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The Effect of Some Nano Plant Extract on Bacteria Producing Biogenic Amines Isolated From Minced Meat | ||
Iranian Journal of Veterinary Medicine | ||
مقالات آماده انتشار، پذیرفته شده، انتشار آنلاین از تاریخ 20 آذر 1402 اصل مقاله (1.07 M) | ||
نوع مقاله: Original Articles | ||
شناسه دیجیتال (DOI): 10.22059/ijvm.2023.362975.1005428 | ||
نویسندگان | ||
Amany Omar Selim1؛ Marwa Magdy Abdel Salam* 2؛ Rasha Nagiub Abdallah Hassan2؛ Gehan Elsaeid Mustafa3؛ Zeinab Abd ELrahman Mahdy1 | ||
1Bacteriology Department,Benha Provincial Laboratory, Animal Health Research Institue,Agriculture Research Center(ARC), Cairo,Egypt. | ||
2Foof Hygiene Deptartment, Benha Provincial Laboratory, Animal Health Research Institute, Agriculture Research Center,Benha, Egypt | ||
3Biochemistry Deptartment, Benha Provincial Laboratory, Animal Health Research Institute, Agriculture Research Center,Benha, Egypt | ||
چکیده | ||
The effect of some Nano plant extract on bacteria producing biogenic amines isolated from minced meat 1 - Abstract:- Background: Biogenic amines are the end products of bacterial decarboxylation of amino acids which occurs as a result of bacterial contamination. Causing a series of problems for human health Objective: - isolated different bacteria that can produce decarboxylase enzymes and trail to control it by using Garlic, Onion and Ginger nano-emulsions. Methods: Isolation and identification of some bacteria producing decarboxylase enzymes from minced meat, investigating the cytotoxicity of nanoparticles by SRB assay, detect the antibacterial effect of Garlic, union and Ginger nano-emulsions 60% on isolated bacteria by MIC method and the effect on biogenic amines levels by HPLC Results: The (Salmonella species (“Salmonella Typhimurium1, 4{5}, 12: i: 1.2 and S. Arizonae”), E. coli “serotype O44:K74 and O125:K70”, Klebsiella pneumonia, Enterobacter spp, S. aureus, Aeromonas hydrophila, Proteus mirabilis, Pasteurella multocida and Lactobacillus species) were the most isolated bacteria. The biogenic amines that were detected on positive samples were Putrescine, Cadaverine, Spermidine, Spermine, Putrescine , B-phenyl ethyl amine, Histamine and tyramine . The ginger oil emulsion 60% NPs, garlic oil emulsion 60% NPs and onion oil emulsion 60% NPs were safe and have sizes (222.6 ± 2.22 nm, 420.7 ± 36.95 nm and 202.9 ± 2.1 nm) respectively. Salmonella spp , E.coli and S.areus were the most sensitive to the three nanoparticles at 7.5%, while Klebseilla pneumonia and Enterobacter spp were the most resistant. The three nanoparticles at level 7.5% had a significant effect on the concentration of biogenic amines. As in histamine, the untreated samples were 2.3mg/kg, while in treated samples it was zero. Also tyramine was 1.19mg/kg, while in treated samples was zero , Cadaverine, was 38.59mg/kg in untreated while in treated was 2.66 , 1.5 and 1.29 mg/kg (garlic , onion and ginger ) ,Putrescine was 8.3mg/kg in untreated while in treated was 5.8 , 4.7 and 7.8 mg/kg (garlic , onion and ginger Conclusion: Using plant extract such as ginger, garlic and onion nano emulsion oils as antibacterial more effective in their action and reduction of biogenic amine formation. | ||
کلیدواژهها | ||
Keywords: Biogenic amines؛ gram negative and gram positive bacteria؛ HPLC؛ minced meat؛ natural Nano emulsions | ||
عنوان مقاله [English] | ||
تأثیر برخی از عصارههای نانو گیاه بر باکتریهای بیوژنیک مولد آمین های جدا شده از گوشت چرخ کرده | ||
نویسندگان [English] | ||
امانی عمر سلیم1؛ مروه مگدی عبدالسلام2؛ راش نقیب عبدالله حسن2؛ گهان السعید حسن3؛ زینب عبدالرحمان مهدی1 | ||
1گروه باکتریولوژی، آزمایشگاه استانی بنها، پژوهشکده بهداشت دام، مرکز تحقیقات کشاورزی، بنها، مصر | ||
2گروه بهداشت مواد غذایی ،آزمایشگاه استانی بنها، موسسه تحقیقات بهداشت حیوانات، مرکز تحقیقات کشاورزی (ARC)، قاهره، مصر. | ||
3گروه بیوشیمی، آزمایشگاه استانی بنها، موسسه تحقیقات بهداشت حیوانات، مرکز تحقیقات کشاورزی، بنها، مصر | ||
کلیدواژهها [English] | ||
آمین های بیوژن, باکتری های گرم منفی و گرم مثبت, HPLC, گوشت چرخ کرده, امولسیون های نانو طبیعی | ||
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