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تأثیر تزریق نانواکسیدروی بر پاسخ ایمنی، فعالیت آنزیمهای اکسیداتیو پلاسما و بیان ژن اینترلوکین بافت طحال در جوجهگوشتی | ||
علوم دامی ایران | ||
دوره 54، شماره 4، آذر 1402، صفحه 391-402 اصل مقاله (1.1 M) | ||
نوع مقاله: مقاله پژوهشی | ||
شناسه دیجیتال (DOI): 10.22059/ijas.2022.348850.653908 | ||
نویسندگان | ||
محمد کاظمی فرد* ؛ منصور رضایی؛ جوانشیر پالوج؛ زربخت انصاری پیرسرائی؛ عیسی دیرنده | ||
گروه علوم دامی، دانشکده علوم دامی و شیلات، دانشگاه علوم کشاورزی و منابع طبیعی ساری، ساری، مازندران، ایران | ||
چکیده | ||
این آزمایش بهمنظور بررسی تأثیر تزریق نانواکسیدروی بر پاسخایمنی و عملکرد در جوجهگوشتی انجام شد. تعداد 108 قطعه جوجه سویه راس308 در قالب طرح کاملاً تصادفی با سه تیمار در چهار تکرار و نه جوجه در هر تکرار در روز تفریخ با محلول نانواکسیدروی تزریق شدند. تیمارها شامل: 1. شاهد، 2. تزریق 20 میکرولیتر نانواکسیدروی(5/0 میلیگرم نانواکسیدروی در 50 میلیلیتر محلولنمکی)، 3. تزریق 20 میکرولیتر نانواکسیدروی(6/0 میلیگرم نانواکسیدروی در 50 میلیلیتر محلولنمکی). محلول نانواکسیدروی از ناحیه گردن به جوجهها تزریق شد. جیره مورد استفاده برای همه تیمارها جیره استاندارد سویه راس 308 بود. تیتر آنتی بادی آنفلوانزا 14 روز و آزمون حساسیت پوستی، 12 و 24 ساعت پس از واکسیناسیون و سطح فعالیت گلوتاتیون پراکسیداز در تیمار 3 نسبت به سایر تیمارها به طور معنیداری افزایش یافت (05/0P< ) بیانژن اینترلوکین12 در تیمار 2 و 3 بهطور معنیداری بیشتر از تیمار شاهد بود (05/0P< ) وزن نسبی سنگدان در تیمار 3 نسبت به تیمار شاهد و خوراک مصرفی در تیمار 2 و 3 بهطور معنیداری کمتر از تیمار شاهد بود(05/0P<). نتایج نشان داد که تزریق نانواکسیدروی باعث افزایش پاسخ ایمنی عمومی و سلولی، فعالیت آنزیمهای آنتیاکسیدان و بیانژن اینترلوکین12 شد. | ||
کلیدواژهها | ||
اینترلوکین؛ آنفلوانزایمرغی؛ گلوتاتیونپراکسیداز؛ سوپراکسیددیسموتاز | ||
عنوان مقاله [English] | ||
Effect of injection of zinc oxide nano particles on the immune response, oxidative enzyme activity of plasma and gene expression in broilers spleen tissue | ||
نویسندگان [English] | ||
Mohammad Kazemifard؛ Mansour Rezaei؛ جوانشیر پالوج؛ Zarbakht Ansari Pirsaraei؛ Essa Dirandeh | ||
Department of Animal science, Faculty of animal and fisheries sciences, Sari agricultural and natural resources university, Sari, Iran | ||
چکیده [English] | ||
This experiment was conducted to investigate the effect of zinc oxide nanoparticls injection on immune response and performance in broilers. One hundred eight Ross308 strain chicks were injected in a completely randomized design with three treatments in four replicates and nine chicks in each replicate on the day of hatching. The treatments include: 1. Control, 2. Injection of 20 microliters of zinc oxide nanoparticls (0.5 mg of zinc oxide nanoparticls in 50 ml of saline), 3. Injection of 20 microliters of zinc oxide nanoparticles (0.6 mg of zinc oxide nanoparticles in 50 ml of saline). The zinc oxide nanoparticls solution was injected into the chickens from the neck area. The ration used for all treatments was the standard ration of Ross 308 strain. Influenza antibody titer 14 days and CBH test, 12 and 24 hours after vaccination and glutathione peroxidase activity level increased significantly in treatment 3 compared to other treatments (P<0.05). Interleukin 12 gene expression in treatment 2 and 3 was significantly higher than the control treatment (P<0.05). The relative weight of gravel in treatment 3 compared to the control treatment and the feed consumed in treatments 2 and 3 were significantly lower than the control treatment (P<0.05). The results showed that the injection of zinc oxide nanoparticls increased the general and cellular immune response, the activity of antioxidant enzymes and interleukin 12 gene expression. | ||
کلیدواژهها [English] | ||
Interleukin, Avian influenza, Glutathioneproxidase, Superoxide dismutas | ||
مراجع | ||
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