شد در دامنه‌ای بین 8/26 تا 28/19 درصد قرار داشت. بیشترین میزان زنده‌مانی به‌ترتیب در تیمارهای DMSO15، DMSO10 و ME15 ثبت شد. سنجش ترکیب بیوشیمیایی نشان داد که تنها در شاخص چربی اندازه‌گیری شده در تیمارهای آزمایشی نسبت به گروه شاهد افزایش معنی‌دار مشاهده شد (0/05>P). سنجش پروفیل اسیدهای چرب نیز هیچ گونه اختلاف معنی‌داری بین تیمارها نشان نداد. نتایج حاصل از پژوهش حاضر نشان داد که حفاظت فراسرد ریزجلبک کتوسروس در شرایطی می‌تواند موفقیت‌آمیز باشد که از DMSO در سطح 5 تا 15 درصد و یا ME در سطح 10 تا 15 درصد به‌عنوان عامل محافظت‌کنندة سرمایی استفاده شود، و مناسب‌ترین نتایج نیز در زمان استفاده از DMSO در سطح 15 درصد به‌دست آمد. | ||
| کلیدواژه‌ها | ||
| حفاظت فراسرد؛ Chaetoceros calcitrans؛ پروفیل اسید چرب؛ DMSO؛ دیاتومه | ||
| عنوان مقاله [English] | ||
| Cryopreservation of the microalgae Chaetoceros calcitrans and its effects on growth and biochemical composition | ||
| نویسندگان [English] | ||
| Morteza Bahremand1؛ Mohammad Ali Nematollahi2؛ Sajjad Pourmozaffar3 | ||
| 1Ph.D. Student, Department of Fisheries, Faculty of Natural Resources, University of Tehran, Karaj, Iran | ||
| 2Professor, Department of Fisheries, Faculty of Natural Resources, University of Tehran, Karaj, Iran | ||
| 3Assistant Professor, Persian Gulf Mollusks Research Station, Persian Gulf and Oman Sea Ecology Research Center, Iranian Fisheries Science Research Institute, Agricultural Research Education and Extension Organization (AREEO), Bandar-e-Lengeh, Iran | ||
| چکیده [English] | ||
| In this study, in order to evaluate the possibility of cryopreservation of C. calcitrans, 4 cryoprotectant agents (dimethyl sulfoxide, methanol, glycerol and ethylene glycol), each with different concentrations (2.5, 5, 10, 15, 20, 25% (v/v)), were taken as experimental treatments. Then, microalgae samples were placed at -196°C for 30 days. After that, in order to determine the best treatments, re-cultivation of microalgae was carried out and growth performance, biochemical composition and fatty acid profile were measured. After thawing, regrowth of microalgae cells was observed in 15 out of 24 treatments. The highest final cell density (4.13x106 cells/ml) was observed in DMSO15. After thawing, in the treatments in which the cells were reproduced again, the viability ranged between 8.26 and 28.19 percent. Maximum viability index were recorded in DMSO15, DMSO10 and ME15, respectively. Biochemical composition analysis showed that there was a significant increase on the lipid content in the experimental treatments compared to the control group (p<0.05). No significant difference was observed on the fatty acid profile(p>0.05). The results showed that the cryopreservation of C. calcitrans can be successful only if we use DMSO (5-15%) or ME (10-15%) as a cryoprotectant, and the best results are obtained when using DMSO at the level of 15%. | ||
| کلیدواژه‌ها [English] | ||
| Cryopreservation, Chaetoceros calcitrans, fatty acid profile, DMSO, Diatom | ||
| مراجع | ||
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