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اثر قارچ بیمارگر Beauveria bassiana روی پروانه مینوز گوجهفرنگیTuta absoluta (Lep.: Gelechiidae) | ||
| کنترل بیولوژیک آفات و بیماری های گیاهی | ||
| دوره 11، شماره 1، تیر 1401، صفحه 43-56 اصل مقاله (1.4 M) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22059/jbioc.2023.366056.324 | ||
| نویسندگان | ||
| زهرا رحمتی حبیب آبادی؛ نفیسه پورجواد* | ||
| گروه گیاهپزشکی، دانشکده کشاورزی، دانشگاه صنعتی اصفهان، اصفهان، ایران | ||
| چکیده | ||
| شبپره مینوز گوجهفرنگی (Lep.: Gelechiidae)(Meyrick) Tuta absoluta یکی از آفات مهم گوجهفرنگی است که خسارات سنگینی در کشتهای گلخانهای و مزرعه ای گوجهفرنگی وارد میکند. در راستای توسعه کنترل بیولوژیک این آفت، در تحقیق حاضر تاثیر قارچ بیمارگر (Hypocreales :Cordycipitaceae) Beauveria bassiana (Bals.) Vuil بر مراحل مختلف زندگی شبپره مینوز گوجهفرنگی در شرایط آزمایشگاهی مورد مطالعه قرار گرفت. در بررسی اثر قارچ بر طول دوره زیستی و درصد مرگومیر مراحل مختلف زندگی شبپره مینوزگوجهفرنگی آزمایشات در 10 تیمار (شاهد و غلظتهای مختلف از کنیدی قارچ) در سه تکرار انجام شد. نتایج نشان داد که مرحله تخم و سپس شفیره نسبت به مراحل لاروی به قارچ حساستر هستند و در مراحل مختلف لاروی، لاروهای سنین پایین نسبت به سایر مراحل لاروی به کاربرد قارچ حساستر هستند. به طوری که غلظت 50 درصد کشنده قارچ برای مراحل مختلف تخم، لارو جوان، لارو مسن و شفیره به ترتیب 104×6، 106×16/1، 108×53/2 و 105×39/3 اندازه گیری شد. همچنین طول دوره لاروی و شفیرگی شبپره مینوز گوجهفرنگی تحت تاثیر کاربرد قارچ قرار گرفت و با افزایش میزان غلظت قارچ، طول دوره لاروی از 31/51 به 12/44 روز و طول دوره شفیرگی از 64/6 به 33/5 روز کاهش یافت. نتایج به دست آمده در بررسی اثر قارچ بر میزان مرگ و میر مراحل مختلف زندگی مینوز نشان داد که با افزایش غلظت قارچ میزان مرگومیر تمام مراحل مختلف آفت افزایش یافت به طوری که در غلظت 1010 کنیدی قارچ میزان مرگ و میر مراحل مختلف تخم، لارو جوان، لارو مسن و شفیره به ترتیب به 65/89، 46/88، 71/60 و 90 درصد رسید. همچنین مشخص شد با افزایش غلظت قارچ در سنین مختلف لاروی با افزایش زمان، نرخ زندهمانی کاهش و میزان زندهمانی با افزایش سن لاروی افزایش یافته است. | ||
| کلیدواژه‌ها | ||
| کنترل بیولوژیک؛ نرخ مرگومیر تجمعی؛ کنیدی قارچ | ||
| عنوان مقاله [English] | ||
| The effect of the pathogenic fungus, Beauveria bassiana on tomato leafminer Tuta absoluta (Lep.: Gelechiidae) | ||
| نویسندگان [English] | ||
| Zahra Rahmati Habibabadi؛ Nafiseh Poorjavad | ||
| Department of Plant Protection, College of Agriculture, Isfahan University of Technology, Isfahan, Iran. | ||
| چکیده [English] | ||
| Tomato leafminer, Tuta absoluta (Meyrick) (Lep.: Gelechiidae) is one of the important pests of tomato which causes heavy damage to tomatoes in the greenhouse and outdoor crops. In order to develop biological control of this pest, in the current study, the effects of Beauveria bassiana on different life stages of tomato leaf miner were evaluated in laboratory conditions. To investigate the effect of fungus on the length and survival rate of different stages of T. absoluta, the experiments were conducted in 10 treatments (control and different concentrations of fungal conidia) in three replications. The results showed that the egg stage and then the pupa are more sensitive to the fungus than the larval stages, and in different larval stages, young larvae are more sensitive to the application of the fungus than other larval stages. So the 50% lethal concentrations (LC50) of fungal conidia for different stages; egg, young larva, old larva and pupa was measured as 6×104, 1.16×106, 2×53.108 and 3.39 ×105 respectively. Also, the length of the larval and pupal periods of T. absoluta was affected by the application of B. bassiana, so that the length of the larval and pupal periods decreased from 51.31 to 44.12 days and from 6.64 to 5.33 days, respectively, by an increase in the concentration of fungal conidia. The results obtained in the investigation of the effect of B. bassiana on the mortality rate of different stages of T. absoluta showed that the mortality rate of all the different stages increased with the increase in the fungal conidia concentrations, so that at a concentration of 1010 conidia, the mortality rate of the different stages; egg, young larvae, old larvae, and pupae reached 89.65, 88.46, 60.71 and 90%, respectively. It was also found that by increasing the concentrations of fungal conidia, the survival rate decreased by passing of time in all stages of T. absoluta, and the survival rate increased with increasing age of larvae. | ||
| کلیدواژه‌ها [English] | ||
| Biological control, Cumulative mortality rate, Fungal conidia | ||
| مراجع | ||
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